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Biomedical subjects

W W Wilson

Publications and source records attributed to W W Wilson.

28 records · Page 2Linked to original sources
PubMed1977

Specific muscle protein-sparing postoperative dextrose-free amino acid infusions.

The protein-sparing and ketogenic effect of postoperative infusion of maintenance solutions containing either 3.5% amino acids or 5% dextrose in 0.33 normal (N) saline was compared by nitrogen balance and urinary acetone determination in 31 patients. A specific muscle protein sparing effect was determined by quantitation of excretion of 3-methylhistidine in seven patients of the amino acid group and eight patients in the dextrose group. In addition, an extensive hormone and substrate profile was determined. It was found that amino acid infusions not only produce a less negative nitrogen balance but also significantly reduce excretion of 3-methylhistidine indicating specific preservation of the muscle compartment.

Amino Acids↗
PubMed1974

Pregelation aggregation of sickle cell hemoglobin.

Quasi-elastic light scattering was used to detect the initial stage of aggregation of hemoglobin S solutions. Aggregation of a hemolysate upon deoxygenation was found to occur at concentrations above 11 g/dl, but purified hemoglobin S solutions showed evidence of aggregation only for concentrations greater than 14 g/dl. A transition from a nonaggregated to an aggregated state was observed when the oxygen pressure in solution was reduced below a critical value. The total intensity of the scattered light during the early stages of aggregation was found to increase linearly with time. This result is consistent with a linear association process in which any size aggregate can combine with any other size aggregate.

Adult↗
PubMedDate not supplied

Creation of ultra-rare restriction sites in intact eucaryotic chromosomes mediated by bacterial methylases: an approach to sequencing and analyzing tumor and normal genomes.

The limited restriction of eucaryotic chromosomes would facilitate our understanding of the aberrant genomes of genetic diseases and cancer. We have described methods for methylating eucaryotic chromosomes embedded in agarose plugs. We now describe how Cla I methylase can be utilized in a methylation-dependent restriction cleavage with Dpn I to restrict eucaryotic genomes into a limited number of fragments. We have restricted the genomes of Saccharomyces cerevisiae at four sites and cleaved the three chromosomes of Schizosaccharomyces pombe into eleven fragments. Unlike the recently published methodologies using DNA sequences inserted into procaryotic and eucaryotic genomes, the methodologies described here use unaltered eucaryotic genomes for highly limited restriction. This methodology has applications in speeding, simplifying, and reducing the cost of sequencing the human genome.

Bacterial Proteins↗
PubMedDate not supplied

Technology assessment in healthcare: a review and description of a "best practice" technology assessment process.

BACKGROUND: Technology assessment has become a rapidly growing component of the healthcare system. It has assumed a functional role in operational settings and is rapidly impacting decisions involving purchasing, coverage, and reimbursement. This review is intended to assist the healthcare decision maker in considering the application of technology assessment in healthcare, so as to maximize the efficiency of future purchasing decisions. METHODS: This "best practice" was synthesized after identifying key institutions performing technology assessment in healthcare and analyzing their working processes, including literature review, consensus panel discussions, and expert opinion. RESULTS: We describe this best practice on a reiterative loop that consists of five processes: awareness, strategic appropriateness, analysis versus need, acquisition and implementation, and reassessment. Typical barriers to adoption of technology assessment are also identified and discussed. CONCLUSIONS: This review suggests a common terminology for the core processes involved in technology assessment, thereby facilitating a more uniform understanding among the different components of the healthcare system (i.e., payer, provider, and society) while recognizing their different perspectives.

Academies and Institutes↗