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W W Chen

Publications and source records attributed to W W Chen.

80 records · Page 5Linked to original sources
PubMed1976

Participation of a trisaccharide-lipid in glycosylation of oviduct membrane glycoproteins.

Preincubation of a hen oviduct membrane preparation with UDP-Nactyl[14C]glucosamine and bacitracin, followed by incubation with GDP-mannose, leads to formation of a chloroform/methanol (2/1)-extractable glycolipid. Treatment of the lipid with mild acid results in the release of a trisaccharide shown to have the structure beta-mannosyl-N-acetylglucosamineyl-N-acetylglucosamine. Incubation of purified trisaccharide-lipid with oviduct membranes in the presence of sodium deoxycholate, Mn2+, and GDP-mannose leads to formation of a labeled glycoprotein with an apparent molecular weight of 25,000...

Acetylglucosamine↗
PubMed1976

Pregnancy associated with renal glucosuria.

On reviewing 17,647 pregnancies over a 6-year period, 291 of 17,229 singleton pregnancies were noted to be associated with renal glucosuria. On analyzing these 291 pregnancies in 277 women, renal glucosuria was more frequently observed in those women with blood group types A and B and less in those with blood group type O. The incidence of renal glucosuria in nulliparous women, 2.8%, was significantly higher than that in parous women, 1.1%. A significantly high incidence of prematurity, 25.0%, was noted in women who persistently spilled a large amount of glucose.

ABO Blood-Group System↗
PubMed1975

A lipid-linked oligosaccharide intermediate in glycoprotein synthesis in oviduct. Structural studies on the oligosaccharide chain.

The structure of the oligosaccharide chain of the lipid-linked oligosaccharide that serves as a donor of oligosaccharide chain to proteins of hen oviduct membranes has been investigated. A [Man-14C]glycopeptide fraction was prepared from membrane glycoproteins labeled with GDP-[14C]mannose. Reductive alkaline cleavage of this glycopeptide yielded a reduced oligosaccharide that, by four criteria, was identical with reduced [Man-14C]oligosaccharide prepared from [Man-14C]oligosaccharide-lipid. The structure of the oligosaccharide chain of the [Man-14C]glycopeptide was investigated by cleavage with a specific endo-beta-N-acetylglucosaminidase, followed by treatment of the released oligosaccharide with purified al alpha-and beta-mannosidases. By this procedure it was possible to establish the structure of the cleavage product as (alpha-Man)n-beta-Man-(1 leads to 4)-GlcNAc. Similar studies were performed on the [GlcNAc-14C]oligosaccharide prepared by hydrolysis of [GlcNAc-14C]oligosaccharide-lipid. The results indicate that the structure of the intact oligosaccharide is (alpha-Man)n-beta-Man-(1 leads 4)-beta-GlcNAc-(1 leads to 4)-GlcNAc. These experiments, coupled with earlier enzymatic studies on synthesis of the glycoproteins from the lipid-linked oligosaccharide, provide strong evidence that the structure of the oligosaccharide intermediate and the oligosaccharide chain of the glycoprotein product contain the same core structure found in many secretory glycoproteins.

Animals↗
PubMedDate not supplied

Use of urine cotinine to validate smoking self-reports in U.S. Navy recruits.

Cotinine analysis of urine has been used by many researchers to determine validity of smoking self-reports. This technique is easy and inexpensive, but has not been used previously in military smoking studies. This study incorporated a random validation of self-reported smoking by U.S. Navy recruits participating in a smoking relapse program (N = 496). Results of cotinine analysis indicate only a 1% misrepresentation of actual smoking status. These results suggest smoking self-reports from U.S. Navy recruits are very good indicators of actual smoking status.

Adult↗
PubMedDate not supplied

A population-based hepatitis B vaccination coverage survey among Asian and Pacific Islander American students in Alachua County, Florida.

PURPOSE: The goal of this project was to evaluate the impact of the Florida mandatory hepatitis B vaccination law on the immunization status of Asian American and Pacific Islander (AAPI) students in Gainesville. METHODS: We (1) compared the differences of hepatitis B immunization rate between students in grades seven through nine and grades 10 through 12, (2) assessed knowledge of and attitudes towards hepatitis B vaccination of parents of AAPI students, and (3) promoted the importance of Hepatitis B vaccination among those who have not received the vaccination. PRINCIPAL FINDINGS: Two hundred and eighty-eight AAPIs were enrolled in grades seven through 12; 42 could not be contacted. Forty-seven percent of the parents (n = 116) consented to in-home interviews and gave permission to verify the immunization status. An additional 65 parents gave permission to verify the immunization status, but did not consent to be interviewed. CONCLUSIONS: As expected by state mandate, significantly more seventh, eight, and ninth graders were immunized compared to students in grades 10 through 12 (X(2) = 137.50, p < 0.0001). More than 90% of the students in the seventh, eight, and ninth grades had received all three hepatitis B vaccines. Less than 30% of the students in grades 10 through 12 received all three vaccines. Most parents (79%) said their high school-aged children had not received the vaccines because they were not required to do so. Most parents knew children needed three vaccines to be fully immunized and correctly identified the liver as the target organ. However, there was uncertainty about the mode of HBV transmission.

Adolescent↗