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Biomedical subjects

W Sterry

Publications and source records attributed to W Sterry.

At least 271 records · Page 15Linked to original sources
PubMed1989

Use of monoclonal antibodies (UCHL1, Ki-B3) against T and B cell antigens in routine paraffin-embedded skin biopsy specimens.

Diagnosis and classification of cutaneous lymphoid infiltrates are often hampered by lack of frozen tissue to allow detailed immunophenotyping. Therefore antibodies that recognize antigens resistant to routine tissue processing would be of value. In this study we present a detailed analysis of two such antibodies against CD45R antigens, UCHL1 and Ki-B3, that detect predominantly T or B cells, respectively. Tumor cells in 125 cases of various nonlymphatic skin tumors did not react with either UCHL1 or Ki-B3. Examination of 85 biopsy specimens from 13 different inflammatory skin diseases demonstrates that most reactive T cells in the skin carry the UCHL1 antigen, as controlled by simultaneous immunophenotyping in frozen tissue. With few exceptions, UCHL1 also detected the tumor cells in 28 cases of cutaneous malignant T cell lymphomas. In contrast, Ki-B3 failed to detect tumor cells in seven of eight cases of malignant B cell lymphomas of the skin.

Antibodies, Monoclonal↗
PubMed1989

Pleomorphic T-cell lymphoma and large-cell anaplastic lymphoma of the skin. A morphological, immunophenotypical, and ultrastructural study of two typical cases.

Pleomorphic T-cell lymphoma and large-cell anaplastic lymphoma are recently defined lymphoma types that both occur in the skin. This report outlines the characteristics of these lymphomas and describes the clinical, histological, immunological, and ultrastructural features as seen in two typical case histories. Cutaneous T-cell lymphomas other than mycosis fungoides and Sézary syndrome are heterogeneous; they deserve further scientific attention to obtain data about their natural history and their response to therapy.

Aged↗
PubMed1989

Large cell anaplastic lymphoma of the skin.

Large cell anaplastic lymphoma (LCAL) is a recently described entity recognized by its constant expression of the CD30 antigen and by its morphology. LCAL is classified as high grade malignant lymphoma. We report 10 patients with LCAL of the skin, three with primary LCAL and others in which it developed with cutaneous T-cell lymphoma, mainly mycosis fungoides. The immunophenotype in all but one of these cases, was that of activated T cells.

Aged↗
PubMed1989

CD4+ cutaneous T-cell lymphomas show the phenotype of helper/inducer T cells (CD45RA-, CDw29+).

CD4+ T cells are heterogenous and include at least two subsets that differ in their influence to immunoglobin synthesis, cytokine secretion pattern and immunophenotype. Among others these subsets have been designated as suppressor/inducer or naive T cells (CD45RA+, CDw29-) and helper/inducer or memory T cells (CD45RA-, CDw29+). Current theories suggest that these CD4+ T-cell subsets either reflect sequential stages of maturation before and after activation (antigen contact) or represent distinct lineages. In this study, we systematically analyzed the participation of both suppressor/inducer (CD45RA+) and helper/inducer (CDw29+) T cells in the dermal lymphohistiocytic infiltrate of various CD4+ cutaneous T-cell lymphomas. Although in peripheral blood both subsets are equally distributed, we present evidence that all CD4+ cutaneous T-cell lymphomas are of the helper/inducer T cell phenotype. These findings are of importance both for pathogenetic and clinical considerations: the presence of plasma cells in dermal infiltrates and the elevation of serum immunoglobulins in patients of mycosis fungoides may be the consequence of interleukin-4 secretion of the neoplastic CD4+ helper/inducer cells. The exclusive memory T cell phenotype of cutaneous T-cell lymphomas may be due to a general predominance of this subset in the skin, or be the consequence of cellular activation during malignant transformation.

Antigens, Differentiation, T-Lymphocyte↗
PubMed1989

Plasma cells in the dermal infiltrate of mycosis fungoides are of polyclonal origin.

There are several reports on the occurrence of immunoglobulin-producing B-cell lymphomas in Sézary's syndrome and mycosis fungoides, which may be the consequence of the helper activity of the neoplastic T-cells. Therefore we investigated skin biopsies of 50 patients with mycosis fungoides regarding the presence of plasma cells and their immunoglobulin profile. Nine of these patients had plasma cell nests, most frequently located at the lower edge of the infiltrate. IgE was detectable consistently, and IgG, IgM and IgA could also be demonstrated in the majority of these cases; kappa- and lambda-chains were present in equal amounts. Our results demonstrate polyclonal activation of plasma cells in a subgroup of mycosis fungoides patients.

Humans↗
PubMed1989

[Heterogeneity of CD4+(helper)-T-cells: role in the pathophysiology of the skin].

CD4+ cells play a central role in the immune system. Recent data show the existence of two distinct subsets of CD4+ cells in the peripheral blood of adults, which differ dramatically both in their functional capacities and in their surface phenotype. These two CD4+ subpopulations do not represent different lineages, but reflect sequential stages of maturation. As antigen contact causes transition to the subsequent subpopulation, these cells have been designated "naive" and "memory" T cells. It is remarkable that the quantitative relationship between the two subpopulations is very different within the skin from in peripheral blood: in inflammatory and in neoplastic CD4+ cutaneous infiltrates there is a predominance of memory T cells. Our own results show, furthermore, that this predominance of memory T cells is due to their selective emigration from the blood, most probably because of their high expression of several adhesion molecules.

CD4-Positive T-Lymphocytes↗
PubMed1989

Formalin-resistant leukocyte surface antigens in the diagnosis of cutaneous malignant lymphomas.

Immunophenotyping of routinely embedded lymphoma biopsies, increasingly used in nodal lymphomas, has not been systematically performed in cutaneous lymphomas. Eight monoclonal antibodies were tested directed against formalin resistant antigens (T cell biased: UCHL1, MT1; B cell biased: 4KB5, L26, Ki-B3; HLA-class II: CBL 120; activation antigens: Ber-H2; myeloid biased: Mac 387, M1) in cutaneous T (N = 29) and B (N = 10) cell lymphomas and pseudolymphomas (N = 9). UCHL1 and MT1 showed a high specificity and sensitivity. B cell-restricted antibodies L26 and 4KB5 showed the best sensitivity and specificity among the B cell-associated antibodies; Ki-B3 showed aberrant expression on T cells in some cases. Ber-H2 (CD30) was demonstrated in most cases of large cell anaplastic lymphoma and single cases of other lymphomas and lymphomatoid papulosis. Class II antigen expression, as determined by CBL 120, showed good specificity but low sensitivity, and antibodies directed against myeloid antigens did not react with tumor cells.

Antibodies↗
PubMed1989

Localized and disseminated pagetoid reticulosis. Diagnostic immunophenotypical findings.

Pagetoid reticulosis (PaRet) is a rare skin disease with an intraepidermal infiltrate of atypical lymphocytes. We performed phenotypic studies on two patients with classic localized PaRet (Woringer-Kolopp disease) and one patient with the disseminated type (Ketron-Goodman disease) and compared these with all published reports of cases; both variants show intraepidermal, highly activated, proliferating T cells with variable loss of pan-T-cell antigens, contrasting with nonactivated dermal reactive T cells. This pattern is unique among cutaneous lymphomas and can be used for diagnosis. Despite the fact that localized PaRet does not show a malignant course, recent genotypic analysis of one patient showed monoclonality. Comparable constellations are found in other diseases like lymphomatoid papulosis, which also represent monoclonal disorders of activated T cells with a benign course. To unify these seeming discrepancies, we introduce the concept of "benign cutaneous lymphomas."

Adult↗
PubMed1988

C-type particles are inducible in Se-Ax, a continuous T-cell line from a patient with Sézary's syndrome.

Se-Ax is a continuous mature T-cell line that we have established from a patient with Sézary's syndrome. An important finding was that the Se-Ax cell line required human serum for initial growth. Here we show that transfer of the permanent cell line to a medium deficient of human serum induces production of C-type retroviral-like particles with a unique morphology. Ultrastructurally, these particles are 120 nm in diameter with hexagonal shape, and show a small, centrally located round core of 30 nm. They are observed only extra-cellularly; typical budding, however, is not found. Both by serological testing and molecular analysis we substantiate the morphological conclusion that these particles do not represent common human or animal retroviruses. The inducibility of the particles may be a hint as to a possible endogenous origin.

Antigens, Differentiation↗
PubMed1988

[A non-specific vulvo-vagino-gingival syndrome].

The case of a 29 year-old patient is reported who initially complained about gingivitis and one year later developed a colpitis. After two years the patient was admitted to our hospital where inflammatory erosive changes of the gingiva, the vagina and the vulva were seen. Neither laboratory parameters nor histological results allowed an exact diagnosis of these findings. Also a Lichen ruber erosive had been excluded by the microscopic findings of a gingival and vaginal biopsy. Thus, the diagnosis of a non-specific vulvo-vagino-gingival syndrome was established, whereby antiphlogistic treatment with corticosteroids led to an improvement of the patient's symptoms.

Adult↗
PubMed1988

Quadrant distribution of dysplastic nevus syndrome.

A 59-year-old man with numerous aggregated pigmented lesions confined to the left upper quadrant of his body is described. The lesions consist of lentigines, common acquired nevi, and dysplastic nevi. In addition, within this quadrant two malignant melanomas at different stages of progression developed from dysplastic nevi. This patient presents a hitherto undescribed quadrant type of dysplastic nevus syndrome. As quadrant syndromes are due to single mutations, our observation strongly supports the concept of an autosomal-dominant trait in dysplastic nevus syndrome.

Arm↗
PubMed1987

[Torre-Muir syndrome. Sebaceous gland tumors indicate colon carcinoma and other internal malignant tumors].

The Torre-Muir syndrome belongs to a group of hereditory cancers and expresses itself in the occurrence of cutaneous gland tumors and (often multiple) carcinoma of the colon. The syndrome is probably a phenotypical manifestation of the "cancer family" syndrome, in which familial carcinoma of the colon also occurs. A case of Torre-Muir syndrome in a 43-year-old man is described. Because of the dermatological features, the colon carcinoma was diagnosed in time. Family investigations revealed another case of complete Torre-Muir syndrome, as well as a remarkable frequency of colon carcinoma in one family branch. Inheritance is autosomal dominant. The characteristic morphological features of the sebaceous gland neoplasias makes an early, often life-saving, diagnosis possible.

Adult↗
PubMed1987

[Colchicine in dermatology].

Colchicine is one of the oldest drugs known to us. During the last few years, it has been increasingly applied in the treatment of systemic dermatoses. Particularly in dermatoses mediated by neutrophils, colchicine has settled among other therapies. With regard to Behçet's disease, it has even become the treatment of choice. The present review deals with further dermatological disorders sensitive to colchicine; in addition, we mention those dermatoses which do not show any therapeutic response to colchicine. The treatment with colchicine requires profound knowledge of the pharmacokinetics and mode of action regarding this substance, as well as the possible side effects. This knowledge allows safe and responsible treatment with colchicine. It has to be considered, however, that in the Federal Republic of Germany colchicine has not been registered for dermatological indications yet.

Behcet Syndrome↗
PubMed1987

A continuous T-cell line from a patient with Sézary syndrome.

A continuous cell line, Se-Ax, from a patient with Sézary syndrome has been established. The Se-Ax cell line is IL-2 dependent, requires human serum for permanent growth, and has the following phenotype: CD1-, CD2+, CD3+, CD4-, CD5-, CD8-, CD20-, CD25+; it expresses the T9, T10, and HLA-DR antigens. This cell line reveals multiple chromosome aberrations as seen in the most abundant abnormal clone in peripheral blood. Therefore, it is not unlikely that it derives from tumor cells. A putative cytotoxic cell line derived from the same patient has only weak killer-cell activity against the autologous permanent cell line: this CD8+ cytotoxic cell line has a 14q+ chromosomal marker. The fact that the patient demonstrated no natural killer-cell or activated killer-cell activity against the Se-Ax cell line may in part explain the successful establishment of the continuous cell line from bulk culture.

Aged↗