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Biomedical subjects

W Sawicki

Publications and source records attributed to W Sawicki.

At least 37 records · Page 2Linked to original sources
PubMed1998

[Evaluation of research--reality and illusions].

Productivity and effectiveness of research are defined and their indicators discussed. Particularly, citation index (CI) and impact factor (IF) as the measures of research quality are analysed. Citation index despite its drawbacks and pitfalls is the best, among applying at present, measure of research quality. Impact factor due to its severe drawbacks is not recommended as a measure of research quality, particularly for the matching. The mixture of criteria excluding IF are propose to evaluate research quality.

Bibliometrics↗
PubMed1995

Hysteroscopy with selective endometrial sampling after unsuccessful dilatation and curettage in diagnosis of symptomatic endometrial cancer and endometrial hyperplasias.

We report the incidence of endometrial hyperplasia and endometrial cancer recognised by hysteroscopy in 202 patients with recurrent abnormal uterine bleeding. In all these women previously performed dilatation and curettage was unsuccessful. Endoscopy connected with direct biopsy allowed us to recognise in 26 (12.9%) patients pathological endometrial lesions in 19 of cases endometrial hyperplasia (with or without cell atypia) and in 7 patients endometrial cancer. The hysterosocpically recognised abnormalities in more than 70% were focal. Hysteroscopy with selective endometrial biopsy, performed after unsuccessful curettage is an essential tool in the diagnosis of recurrent abnormal uterine bleeding caused by pathological endometrial lesions.

Adult↗
PubMed1995

Transvaginal Doppler ultrasound with colour flow imaging in benign and malignant ovarian lesions.

Since early detection of ovarian cancer is difficult, most cases are diagnosed at advanced stages. The imaging diagnosis is based on pattern classification and is limited with respect to the precise determination of malignancy. High-frequency transvaginal sonography improves the ability to detect malignant ovarian tumors over that of transabdominal route, however, the predictive values are unsatisfactory because of inability to distinguish between malignant and benign tumors that have similar morphologic characteristics. The introduction of transvaginal colour flow imaging has allowed detection of low-resistance intratumoral blood vessels, characteristic of malignant tumors, and visually reflected the state of blood flow of an ovarian tumor. These two ultrasonographic methods were used for diagnosis of ovarian tumors in 65 women treated in our Department of Obstetrics and Gynecology. Waveforms of the parenchymal tumor arteries or tumor surface arteries were compared using value of the resistance index (RI). The sensitivity, specificity and accuracy of the preoperative RI in detecting malignant ovarian tumors were 100%, 94%, 95.4% respectively. The sensitivity, specificity and accuracy of preoperative suspicious sonographic findings in detecting malignant ovarian tumors were 100%, 61% and 71%. Positive and negative predictive values of colour flow imaging were 85% and 100%, whereas for grey-scale transvaginal ultrasonography they were 46% and 100% respectively. The findings of this study suggest that transvaginal colour Doppler is a method which is superior to the other methods for preoperative evaluation of ovarian malignancy.

Adult↗
PubMed1995

[Evaluation of endometrial changes in women with cyclical mastalgia].

Cyclical mastalgia which almost 40% of women suffer from is caused by inadequate ovarian hormones secretion. There are reports regarding increased number of endometrial steroid receptors in this group of women. The possibility of higher risk of endometrial cancer development in these women is very probable. 119 women with cyclical mastalgia aged 16-55 (means age 35.4 years) were investigated. Anovulatory cycles occurred in 63%. Increased estrogen action in vaginal smears was found in 24.1%, also decreased progesterone effect had been seen in 20.3% of our cases. Serum estradiol levels were elevated in 10.6% and 39.1% of the patients had lower serum progesterone levels. Mean progesterone--estrogen luteal index value was decreased to 0.99. Irregular uterine bleedings occurred in 16.8 of women (aged 41-48 years). Histological endometrium examinations revealed changes due to insufficient progesterone action.

Adolescent↗
PubMed1993

[Cardiac arrhythmias during extracorporeal shock wave lithotripsy].

Holter monitoring was performed in 55 patients (24 women, 31 men), mean age 50.7 +/- 11.3 who underwent ECG-triggered extracorporeal shock wave lithotripsy (ESWL). Patients were divided into two groups A--30 with history of cardiac disease and B--25 control. The heart rate was significantly higher before and after ESWL in the first group. The number of premature ventricular contractions was higher during ESWL in the same group. No other cardiac complication was observed.

Adult↗
PubMed1992

[Ectopic spleen in pelvis minor--a case report].

A case of ectopic spleen diagnosed as a left adnexal tumor in a 19 year old female was presented. Considering the rareness and lack of typical symptoms, diagnosis of this pathology still is very difficult, and establish most often intraoperatively. In every case of ascertainment irregular mass in minor pelvis one should differentiate it among: colonic diverticulosis, wandering kidney, coproliths, colon and mesentery tumors and ectopic spleen. We consider that ultrasonography plays the special role in these cases. In every case of stating the pathologic structure in minor pelvis it seems advisable not to content only with estimation of genital organs, but also locate such as: kidneys, liver, spleen. Such a procedure allows to state preoperative diagnosis more precisely.

Adnexa Uteri↗
PubMed1991

[Psycho-social precursors of arterial hypertension in men and women in light of clinical cohort studies].

Following two prevalence studies of arterial hypertension carried out at 5-year interval on 6487 adult inhabitants of Ochota District, Warsaw, screening for new cases was performed at the end of the five- or ten-year observation period. Interviews were collected from 472 incident and unknown prevalence cases and 499 randomly chosen non-cases regarding social and work conditions, selected behavioral traits and personal habits. The risk of hypertension was more closely associated with blue collar social status, poor economic self-assessment and repetitive type of work in women than men. In males, but not females, higher risk was connected with expressed anger followed by feeling of guilt, but no otherwise suppressed anger was predictive of hypertension. In both sexes the incidence of the disease was higher among people reporting less life events, and with less involvement in the household and/or professional activities.

Cohort Studies↗
PubMed1991

Fourier analysis of the nuclear and cytoplasmic shapes of living two-cell murine embryos.

Living two-cell mouse embryos were flushed out from the oviduct 17, 24 and 36 hours after fertilization in order to obtain cells in the G1S, early G2 and late G2 phases of the second cell cycle. The nuclei of the living cells were stained with Hoechst 33342. The coordinates of the contour shapes of the entire cells (cellular contours) were registered by contour image processing with a TV camera coupled with a computer; the contours of the nuclei were computed by means of a digitizer coupled with the computer. Fourier analysis of the cellular and nuclear contours revealed systematic modifications in the folding of the cells and nuclei in the course of the murine second cell cycle. The progression of cells through the second cell cycle was correlated with an increasing diversification of cellular and nuclear shape, with the diversification being much more pronounced in the nuclear shapes.

Animals↗
PubMed1990

In vivo staining of mouse preimplantation embryos with Hoechst 33342.

Female mice bearing secondary oocytes or preimplantation embryos of various stages were injected with Hoechst 33342 (H 33342), either intravenously or intraperitoneally and sacrificed at various intervals afterward. The dye penetrated through the wall of oviduct and stained the nuclei of its cells as well as mitotic chromosomes of secondary oocytes, the pronuclei of one-cell embryos, and nuclei of blastomeres and polar bodies. The nuclear fluorescence was observed in living cells flushed out of the oviduct as well as in those fixed in oviduct, embedded in paraffin, and sectioned. H 33342 injected in a dose of 10 micrograms/g of body weight failed to interfere with preimplantation development of stained embryos as indicated by the number of blastocysts per mouse and number of cells per blastocyst.

Animals↗
PubMed1989

Hoechst 33342 staining coupled with conventional histological technique.

Hoechst 33342 was injected either intravenously or intraperitoneally into mice which were killed 1 or 24 hr or 7, 14 or 28 days later. Various organs were fixed and paraffin embedded. Visual inspection showed that independently of the route of dye administration or survival time, distinct fluorescence of nuclei was observed in organs other than cerebral cortex. Even formic acid decalcification of bone failed to abolish the fluorescence of osteocytes. In vivo staining with Hoechst 33342 is proposed as an alternative for staining after sectioning. Cells from spleens of Hoechst 33342-injected mice or stained in vitro were injected intramuscularly into mice. Hoechst 33342-stained splenocytes could be found in deparaffinized sections at the site of injection 24 hr later.

Animals↗
PubMed1989

Heterogeneity of cytoplasmic and nuclear shape of lymphocytes during progression of chronic lymphocytic leukemia.

Peripheral blood from ten healthy subjects and from 44 patients at stages 0, I, II, III, IV of chronic lymphocytic leukemia (CLL), type B, was routinely smeared, fixed and stained by the May-Grunwald-Giemsa method. Fourier analysis of nuclear and cytoplasmic shape of smeared lymphocytes was carried out for the range 1-20 of harmonics (describing the pattern of contour folding in quantitative terms). In addition the roughness coefficients (describing the summarized measure of contour folding of an individual cell) were calculated and computer evaluated. Cytoplasmic contour shape of smeared lymphocytes in the 6-10 harmonic range discriminates well between lymphocytes of healthy subjects and those of each CLL stage. This discrimination was the result of richer folding of CLL lymphocytes. Nuclear contour shape of lymphocytes in the 6-10 harmonic range fails to discriminate between lymphocytes of healthy subjects and those of CLL, but it discriminates well between lymphocytes of various stages of CLL, with the exception of stages I/II and III/IV. When Fourier analysis was carried out on lymphocytes of combined stages I + II and III + IV, the shape differences were even more accentuated. We conclude that nuclear and cytoplasmic contour shape is a phenotypic feature of lymphocytes that is markedly modified in the course of CLL progression; this feature may be used as a new parameter in CLL.

Adult↗
PubMed1989

Number and distribution of lymphocytes in epithelium of human uteri in the menstrual cycle and menopause.

The number and distribution of intraepithelial lymphocytes (IEL) in luminal and glandular epithelium of human uterus were analysed in proliferative and secretory phase of the menstrual cycle as well as in menopause. It was found that lymphocytes were common components of luminal and glandular epithelium of human uterus. In secretory phase of menstrual cycle the IEL number of uterine luminal epithelium was on average 3.5 while in proliferative phase it decreased to 1.4 per 100 epithelial cells. In menopause the IEL number kept the level of that of proliferative phase. IEL of glandular epithelium were less numerous than those of lining epithelium and distributed evenly along the length of endometrial gland; their number failed to change in menstrual cycle.

Adult↗
PubMed1989

Bioavailability of isosorbide dinitrate from an oral therapeutic system and from tablet Sorbonit Prolongatum 20,0 in human volunteers.

The bioavailability of isosorbide dinitrate from an oral therapeutic system (OTS) and from tablet Sorbonit Prolongatum 20,0 mg in 6 volunteers employing a crossover method was studied. Concentrations of isosorbide dinitrate (1),2-mononitrate (2) and 5-mononitrate (3) are plotted as mean concentrations for all volunteers. As evident, the concentration-time curves are similar after administration of both OTS and tablet Sorbonit Prolongatum. Concentration of the nitrates in the serum is close to the maximum level starting 2 h after the drugs were administered. Such concentration is nearly constant for 7 h in the case of nonmetabolized 1 and for 9 h for its metabolites. 24 h after administration metabolites 2 and 3 were found in the serum of 5 subjects. Based on the experimental data, we calculated the areas under curves (AUC). The observed differences in AUC appeared statistically insignificant at the 95% confidence level for the two preparations studied in the case both 1 and its metabolites.

Administration, Oral↗
PubMed1988

Lymphocyte number and distribution in the rat uterine epithelium during estrous cycle and early pregnancy.

The number of intraepithelial lymphocytes (IEL) in the luminal and glandular epithelium of the uterus of virgin rats was analysed in diestrus, proestrus and estrus, and in nulliparous rats on days 5, 7 and 9 of pregnancy. IEL number was calculated either with respect to the number of epithelial cells or to the length of epithelium section. It was found that in diestrus, the number of IEL was, on average, 3.7 per 100 luminal epithelial cells or 6.7 per 1 mm of epithelium section, whereas in proestrus, it decreased to 0.9 and 1.2 IEL, respectively. On day 5 of pregnancy (before implantation) the number of IEL decreased further to 0.45 per 100 luminal epithelial cells or 0.9 per 1 mm of epithelium. On days 7 and 9 of pregnancy, IEL number further decreased in implantation sites, whereas in interimplantation sites it remained at the level calculated for day 5 of pregnancy. The population of uterine IEL consisted of small (82-99%) and large (1-18%) lymphocytes. In all stages of the estrous cycle, IEL occurred with a frequency of 68-87% in the basal region, 8-20% in the middle region and 4-12% in the apical region of the luminal epithelium width.

Animals↗
PubMed1988

Analysis of chromatin pattern in blood lymphocytes of healthy donors and in lymphoid cells of patients with chronic lymphocytic leukaemia.

The optical Fourier transformation was used to analyse the chromatin/interchromatin pattern of lymphocytes of healthy subjects and lymphoid cells of patients with chronic lymphocytic leukaemia (CLL, type B, stage O). Peripheral blood smears were prepared routinely, fixed, and stained by the Feulgen method, and the photographic images of the nuclei were quantitatively analysed. From the radial distribution of light intensity of diffractograms, several Feulgen chromatin (F-chromatin/interchromatin) descriptors were evaluated. Four showed the strongest discriminant power and these descriptors discriminated well between lymphocytes of healthy donors and lymphoid cells of CLL patients, although F-chromatin/interchromatin components of the same sizes were found in lymphocytes and lymphoid cells.

Adult↗