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Biomedical subjects

W Sauer

Publications and source records attributed to W Sauer.

32 records · Page 2Linked to original sources
PubMed1986

The ingrowth quota of autologous spongiosa from different points of removal--experimental study in sheep. A preliminary report.

The importance of the point of removal for the ingrowth quota of autologous spongiosa was examined in seven sheep. In six sheep spongiosa was taken from five different regions (proximal radius, olecranon, pelvic crest, trochanter major, and proximal tibia) and was transplanted autologously. Defined corticalis defects of the tibia were the chosen sites for the heterotopic implantations. The ingrowth quota were tested by X-ray as well as scintigraphically, histologically, and fluorescent-microscopically; one animal was taken for control. A judgement was made by quantifying scintigraphy and fluorescent-microscopic histometry. Better ingrowth quota were found for spongiosa from the pelvic crest, proximal tibia, and trochanter major as compared to qualitatively clearly worse transplants from radius base and olecranon. The results from the animal experiments prove the clinical experience.

Animals↗
PubMed1986

Establishment and growth kinetics of the mutant Ehrlich-Lettré ascites cell strain HD33 in permanent suspension culture.

Cells of a mutant in vivo subline of the Ehrlich-Lettré mouse ascites tumour (ELAT) were converted to growth in suspension culture. Kinetic analysis revealed the selective character of the conversion process; without a detectable adaptation period, a fraction of about 2 X 10(-5) of the explanted cells continued to grow in vitro. The resulting, mutant Ehrlich-Lettré ascites cell strain was designated HD33 and propagated uninterruptedly from 1974 on. The corresponding in vivo ELAT subline HD33 was derived from the HD33 ascites cell strain by intraperitoneal retransplantation. In HD33 cell suspension cultures, the population doubling time, the average intermitotic interval, as determined by videomonitoring, and the average duration of the cell cycle, as determined from percentage of labelled mitoses (PLM) data, were all measured at 15 hr. Cell loss and quiescent compartments were insignificant. The duration of the G1 phase was effectively zero. Both PLM data and [3H]/[14C] thymidine double-labelling measurements revealed an S-phase duration of between 11 and 12 hr. The G2 phase lasted 3-5 hr. The HD33 strain differs from comparable suspension strains of wild-type Ehrlich ascites cells in the insignificant role of density-dependent inhibition in growth, and the striking prolongation of the S phase which is associated with an excessive, cytoplasmic storage of glycogen by the mutant cells.

Animals↗
PubMed1985

Metabolism of 15 (p 123I iodophenyl-)pentadecanoic acid in heart muscle and noncardiac tissues.

The uptake and turnover of omega(p 123I iodophenyl-)pentadecanoic acid (I-PPA), a radioiodinated free-fatty-acid analog, was examined in the heart, lung, liver, kidneys, spleen, and skeletal muscle of rats. At 2 min post injection, a high cardiac uptake of 4.4% dose per gram had already been achieved; this was followed by a rapid, two-component, tracer clearance. The kinetics of tissue concentrations of labeled hydrophilic catabolites indicated a rapid oxidation of I-PPA and the subsequent washout of I-PPA catabolites from heart-muscle tissue. The fractional distribution of the labeled cardiac lipids compared favorably with previously reported values for 3H-oleic- or 14C-palmitic-acid-labeled myocardial lipids. Typical patterns of I-PPA metabolism were observed in tissues depending on primary fatty-acid oxidation, lipid metabolism regulation, or I-PPA-catabolite excretion. The tissue concentrations and kinetics of I-PPA and its metabolites in the heart muscle indicated that general pathways of cardiac-lipid metabolism are traced by this new gamma-emitting isotope-labeled radiopharmaceutical.

Animals↗
PubMed1984

15(p-[123I]Iodophenyl)pentadecanoic acid as tracer of lipid metabolism: comparison with [1-14C]palmitic acid in murine tissues.

Uptake and turnover of 15-(p-[123I]iodophenyl)pentadecanoic acid (I-PPA), a radioiodinated free-fatty-acid analog, was examined in heart, lung, liver, kidneys, and spleen and compared with that of [1-14C]palmitic acid (PA). High cardiac uptake of both I-PPA (4.4% dose/g) and PA (2.8% dose/g) was followed by a two-component tracer clearance. Kinetics of I-PPA were linked to those of PA in tissues with primary oxidation of free fatty acids or their preferential storage. Tissue lipids of all organs investigated were labeled concordantly by both tracers. Fractional distributions of PA and I-PPA incorporation in tissue lipids were significantly correlated. Thus general pathways of FFA tissue metabolism are traced by this radioiodinated free-fatty-acid analog. High-quality metabolic imaging of the heart is possible by means of I-PPA with conventional scintigraphic equipment or cross-sectional imaging with single photon emission computerized tomography facilities.

Animals↗
PubMed1982

[Fosfomycin concentrations in serum and bile (author's transl)].

40 patients were given a single, short intravenous infusion of 4 g fosfomycin over a period of five to ten minutes. In 23 patients, the contents of the gall bladder were removed intra-operatively 30-105 minutes after the fosfomycin infusion. In 17 patients who had undergone cholecystectomy eight to ten days earlier, bile was obtained via a T-drain 30, 60, 120, 240 and 360 minutes after the fosfomycin infusion. Fosfomycin concentrations of 1-196 mg/l were present in the bile which had been removed intra-operatively. No correlation was found between the concentration and the time of removal. Patients with the highest alkaline phosphatase in serum, however, had the lowest fosfomycin concentrations. In the patients with T-drains, the highest concentrations (93 mg/l) were found 30 minutes after the fosfomycin infusion. By the sixth hour the concentrations had fallen to 19 mg/l.

Adult↗
PubMed1977

Drug interactions during anticonvulsant therapy in childhood: diphenylhydantoin, primidone, phenobarbitone, clonazepam, nitrazepam, carbamazepin and dipropylacetate.

It is well known that the concomitant use of different drugs may alter the reactions of the body towards the individual components. This is particularly important in long-term anticonvulsant therapy which is frequently a combined therapy. By carrying out statistical analysis of more than 6000 assays of the serum levels of antiepileptic drugs an attempt was made to gain insight into the possible drug interactions. The following results were obtained: 1. There was an increase in serum levels of diphenylhydantoin when either clonazepam or dipropylacetate (short-term therapy) was given concomitantly. 2. There was a decrease in serum levels of diphenylhydantoin when carbamazepine, primidone or dipropylacetate (long-term therapy) were administered concomitantly. 3. There was an increase in the serum level of phenobarbitone when it was administered together with diphenylhydantoin. 4. There was an increase in the serum level of primidone when it was administered together with clonazepam. 5. There was a decrease in the serum level of primidone if it was administered concomitantly with either carbamazepine or dipropylacetate (long-term therapy).

Adolescent↗
PubMed1977

Morale of the urban aged: a regression analysis by race.

This research examined the degree to which previous factors shown to be related to morale were isomorphic for aged whites and aged blacks. The data consisted of a random sample of low income aged blacks and whites in Philadelphia and were collected by the late Donald P. Kent as part of the Aged Services Porject and consisted of 722 black elderly and 214 white elderly, all of whom were 65 years of age or older. The results of a regression analysis indicated that for blacks the only two significant predictors of morale were health and participation in solitary activities. For whites, in addition to health and solitary activities, interaction with family and sex were also found to be significant. It was concluded that for these data the predictors are not isomorphic between races.

Black or African American↗