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Biomedical subjects

W Sandritter

Publications and source records attributed to W Sandritter.

At least 55 records · Page 3Linked to original sources
PubMed1978

[Shock].

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Animals↗
PubMed1977

Effects of cellophane tape stripping of mouse skin on epidermal growth regulators (chalones).

Variations in epidermal chalones after a single surface application of methylcholanthrene and croton oil have been described in previous papers. This paper reports a study of the effect of adhesive tape stripping of the skin on epidermal growth regulators (G1 and G2 chalones). Pieces of adhesive tape were applied 6 times to the same area of skin in groups of mice. The short-term effects of tape stripping on epidermal DNA synthesis and on mitotic rate were studied at different intervals after stripping. Other groups of mice were killed at similar time intervals after stripping, and the treated area of skin was homogenized and extracted with water. The inhibitory effect of these extracts on normal epidermal DNA synthesis and mitotic rate was assayed in normal hairless mice. The resulting inhibitions were interpreted as an expression of the concentration of G1 or G2 chalone in the skin extracts. The first experiment confirmed that cellophane tape stripping gives rise to a short block in epidermal mitotic activity and probably also in DNA synthesis. This was followed by bimodal peaks of increased activity, the two maxima of labelling index being found on days 2 and 6, and the two maxima of mitotic rate on days 1-2 and 7. The concentrations of the two chalones in the skins of treated animals varied in inverse proportion to the alterations in the DNA synthesis and the mitotic rate, with one exception. Here there was initially a depression of the mitotic rate and a low concentration of G2 chalone. This was interpreted as a short reaction of the basel cells to the stripping trauma. It is concluded that adhesive tape stripping removes the differentiating cells and injures some basel cells, simultaneously altering the content of G1 and G2 chalones. The resulting increase in the rates of DNA synthesis and mitosis lasts only until the number of cells is high enough to produce growth-regulating substances (chalones) again. This theory may explain the changes observed. Since similar reactions are seen after both carcinogenic and co-carcinogenic chemical injury of the epidermis, the reaction pattern seems to be a general response to cell injury or cell removal.

Animals↗
PubMed1977

DNA in non Hodgkin-lymphoma--a cytophotometric study.

Cytophotometric DNA determinations on 26 cases of non-Hodgkin's lymphoma yielded the following findings: 1. Follicular centrocytic/centroblastic lymphomas (M. Brill-Symmers) and diffuse centrocytic lymphomas (lymphocytic lymphosarcoma) have a diploid DNA stem line. Diploid DNA values are observed in benign tumors, so that the assignment of these lymphomas to the group of "low grade malignancies" appears justified. 2. Lymphoblastic sarcomas show an aneuploid DNA stem line, as do 96% of all malignant tumors. 3. Lymphoid cells, plasma cells, and immunoblasts seen in immunocytomas are aneuploid. Thus these lymphomas must belong to the group of "high-grade malignant lymphomas" as regards their DNA distribution. 4. Immunoblastic sarcomas have aneuploid DNA stem lines (1 case tetraploid), in which both the lymphoid cells and the plasma cells from those immunoblastic sarcomas arising from immunocytomas show atypical DNTA distribution patterns. 5. In two cases of angioimmunoblastic lymphadenopathy, the lymphoid cells, plasma cells, and immunoblasts are aneuploid. They are thus regarded as "high grade malignancy" lymphomas. The results are discussed with respect to clinical course and prognosis. Measurements on a larger series of cases and correlation to clinical data are needed to support these results. Ultrafast DNA measurements made by flow-through cytophotometry can perhaps be helpful in the future for making the decision between a "low" or "high" grade malignant lymphoma.

Adolescent↗
PubMed1977

[Oncorna-like virus particles in the cochlear spiral ganglion of guinea pigs (author's transl)].

INTRODUCTION: Virus particles in spiral ganglion cells of the guinea pig have been grouped with herpes viruses. The purpose of this study was to evaluate this classification of the viruses and their influence on cell morphology. MATERIAL AND METHODS: 20 healthy, adult guinea pigs from different breeds were studied. The spiral ganglion was serially sectioned and examined electron micoscopically. RESULTS AND DISCUSSION: All examined spiral ganglions of several guinea pig populations from different breeds showed intracytoplasmic viruses in some granular spiral ganglion cells. According to their localization and morphology we classify these viruses with the oncorna virus group. This is not in agreement with the classification of other authors. Apparently there is a world-wide latent viral infection in guinea pigs. The accumulation of lysosomal-like vacuoles in vicinity to the viruses indicates an increased local lysosomal activity of the infected ganglion cells. Considering the otherwise normal ultrastructure of the infected cells an additional influence of these viruses on the intracellular metabolism can neither be demonstrated nor denied.

Animals↗
PubMed1977

[Ultrastructural study on formal pathogenesis of experimentally induced rhabdomyosarcomas (author's transl)].

UNLABELLED: During oncogenesis the rhabdomyosarcomas pass different stages of differentiation. In experimentally induced rhabdomyosarcomas the chronological sequence of cytoplasmatic changes can be analysed. MATERIAL AND METHODS: 10 mg 9,10-dimethyl-1,2-benz-anthracene were injected subcutaneously or intramuscularly in the hind-paw of Wistar rats with 100 gms weight. 30, 60, 105, and 150 days later the animals were killed and specimens withdrawn. The soft-tissue was prepared for light- and electron-microscopy. RESULTS: In the soft-tissue tumors, experimentally produced with DMBA, 4 different cell types can be observed, which--depending on exposition time--dominate in the histological picture: (1) The myofibrillar cells. These cells are similar to rhabdomyocytes and contain numerous cytoplasmatic fibrillae with sarcomere-like structure. (2) The myofilamentous cells. These cells contain the fibrillar structure, but without sarcomere-like arrangement. (3) The undifferentiated sarcoma cells. These cells show a pathologically changed ergastoplasm and chondrioma. (4) The matured fibrosarcoma cells are nearly similar to fibrocytes. Under the cytoplasmatic changes especially the hyperplasia of the chondrioma can be observed, in which dystrophic megamitochondriae are found. These possibly reflect the abnormal glycolysis in cancer tissue. Also mitochondrial antibodies are discussed at the form pathogenesis of this change in organelles. Regarding the change of the endoplasmatic reticulu, the annulatae lamellae can be noticed which are typical for tumor cells. Furthermore megacisterns are found, in which protein-containing material is condensed as a consequence of a disturbed synthesis and secretion.

Animals↗
PubMed1976

Preliminary clinical findings on the Kiel classification of malignant lymphomas.

In 75 patients with non-Hodgkin Lymphomas the influence of the histopathological form, as judged by the Kiel classification, on the epidemiology and prognosis of the disease was examined retrospectively. The different forms of the Kiel classification influence in a typical way the age specific incidence rates and the prognosis of the disease.

Adolescent↗
PubMed1976

[Problems in the special nosological position of keratoacanthoma. Clinical, histological and comparative Feulgen-cytophotometric studies. 3. Classification of keratoacanthomas, therapeutic consequences, conclusions].

Feulgen-cytophotometric examinations of 61 keratoacanthomas of the skin have revealed 3 types differing from each other in their DNA distribution. Keratoacanthomas type I are characterized by a diploid DNA distribution--comparable to epidermis cells. An increasing average DNA content (x) and mean distribution (Sx) compared with diploid cells are, however, conspicuous. Keratoacanthomas type II display DNA peaks in the triploid phase and increasing x and Sx. Keratoacanthomas type III show DNA tumor stem lines in the hypotetraploid, tetraploid and hypertetraploid phases, with x and Sx further increasing. The occurrence of two tumor cell populations of aneuploid localization is noteworthy in part of the keratoacanthomas. Transitional types among the tumors mentioned above are possible. Only type I does not allow cytophotometric demarcation of epidermis cells or tissue lymphocytes based on statistical analysis. The three types of keratoacanthomas can be significantly defined against each other. These cytophotometric findings explain the occurrence of clinical variations of keratoacanthomas with tumor ulceration, increased growth and recidivity. As a rule total excision and careful histological examinations are advisable. The occurrence of paraneoplastic keratoacanthomas and carcinomata disguished as keratoacanthomas is mentioned. Spontaneous healing should not be waited for despite the possible occurrence of benign keratoacanthomas.

Aged↗
PubMed1976

Effects of croton oil on epidermal growth regulators (chalones).

Variations in epidermal chalones after a single surface application of methylcholanthrene have been described in previous papers. This paper reports a study of the effect of croton oil on epidermal growth regulators (G1 and G2 chalones). Hairless mice received a single topical application of 0.2 ml 0.25% acetone solution of croton oil. Control mice received only acetone. The short-term effect of croton oil on epidermal DNA synthesis and mitotic rate was studied. Other groups of croton oil-treated and acetone-treated mice were then killed at similar time intervals, and the treated area of skin was homogenized and extracted with water. The inhibitory effect of these extracts on normal epidermal DNA synthesis and mitotic rate was assayed in normal hairless mice. The resulting inhibition was interpreted as an expression of the concentration of G1 and G2 chalones, respectively, in the skin extracts. The first experiment confirmed that a single croton oil application provokes a short block in epidermal mitotic activity and probably also in DNA synthesis. This was followed by bimodal peaks of increased activity, the two maxima of mitotic rate on days 2 and 7. The concentration of the two chalones in the skins of treated animals varied in inverse proportion to the alterations in the DNA synthesis and the mitotic rate, with one exception. There was here initially a depression both of the mitotic rate and a low concentration of G2 chalone. This was interpreted as a short, initial direct effect of croton oil on the G2 chalone present at the time of application. It is concluded that croton oil application injures and kills epidermal cells, with subsequent alterations in the content of G1 and G2 chalones. This theory may explain the changes observed. The effects of croton oil on the amount of G1 and G2 chalones in the skin are probably related to the direct, toxic, cell-killing effect of croton oil, and not to its specific cancer promoting potency.

Animals↗