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Biomedical subjects

W Sakamoto

Publications and source records attributed to W Sakamoto.

At least 109 records · Page 6Linked to original sources
PubMed1987

[A case report: primary hyperparathyroidism--comparison before and after parathyroidectomy by oral calcium tolerance test].

To evaluate the cause of hypercalciuria, we carried out the oral calcium tolerance test before and after parathyroidectomy in a patient with primary hyperparathyroidism who had recurrent and multiple nephrolithiasis. Preoperative laboratory examination showed hypercalcemia, hypophosphetamia, hypercalciuria, decrease in % tubular reabsorption of phosphorus and strikingly elevated urinary cyclic AMP excretion. The oral calcium tolerance test indicated a significantly greater increase in serum calcium (delta serum calcium: 1.4 mg/dl vs 0.8 mg/dl) and a significantly greater suppression of urinary cyclic AMP excretion (delta U-cyclic AMP:-3.56 moles/gCre vs-1.17 moles/gCre) before parathyroidectomy than after. These results showed that hypercalciuria in this case was induced not only by the significant increase in the filtrated load of calcium but by the reduction in the resorption of calcium in the distal tubule caused by the significantly suppressed parathyroid hormone effect.

Administration, Oral↗
PubMed1987

[The local blood flow of the rabbit bladder measured by electrochemically generated hydrogen gas].

The regional blood flow of the urinary bladder of rabbits was measured by electrochemically generated hydrogen gas. Then the effects of increased bladder capacity, and of systemic administration of scopolamine and neostigmine bromide on regional blood flow were examined. The local blood flow of the rabbit bladder was 75.9 +/- 8.4 ml/100 g/min (mean +/- S.E.) and it decreased by increased bladder capacity. It was also decreased by scopolamine bromide, while it was increased by neostigmine bromide.

Animals↗
PubMed1987

[A case report: surgical reconstruction of the external genitalia in adrenogenital syndrome].

This is a case report of a 5-year-old girl with congenital adrenal hyperplasia (CAH) who had clitoral hypertrophy and vagina opening at verumontanum between the bladder neck and external urethral sphincter. Female pseudohermaphroditism with CAH is the most common type of intersex problem seen in children. In females with this disorder, the internal genital organs are usually normal, but variable degrees of virilization can be observed externally. We discussed the indication of the surgical correction of ambiguous external genitalia in this syndrome in view of sexual function and cosmetic problems.

Adrenal Hyperplasia, Congenital↗
PubMed1987

[A case of nephrocalcinosis due to Sjögren syndrome associated with renal tubular acidosis].

A 39-year-old women was admitted to the hospital because of fever, lumbago and recurrent history of spontaneous stone discharge. An abdominal X-ray film demonstrated multiple calculi in the medullary positions of both kidneys and right multiple ureteral stones. Laboratory examinations showed hypergammaglobulinemia and the urine pH level was fixed around 7; nevertheless general metabolic acidosis existed. These laboratory data indicated nephrocalcinosis due to Sjögren syndrome associated with renal tubular acidosis. Right multiple ureteral stones were removed by transurethral ureteroscopy. After starting alkali therapy, neither increased nor recurrent stone formation was recognized.

Acute Kidney Injury↗
PubMed1987

[A case of giant carcinoma of the prostate].

A 74-year-old male complaining of lower abdominal mass was admitted to our hospital on August 1, 1985. He had also suffered from dysuria and bilateral lower limb edema for a year. At the time of admission, two fist-size hard masses were palpable on each suprainguinal abdomen. Remarkable venous dilatation on the surface of the whole abdomen was recognized. An enlarged stone-like hard prostate was palpable by rectal examination. Laboratory findings revealed moderately increased blood sedimentation rate and a high value of prostatic acid phosphatase. Urethrocystogram showed remarkable compression of both lateral walls of the bladder medially and the floor of the bladder was highly elevated. Computed tomography revealed almost the whole pelvic cavity occupied by the tumor which had invaded the retroperitoneal space upward as far as the level of the hilus of the kidney. Histological diagnosis was well-differentiated adenocarcinoma of prostate. Bilateral orchiectomy was performed on August 29, 1985 and he was treated with estramustine phosphate (Estracyt) 560 mg a day. Two months later, there was remarkable regression of the tumor and urethrocystogram was almost normal. Related reports are also reviewed.

Adenocarcinoma↗
PubMed1986

T-kinin is released from T-kininogen by consecutive cleavage by cathepsin E-like proteinase and 72 kDa proteinase.

Using highly purified T-kininogen and cathepsin E-like proteinase and 72 kDa proteinase in rat spleen, the release of T-kinin from T-kininogen was found to occur by consecutive cleavage by cathepsin E-like proteinase and 72 kDa proteinase. 72 kDa proteinase seems to be serine proteinase, because it was completely inhibited by diisopropyl fluorophosphate but not by pepstatin, leupeptin and bestatin.

Animals↗
PubMed1986

T-kininogen in rats with carrageenin-induced inflammation.

Specific antiserum against T-kininogen was prepared in rabbits, using T-kininogen purified from rat plasma. The antigenic activity of T-kininogen was completely lost with the release of kinin from native kininogen by cathepsin D treatment. In the determination for T-kininogen in rat plasma and pouch fluid, there was a close relationship between the single radial immunodiffusion method and the amount of total kinin released by trypsin treatment using biological assay. The kininogen levels in plasma and pouch fluid were determined and found to be related to the inflammatory responses, using a single radial immunodiffusion method. Subsequently, the increase in T-kininogen levels in plasma and pouch fluid were not in parallel with the granuloma tissue formation and exudation. However, a significant positive correlation was observed between the total T-kininogen in pouch fluid and the volume of pouch fluid.

Animals↗
PubMed1986

Glandular kallikrein, renin and tonin in tissues of diabetic and hypertensive rats.

The submaxillary gland and kidney of diabetic and hypertensive rats were compared for their content of glandular kallikrein and the activities of tonin and renin. The submaxillary glands and the kidneys of both diabetic Wistar strain and hypertensive rats contained significantly less glandular kallikrein than non-diabetic Wistar strain and hypertensive rats (reduction fron 40 to 76%). The renin activity of the kidney showed only a slight change in spite of diabetes, whereas the activity of the submaxillary gland decreased in parallel with the reduction of the kallikrein content when diabetes was induced. On the other hand, the tonin of the submaxillary gland, which has a potent hypertensive activity like renin, was not affected by induction of diabetes. However, the tonin activity in hypertensive rats was significantly higher (p less than 0.001) than that in the normotensive rats (His-Leu, 168.7 +/- 10.1 vs. 131.5 +/- 17.3 nmol/min X mg protein).

Animals↗
PubMed1985

Glandular kallikrein content in tissues of diabetic and hypertensive rats measured by enzyme immunoassay.

The contents of glandular kallikrein in the submaxillary gland and pancreas of normal, diabetic and hypertensive rats were compared using a specific enzyme immunoassay. The kallikrein levels in the submaxillary gland and pancreas of the diabetic rats were significantly lower than those of normal rats. On the other hand, the submaxillary and pancreatic levels in hypertensive rats tended to be higher than in normal rats.

Animals↗
PubMed1985

[Clinical effectiveness of pipemidic acid on gonorrhoeal urethritis].

Twenty cases of gonorrhoeal urethritis were orally treated with pipemidic acid (PPA). The results are as follows. The clinical effect of PPA was excellent in 9 cases and good in 6 cases, the efficacy rate being 75%. The usefulness of PPA was satisfactory in 12 cases (60%). No side effects were observed. The minimal inhibitory concentrations against 14 isolated Neisseria gonorrhoeal strains ranged from 0.78 to 3.13 micrograms/ml with a median value of 1.56 micrograms/ml. A beta-lactamase-producing strain was observed among the 14 strains and the case infected by this strain was successfully treated with PPA. These results indicate that PPA is a useful drug for the treatment of gonorrhoeal urethritis.

Adult↗
PubMed1985

[Blind-ending bifid ureter: a case report].

This is a report on a case of blind-ending ureter. The patient was a 56-year-old female who had complained of a pain in the left flank. There was no urinary tract infection. Excretory urography revealed a left blind-ending bifid ureter. It was resected and the removed specimen was about 8 cm in length. Histologically, it had all layers of the ureteral structure, but no renal tissue was found. The post-operative course was uneventful. We collected 55 cases of blind-ending bifid ureters reported in Japan including our own and discussed the difference between blind-ending bifid ureter and ureteral diverticulum.

Female↗
PubMed1985

[Myelolipoma of the adrenal gland: a case report].

A case of myelolipoma of the adrenal gland is reported. The patient was a 50-year-old male who was obese and robust, and complained of right flank pain. Laboratory investigation of adrenal functions were within normal levels. Excretory urography showed a large radiolucent mass in the right upper quadrant, displacing the right kidney to a lower position. Angiography demonstrated a large hypovascular mass. CT scan showed a large mass with low density in the right retroperitoneal space. The tumor was surgically removed through a lumbar incision, and was 800 gm in weight and well capsulated. A pathological study of the tumor disclosed adrenal myelolipoma consisting of mature fat cells and myeloid elements. Myelolipoma of the adrenal gland is rare and 33 cases of adrenal myelolipoma from the English and Japanese literature including this case were reviewed and analyzed statistically.

Adrenal Gland Neoplasms↗
PubMed1984

The kinetics of blood coagulability: fibrinolytic and kallikrein-kinin system at the onset and during labor.

The relationship between the kallikrein-kinin system, the coagulation system, and the fibrinolytic system was evaluated on 58 patients during pregnancy, labor and puerperium. Also included in this study were 7 patients with premature placental separation. The most prominent changes were those in the kallikrein-kinin system. After the onset of labor, prekallikrein decreases rapidly which may trigger changes in the blood coagulation and fibrinolytic system. The three systems have a close interrelationship possibly affecting uterine contractility during labor and delivery.

Adult↗
PubMed1984

Purification and characterization of rat low molecular weight kininogen.

Low molecular weight (LMW) kininogen was isolated from pooled rat plasma by chromatography on DEAE-Sephadex A-50, CM-Sephadex C-50, Blue-Sepharose CL-6B, and Sephadex G-100. It was shown to be homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoelectrophoresis. The molecular weight of rat LMW kininogen was determined to be 72,000 by SDS-PAGE. The LMW kininogen contained 83.5% protein, 4.0% hexose, 5.5% hexosamine, and 2.7% sialic acid. Kinin liberated from LMW kininogen by trypsin treatment was identified as an Ile-Ser-bradykinin(T-kinin) by analysis involving ion exchange column chromatography on CM-Sephadex C-25 and high performance liquid chromatography on a reverse-phase column (ODS-120T). LMW kininogen formed kinin with rat submaxillary gland kallikrein, but the kinin liberated was only 14% of the total kinin content, that is, that released by trypsin. In order to determine the immunochemical properties of LMW kininogen, specific antiserum was prepared in rabbits. The antiserum cross-reacted with high molecular weight (HMW) kininogen, but spur formation was observed between the LMW and HMW kininogens. The kininogen level in rat plasma was estimated to be 433 microgram/ml by a quantitative single radial immunodiffusion test.

Animals↗