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W S Wilson

Publications and source records attributed to W S Wilson.

52 records · Page 3Linked to original sources
PubMed1971

Quantitation of serum lipoproteins by electrophoresis on agarose gel: standardization in lipoprotein concentration units (mg-100 ml) by comparison with analytical ultracentrifugation.

Lipoprotein electrophoresis on agarose gel has been modified to allow estimation of the absolute quantity of each fraction. The reproducibility of the method is illustrated by 12 determinations in a single day on serum from one normal subject: mean total dye uptake was 302 +/- 9 (sd "corrected dye units," and the percentages of beta-, pre-beta, and alpha-lipoprotein were 56.1 +/- 0.9, 29.1 +/- 0.4, and 14.8 +/- 0.7, respectively. Reproducibility over a period of 8 months was also demonstrated. Serum lipoproteins of five normal and 15 hyperlipidemic individuals determined by this technique were compared with values obtained by analytical ultracentrifugation. The correlation coefficients were: 0.993 for pre-beta-LP vs. VLDL, 0.978 for beta-LP vs. LDL, and 0.867 for alpha-LP vs. HDL. Lipoprotein values obtained by paper electrophoresis were also correlated with those of the analytical ultracentrifuge, but to a lesser degree (r = 0.956, 0.691, and 0.786, respectively). Values for LDL and VLDL which were measured by refractometry after preparative ultracentrifugation were very similar to those obtained from the analytical ultracentrifuge. Serum triglyceride concentration was highly correlated (r = 0.972) with the agarose values for pre-beta-LP; serum cholesterol concentration was correlated (r = 0.673) with beta-LP. It is proposed that the standard curves of the comparisons with the analytical ultracentrifugal values be used to convert the corrected dye units of electrophoresis on agarose gel to mg/100 ml of specific lipoprotein.

Adult↗
PubMed1969

The effects of phenobarbitone, leptazol, dexamphetamine, iproniazid, imipramine, LSD, chlorpromazine, reserpine and hydroxyzine on the in vivo levels of adenine nucleotides and phosphocreatine in the rat brain.

1. Enzymic and ion-exchange chromatographic analyses were used to measure adenosine triphosphate (ATP), diphosphate (ADP) and monophosphate (AMP) in brain extracts from rats treated with a wide range of centrally acting drugs. Phosphocreatine (PC) was assayed by the acid molybdate method.2. An anaesthetic dose of phenobarbitone caused an increase in brain levels of ATP and PC, and a reduction in ADP and AMP. A convulsant dose of leptazol gave rise to precisely opposite effects. Subanaesthetic (hypnotic) and subconvulsive doses of the two drugs, respectively, produced no alterations in brain nucleotide levels.3. Among the psychotropic drugs, dexamphetamine, LSD and hydroxyzine, at the doses used, caused no changes in brain levels of the adenine nucleotides. Iproniazid and imipramine caused slight increases in the ATP level and ATP / ADP ratio, respectively. Chlorpromazine failed to give rise to any effect in the nucleotides 3 hr after administration, but produced a rise in brain ATP after 6 hr. Reserpine, on the other hand, caused a fall in the ATP/ADP ratio 6 hr after injection.4. These results indicate that some psychotropic drugs can cause small changes in the rat brain ATP/ADP ratio but do not support claims by certain workers that such changes correlate closely with the behavioural effects of these drugs.

Adenine Nucleotides↗