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Biomedical subjects

W Rudnicka

Publications and source records attributed to W Rudnicka.

At least 73 records · Page 4Linked to original sources
PubMed1983

Antimonocyte serum. Preparation and characterization.

Cell suspensions enriched with monocytes were obtained from the blood of Vietnam normal pig and rabbits injected with avirulent Welshimer strain of L. monocytogenes by velocity sedimentation on BSA gradient and separation on Ficoll-Triosil gradient. They were used for immunization of rabbits and a sheep, respectively. The antisera specific for monocytes (RAMS and SAMS) were obtained by scrupulous absorption with lymphocytes and granulocytes.

Animals↗
PubMed1983

Antimonocyte serum. The effect on blast transformation, RFC and PFC formation.

The paper is a continuation of the previous experiments. The results show that the rabbit and sheep antisera specific for monocytes after absorption with leukocytes deprived of phagocytosing cells do not affect E-rosetting cells and do not impair the blast transformation of normal leukocytes stimulated by PHA in vitro. However, they reduce the number of EAC rosettes formed by leukocytes and they decrease the ability of leukocytes to produce antibodies for SRBC.

Animals↗
PubMed1981

Anti-Pseudomonas immunoglobulin I. Immunization of sheep.

The production of sheep Pseudomonas immune serum is described. The animals were immunized with Pseudomonas vaccine prepared from strains of Pseudomonas aeruginosa belonging to seven immunotypes of Fisher's schema. Immune sera obtained from hyperimmunized sheep are characterized by a high level of stabel antibodies with a good protective activity for mice against infection with Pseudomonas aeruginosa. It has been demonstrated that sheep may be used for a long time as a source of immune serum.

Animals↗
PubMed1980

Suppressor activity of blood lymphocytes from guinea-pigs treated with ALS.

PHA-induced transformation of guinea-pig lymphocytes was profoundly inhibited by the injection of antilymphocyte serum (ALS). This effect, seen 24 h after ALS administration, disappeared within 3 days. Lymphocytes of animals injected with ALS 24 h earlier inhibited the blast transformation of autologous normal lymphocytes. The supernatants of short term cultures of such cells, even after pre-treatment with puromycin, exhibited the same inhibitory activity. It is suggested that the immunosuppressive effect of ALS is not limited to its direct action on lymphocytes.

Animals↗
PubMed1978

Properties of antisera against lymphocytes of nude mice.

The activity of rabbit antisera against nu/nu BALB/c lymphocytes was estimated in vivo and in vitro. It was found that anti-lymphocyte serum (ALS) against nu/nu lymph node cells suppressed the alloantigen reaction and the spontaneous rosette-forming cell (sRFC) or plaque-forming cell (PFC) formation for T-dependent (sheep red blood cells) and T-independent (lipopolysaccharide) antigens. ALS against nu/nu spleen cells affected only the sRFC and PFC for T-independent antigen. The former serum exhibited a high cytotoxicity for the suspensions enriched or depleted in B cells, while the latter was more cytotoxic for the suspension enriched in B cells. This may indicate that ALS anti-nu/nu spleen cells is specific for B lymphocytes, and ALS anti nu/nu lymph node cells is directed not only to B cells but also to a subpopulation of T lymphocytes. It may suggest the existence of a subpopulation of T lymphocytes in nu/nu lymph node cells.

Animals↗
PubMed1978

Properties of antisera against lymphocytes of nude mice.

This paper reports results of a study on the activity of rabbit antisera against nu/nu Balb/c lymphocytes in vivo and in vitro. It was found that ALS against nu/nu lymph node cells suppressed the alloantigen reaction and the sRFC or PFC formation for T-dependent (SRBC) and T-independent (LPS) antigens. ALS against nu/nu spleen cells affected only the sRFC and PFC for T-independent antigen. The former serum exhibited a high cytotoxicity for the suspensions enriched or depleted in B cells while the latter one was more cytotoxic for the suspension enriched in B cells. It indicates that ALS anti nu/nu spleen cells is specific for B lymphocytes and ALS anti nu/nu lymph node cells is directed not only to B cells but also to a subpopulation of T lymphocytes. It suggests the existence of a subpopulation of T lymphocytes in nu/nu lymph node cells.

Animals↗
PubMed1977

The estimation of lymphotoxic activity of ALS by spectrophotometric method.

Saijo's method of quantitation of the cytotoxicity of antiserum for tumor cells is adapted to estimation of lymphotoxic activity of antilymphocyte sera. The method is based on spectrophotometric measurement of an amount of trypan blue dye entering the lymphocytes damaged by action of ALS. Several possibilities of determination of the lymphotoxic titers of ALS are suggested.

Animals↗