Ofloxacin, one of the new group of quinolone antibiotics, was investigated with respect to its possible interactions with different parameters of the immune system. The study was performed in vivo on Balb/c-mice treated for seven days with different doses of the antibiotic and in vitro on human phagocytes from peripheral blood. It was found that ofloxacin does not affect cellular or humoral immune responses in mice. Moreover, human phagocytic cells exposed in vitro even to high concentrations of the drug did not show any significant changes in their function. On the other hand, it was observed that subinhibitory concentrations of ofloxacin induced an increased susceptibility of Staphylococcus aureus 511-Jena to the bactericidal activity of phagocytes.

Animals↗

PubMed1986

Effects of ciprofloxacin on the humoral and cellular immune responses in Balb/c-mice.

The effects of a 7 days chemotherapy with ciprofloxacin on the humoral and cellular immune responses in Balb/c-mice were examined. Ciprofloxacin-doses used were calculated on a body weight basis from therapeutic dosages in humans. In a dose-dependent way IgM as well as IgG responses were significantly increased. The delayed-type hypersensitivity reaction to oxazolone was not significantly changed. In in vivo as well in vitro experiments ciprofloxacin showed no modulatory activity on the concanavalin A and LPS-induced proliferative activities of mouse spleen cells.

Animals↗

PubMed1986

Effect of streptolysin S from Streptococcus pyogenes on contact sensitivity in mice.

Streptolysin S, a hemolytic toxin produced by strains of Streptococcus pyogenes, was examined for its effect on cellular immune reaction in mice. The toxin given intraperitoneally for six consecutive days did not influence intensiveness of delayed hypersensitivity to oxazolone which has been used as a model of cellular immune reaction. Streptolysin S injected subcutaneously, closely to lymph nodes directly involved in immune response, markedly suppressed delayed hypersensitivity. Significant inhibition of lymphocyte proliferation by streptolysin S was observed both in vivo as well as in vitro experiments.

Animals↗

PubMed1986

Opsonin independent interaction of Klebsiella strains with human polymorphonuclear leukocytes.

Nine encapsulated Klebsiella strains with different types of fimbriation and their nonencapsulated mutants were tested for their stimulatory potency for human polymorphonuclear leukocytes in the absence of opsonins. The luminol chemiluminescence assay was used for these experiments. It could be shown that the interaction between Klebsiella bacteria and human leukocytes is rather complex depending not only on the presence of capsules but also on the hydrophobicity of Klebsiella surface and on the type of fimbriation existing.

Fimbriae, Bacterial↗

PubMed1986

Investigations on opsonin-independent antistaphylococcal activity of human neutrophilic granulocytes, monocytes and lymphocytes.

Using cells from 20 healthy human donors the opsonin-independent staphylococcal killing abilities of peripheral blood neutrophilic granulocytes, monocytes and lymphocytes were studied. S. aureus strains T 1 and T 14, S. sciuri strain S I, S. saprophyticus strain S III and S. simulans strain S V were selected for these experiments because of their differing cell wall composition, surface hydrophobicity, protein A content and extracellular enzyme activity spectrum. We could demonstrate a very individual activity range of these cells against staphylococci. On the other hand each staphylococcal strain examined behaved in a very typical way in its susceptibility against the killing by human phagocytes and lymphocytes. Repeated tests in monthly intervals showed that the staphylococcal killing activity range of phagocytes and lymphocytes of a certain healthy donor remains constant and stable.

Humans↗

PubMed1986

[AIDS--acquired immunodeficiency syndrome].

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Acquired Immunodeficiency Syndrome↗

PubMed1986

[Use of computers in the field of human nutrition].

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Computers↗

PubMed1985

Small-cell lung cancer and immunochemotherapy with Propionibacterium granulosum KP 45.

Seventy-nine patients with small-cell lung cancer were treated with vincristin, methotrexate, and cyclophosphamide in inductive therapy and with methotrexate, cyclophosphamide, and procarbazine in maintenance therapy. Patients were divided at random into two groups: one group received chemotherapy alone and the second group was additionally subjected to systemic immunotherapy with Propionibacterium granulosum strain KP-45. In general, differences in the frequency of therapy response and in duration of remission could not be stated between the two groups of patients, but patients responding to chemotherapy showed a significantly longer remission time and lower complication rates. This benificial effect of chemoimmunotherapy is not related to a direct antitumor activity of the immunomodifier used, but to the lowered risk of myelosuppression and infections. Immunomodulation in combination with chemo- and/or radiotherapy can be recommended for the treatment of small-cell lung cancer.

Adult↗