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Biomedical subjects

W Rohde

Publications and source records attributed to W Rohde.

At least 181 records · Page 10Linked to original sources
PubMed1978

Polyethylene glycol-mediated infection with avian sarcoma viruses.

In this communication we report that fusion of viral and cellular membranes by polyethylene glycol is a convenient approach to overcoming genetically determined resistance to infection. Using this method, avian sarcoma virus-transformed mammalian cells have been produced which serve as useful model systems for the study of Rous sarcoma virus-specific tumor antigens.

Animals↗
PubMed1978

The structure of the Rous sarcoma virus glycoprotein complex.

The viral envelope glycoprotein gp85 is released from purified Rous sarcoma virus by treatment with 2-mercaptoethanol, while the second surface antigen gp35 remains associated with the membrane of intact virus particles. The data represented substantiate and extend previous observations on the Rous sarcoma virus glycoprotein complex.

Avian Sarcoma Viruses↗
PubMed1978

Minor nucleic acids in influenza virus.

A nucleic acid fraction consisting of RNA and DNA sequences with an apparent mol. wt. of 1.4 to 1.5 x 10(6) is present in minor amounts in purified influenza virus. The RNA is virus-specific and in the case of fowl plague virus (FPV) contains sequences of genes 2 and 7 which code for one of the proteins constituting the polymerase complex and for the matrix protein respectively.

DNA↗
PubMed1978

Bovine and ovine leukemia viruses. I. Characterization of viral antigens.

A comparative study on several virus-specific antigens of bovine and ovine leukemia viruses is presented. In addition to the major immunologically reactive, nonglycosylated antigen p21, which has a molecular weight of 21,000, both viruses share two glycoproteins of apparent molecular weights of 60,000 (gp60) and 32,000 (gp32), respectively. Immunological cross-reaction suggests that ovine and bovine leukemia viruses are genetically highly related.

Animals↗
PubMed1978

Studies on the time and localization of the puberal desensitization to oestrogen in female rats.

Immature and postpuberal female rats were ovariectomized at 20 or 27 days of age or on the day of the first vaginal oestrus and chronically implanted with oestradiol benzoate (OB) and cholesterol at the ratios of 1 : 60, 1 : 120 or 1 : 240 on the day following castration. Autopsy was performed on day 6 after implantation and the plasma LH concentration determined by radioimmunoassay. Whereas 1 : 60 and 1 : 120 implants of OB and cholesterol placed into the hypothalamic ventromedial-arcuate region depressed the castration-induced elevation of the LH level before and after puberty, the 1 : 240 mixture was effective only in immature rats, but not after vaginal opening and the first ovulation had occurred. A similar trend was recorded after implantation of OB into the cortical amygdaloid nucleus (CAN). However, the oestrogen dose had to be doubled to get comparable results. Bilateral lesioning of the CAN or deefferentation of the mediocortical amygdala by transection of the stria terminalis did not distinctively influence the LH-suppressing effect of daily s.c. injections of 0.1 or 0.05 microgram OB/100 g b. w. in prepuberal rats. The findings demonstrate a sudden change in the hypothalamic threshold to the gonadotrophin-inhibiting effect of oestrogen over a narrow range of time near the onset of puberty. They furthermore suggest that the mediocortical amygdala is not involved in possible extra-hypothalamic control of the puberal desensitization process.

Animals↗
PubMed1978

[A radioimmunoassay for plasma 11-desoxycortisol and its use in the rapid metopirone test].

A radioimmunoassay for the measurement of 11-deoxycortisol in plasma is described. Antiserum against 11-deoxycortisol was produced by immunizing rabbits with the 21-hemisuccinate of 11-deoxycortisol coupled to bovine serum albumin. The method does not require chromatography but instead makes use of a simple extraction procedure which, in combination with the antibody characteristics, is relatively specific for the 11-deoxycortisol determination. The smallest amount measurable is 5 pg. The intra-assay coefficient of variation was 6.3% before metopirone and 7.2% after metopirone. The inter-assay coefficient of variation was 12.5% before metopirone and 10.3% after metopirone. Pituitary-adrenal reserve was evaluated in control and hypopituitary subjects by a simple midnight metopirone test.

17-Hydroxycorticosteroids↗