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Biomedical subjects

W R Timperley

Publications and source records attributed to W R Timperley.

97 records · Page 6Linked to original sources
PubMedDate not supplied

Carcinoid tumour arising in a recurrent intradural spinal teratoma.

We report the case of a recurrent intradural spinal teratoma in a 44-year-old woman. Histological examination showed the lesion to be composed entirely of mature elements including an argyrophil carcinoid tumour. Electron microscopy showed the neoplastic cells to possess the ultrastructural features of a foregut type APUDoma. Gamma enolase, a marker of APUD cells, was demonstrated in the neoplasm by the peroxidase-antiperoxidase technique. This method was also used to demonstrate the presence of cytokeratins in the neoplastic cells and the squamous and columnar epithelia within the teratoma. These results are consistent with the suggestion that APUD cells of foregut type may be of endodermal rather than neuroectodermal origin.

Adult↗
PubMedDate not supplied

Fibrosarcoma arising in a lumbosacral meningocoele.

A 53 year old woman presented with enlargement of a sacral meningocoele and pain in the back and legs. She died from a pulmonary embolus. Fibrosarcoma, apparently arising in the sacral region, involved the leptomeninges and nerve roots as far rostrally as the pons. Nine previously reported cases are discussed.

Female↗
PubMedDate not supplied

Steroid hormone receptors in pituitary adenomas: a biochemical, immunohistochemical and morphometric study on cryostat sections.

Oestrogen receptors and progesterone receptors were measured by an isoelectric focussing technique in cytosols from cryostat sections of eight human pituitary adenomas. Cryostat sections adjacent to the assayed sample were stained for anterior pituitary hormones using the peroxidase-antiperoxidase technique, and the cellularity of each neoplasm was calculated with the aid of a computerized image analysis system. The results of ER and PR assays were adjusted to compensate for variations in cellularity. ER were present in three prolactin adenomas, one growth hormone adenoma and one gonadotrophin adenoma. The latter also contained PR. Steroid hormone receptors were not detected in two null cell adenomas and one non-secretory oncocytoma. The results support the suggestion that antihormonal chemotherapy may be valuable in the treatment of certain pituitary adenomas.

Adenoma↗
PubMedDate not supplied

Monoclonal antibody to aldolase C: a selective marker for Purkinje cells in the human cerebellum.

A monoclonal antibody to the glycolytic enzyme aldolase C was used in an immunoperoxidase technique on unfixed and formalin-fixed paraffin-embedded tissues from the human central nervous system (CNS) and other tissues. No staining outside the CNS was detected. In the central nervous system the antibody gave a selective and intense staining of Purkinje cell perikarya, axons and dendrites in both fixed and unfixed tissues. In the human fetal brain, positive staining of Purkinje cells was seen from 35-36 weeks' gestation onwards. This coincides with the conversion of Purkinje cells to a tetraploid state, and may reflect increased glycolytic activity accompanying the rapid dendritic growth at this stage of development. Preliminary investigations on a variety of disease states suggest that this antibody may be useful in studies of ischaemic damage, congenital abnormalities and degenerative conditions affecting the cerebellum.

Adult↗
PubMedDate not supplied

Growth hormone-secreting pituitary adenoma of mixed cell type: a histological, ultrastructural and immunocytochemical study.

A case of a pituitary adenoma in an acromegalic man is presented in which growth hormone secretion was demonstrated by explant culture. Light microscopy showed the tumor to consist of acidophil "signet ring" cells which contained growth hormone on immunocytochemical studies. Electron microscopy showed a mixed population of densely and sparsely granulated growth hormone adenoma cells. This latter feature has never previously been described, and may represent dual cellular differentiation from an acidophil stem cell precursor.

Acromegaly↗
PubMedDate not supplied

Enolase isoenzymes in benign and malignant melanocytic lesions.

The distributions of the alpha, beta and gamma subunits of enolase were studied in 34 melanocytic lesions using the peroxidase-antiperoxidase (PAP) technique. Melanocytes in benign neoplasms stained moderately strongly for both alpha enolase and gamma enolase but staining for beta enolase was consistently negative. Staining for alpha enolase was always stronger than that for gamma enolase. There are some indications that with increasing degrees of dedifferentiation there was a reduction of gamma enolase activity, least differentiated cells being completely negative. Ocular melanomata showed similar changes to those seen in the skin. The enolase isoenzyme composition of tumour extracts was assessed biochemically on a limited number of cases and these results are discussed in relationship to the immunohistochemical studies.

Humans↗