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Biomedical subjects

W R Slade

Publications and source records attributed to W R Slade.

33 records · Page 2Linked to original sources
PubMed1979

Sarcoid of the nervous system.

Sarcoid is a granulomatous disease of undetermined etiology characterized by the presence of epithelioid cell aggregates without caseation which proceeds to conversion to hyaline fibrous tissue or resolution. The sites of nervous system involvement include the meninges, cranial, and peripheral nerves, hypothalamus and pituitary gland, muscles, and, more rarely, brain and spinal cord parenchyma. In nervous system involvement there is usually cerebrospinal fluid lymphocytic pleocytosis and elevated protein but these findings are not specific. When other systems are involved with sarcoid the diagnosis of neurosarcoidosis is obvious. When an unusual neurological symptom complex presents, sarcoid should be considered. The author reviews the literature and presents 22 cases of sarcoid involvement of the nervous system.

Adrenal Cortex Hormones↗
PubMed1977

An extended genetic recombination map for foot-and-mouth diseases virus.

The original foot-and-mouth diseases virus recombination map (Lake, Priston & Slade, 1975), which include 35 mutagen-induced ts mutants, has been extended both in detail and size by the mapping of a further 33 ts mutants (9 mutagen-induced and 24 spontaneous). The size increase from 0.57% to 3.27% maximum recombination fequency was principally due to the use of a new standardization technique for recombination fequencies but, in addition, the original map distance was increased by approx. 30% due to the mapping of new mutations. As in the original map, there was a marked concentration of mutations near the guanidine (gs) locus, i.e. 83% of the mutants had mutations in the third of the map adjacent to the gs locus.

Aphthovirus↗
PubMed1975

Evidence for recombination between two different immunological types of foot-and-mouth disease virus.

Recombination was observed between temperature-sensitive (ts) mutants of two immunological types of foot-and-mouth disease virus which were distinguishable by two marker characteristics in addition to their antigenic type. Putative ts+ recombinants were isolated and the segregation patterns of their marker characteristics examined. The results are discussed in terms of the origin of new sub-type strains.

Antigens, Viral↗
PubMed1975

Temperature-sensitive mutants of foot-and-mouth disease virus: the isolation of mutants and observations on their properties and genetic recombination.

A number of temperature-sensitive mutants were isolated from two strains of foot-and-mouth disease virus (FMDV). Various properties of the mutants were examined including comparative growth curves at permissive and restrictive temperatures, cut-off temperatures, thermal lability and pH sensitivity. Recombination was observed between various pairs of mutants of FMDV strain Pacheco. It occurred early in the growth cycle and the proportion of recombinants remained constant thereafter. Maximum recombination was achieved if the input multiplicity of each virus was 6 p.f.u./cell or greater, provided the ratio of the input multiplicities did not vary by more than a factor of two. Day-to-day variations could be substantially reduced by normalizing recombination frequencies in terms of a standard cross. The results suggested that genetic mapping was possible with two-or three-factor crosses.

Animals↗
PubMed1975

A genetic recombination map of foot-and-mouth disease virus.

Sixty ts mutants were isolated from the Pacheco strain of type O foot-and-mouth disease virus after treatment with either 5-fluorouracil or hydroxylamine. The conditions affecting recombination and assay of the ts+ recombinants were standardized. Using two ts mutants resistant to guanidine, three-factor crosses, supported by two-factor crosses, located 34 of the mutations in a linear arrangement. The recombination frequencies between certain pairs of mutations were additive. The guanidine character of the two resistant mutants mapped as a single site mutation and was located near the middle of the map.

Animals↗