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Biomedical subjects

W R Fish

Publications and source records attributed to W R Fish.

24 records · Page 2Linked to original sources
PubMed1982

Purine metabolism in the bloodstream forms of Trypanosoma gambiense and Trypanosoma rhodesiense.

Bloodstream forms of Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense are incapable of de novo purine synthesis. Purine bases are converted directly to ribonucleotides and with the exception of guanine, are stable. Guanine is incorporated directly into ribonucleotides and also deaminated to xanthine. Purine ribonucleosides are hydrolyzed rapidly; these reactions may limit their incorporation since purine bases label the nucleotide pools more efficiently than do ribonucleosides. The apparent order of salvage efficiency for ribonucleosides is adenosine greater than inosine greater than guanosine greater than xanthosine for both organisms. T. b. gambiense salvages purine bases in the same order, while T. b. rhodesiense salvages purine bases in the order hypoxanthine greater than adenine greater than guanine greater than xanthine.

Animals↗
PubMed1982

Some Phytomonas and Herpetomonas species form unique iso-branched polyunsaturated fatty acids.

Four trypanosomatid flagellates of the genera Phytomonas and Herpetomonas have been found to carry out the de novo biosynthesis of a variety of iso-branched, C18, C20 and C22, polyunsaturated fatty acids, with 2-5 methylene-interrupted double bonds, which have not been described heretofore from natural materials; iso-C18 delta 6,9, iso-C18 delta 9,12, iso-C20 delta 8,11,14, iso-C 20 delta 5,8,11,14, iso-C22 delta 4,7,10,13,16. Identifications were based upon combinations of chromatographic, chemical degradative, mass spectrometric and proton nuclear magnetic resonance spectrometric techniques. Under appropriate culture conditions, 85% of the total fatty acids of the organisms were branched. The subject trypanosomatids are recommended as model organisms with which to investigate influences of the physical properties of phospholipid fatty acyl groups on eukaryotic cell membrane functions.

Animals↗
PubMed1981

The cyclopropane fatty acid of trypanosomatids.

Cis-9,10-Methyleneoctadecanoic acid, one of a group of cyclopropane fatty acids commonly found in bacteria but not in eukaryotic cells, has been identified in the phosphatidylethanolamines of 27 isolates representing 5 genera of trypanosomatid flagellates (Crithidia, Leptomonas, Herpetomonas, Phytomonas, Leishmania). Its presence did not appear to be associated with endosymbiotic or other microbiol associates. It was absent from 12 isolates of the genera Blastocrithidia, Endotrypanum and Trypanosoma.

Animals↗