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Biomedical subjects

W Pohle

Publications and source records attributed to W Pohle.

At least 73 records · Page 4Linked to original sources
PubMed1979

Fucose incorporation into rat hippocampus structures after acquisition of a brightness discrimination. A histoautoradiographic analysis.

Using a brigthness discrimination model in rats, the labeling of discrete hippocampus formation structures was studied after intraventricular application of [3H]-fucose. This substance was injected 5 min before training as well as 5 min, 3, 7 and 23 h after training, the pulse period lasting 120 min in all cases. A significantly training-related enhanced labeling of CA1, CA3 and CA4 cell bodies and fibres revealed that a biphasic time course occurring when radioactive fucose was applied 5 min before training and 7 h after training, whereas the increased labeling of area dentata structures was evidenced only after application of radioactive fucose 5 min before and after training. In all structures under investigation the training-related increase in labeling was more pronounced in the fibre layers than in the pyramidal and granular cell bodies.

Animals↗
PubMed1978

[Laparoscopic findings in aged patients (author's transl)].

A group of 165 geriatric patients is compared with a control group of 114 younger patients concerning different frequency of laparoscopic diagnoses. As it was suspected from the clinical view aged patients predominently suffered from posthepatic cirrhosis and from cirrhosis of unknown origin, from recurrent cholecystitis, obstructive jaundice, metastases and carcinosis of peritoneal cavity. Younger patients much more frequently showed toxic liver damage starting from fatty liver and ending up with fatty liver cirrhosis. Persistent acute hepatitis non associated with HBSAg was scarcely seen with the aged group. It was a frequent diagnosis with the younger control group. There are explanations given for the differing endoscopic results concerning aged persons and younger control persons.

Adult↗
PubMed1976

Time course and disposition of fucose radioactivity in rat hippocampus. A biochemical and microautoradiographic study.

Male adult rats were injected intraventricularly with L-[1-3H]fucose. At various intervals, ranging between 30 min and 11 days, one-half of the brain was prepared for microautoradiography, and the hippocampus from the other side was prepared for biochemical investigations. The TCA-precipitable proteins from the hippocampus homogenate were maximally labeled at between 8 and 24 h and remained at a high radioactivity level even 11 days after [3H]-fucose injection, the labeling being predominantly present in the solubilized insoluble proteins. Using gel electrophoretic separation, study of Tris-soluble material indicated a rapid turnover of soluble fucose-containing glycoproteins, whereas several slow-migrating bands of solubulized proteins revealed a time course suggesting the presence of fucose-containing glycoproteins with slower turnover rates. Using microautoradiography, a rapid labeling of neuronal cell bodies of the hippocampus was found, whereas the nuclei were not labeled. Perikarya were maximally labeled 4 h after [3H]fucose application. The radioactive material was continuously transported from the soma into the corresponding fiber layers, the latter being maximally labeled at a pulse interval of one day; even 10 days later a considerable amount of radioactivity could be detected in the neuropil.

Animals↗
PubMed1976

Increased fucose incorporation into rat hippocampus during learning. A biochemical and microautoradiographic study.

The incorporation of intraventricularly infected L-[1-3H]fucose into proteins of the hippocampus and visual cortex was studied during the acquisition of a shock-motivated brightness discrimination in rats. The labeling of Tris-soluble proteins from both regions was not significantly changed during learning, whereas solubilized insoluble proteins obtained from the hippocampus revealed an increased fucose incorporation in learned animals. A significant enhanced incorporation into some distinct, slow-moving, carbohydrate-rich protein bands, separated by polyacrylamide gel electrophoresis, was observed in material from CA1 and CA3 sectors as well as from area dentata of the hippocampus formation during acquisition. The corresponding gel bands from the visual cortex exhibited no differences between trained animals and controls. Jointly performed microautoradiography showed an increased fucose incorporation into most areas of the hippocampus in learned animals compared with active and passive controls. The most significant differences were found to occur mainly in substructures consisting of densely packed neuronal cells.

Animals↗
PubMed1976

Influence of uridine-5-monophosphate on 3H-leucine incorporation into hippocampal neurons during learning.

The influence of UMP (injected intraventricularly) on the incorporation of 3H-leucine (injected intraperitoneally) was studied in rats using the microautoradiographic technique. Under the conditions of a brightness discrimination, the incorporation of labeled leucine into hippocampal neurons was significantly increased by UMP pretreatment, whereas under control conditions UMP caused only a tendency of increase without any statistical significance. It is suggested, that under learning conditions UMP substitution increases RNA synthesis, which would yield an enhanced protein synthesis.

Animals↗
PubMed1976

Infrared dichroism of the DNA-caffeine complex. A new method for determination of the ligand orientation.

Infrared linear dichroism (LD) measurements on films of the DNA-caffeine complex in terms of the relative humidity (r.h.) show two main effects. Firstly, there is an insertion of caffeine molecules into the DNA double helix (B form), as evidenced by a very strong parallel LD behaviour of the 745 cm-1 band due to the C-H out-of-plane deformation vibration of caffeine. Furthermore, a high r.h. values a modified B form occurs in the complex similar to the B form recently reported by BRAHMS and coworkers for DNA-polypeptide complexes. The reversible B-A transition of the DNA in dependence of the r.h. is not affected in general in the presence of caffeine.

Animals↗
PubMed1975

Free and membrane-bound ribosomes and polysomes in hippocampal neurons during a learning experiment.

The ribosomes of the CA1 and CA3 pyramidal cells of hipocampus were investigated by morphometric methods after the acquisition of a shock-motivated brightness discrimination in rats. A significant increase in the total number of ribosomes was observed in CA1 cells of trained animals and in CA3 cells of both active controls and trained rats. A significant increase in membrane-bound ribosomes was obtained in CA1 and CA3 cells after training only. The results confirm the suggestion of an increased protein synthesis in hippocampal neurons during and after the acquisition of a brightness discrimination, as we have concluded from out previous investigations on the incorporation of labeled amino acids under identical experimental conditions. The results lead to the assumption that the protein synthesis in some neuronal cells may probably differ not only quantitatively, but also qualitatively in trained and untrained animals.

Animals↗