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Biomedical subjects

W Petek

Publications and source records attributed to W Petek.

At least 55 records · Page 3Linked to original sources
PubMed1977

[Low molecule and middle molecule metabolites with haemodynamic activity in the ultrafiltrate from uraemic patients (author's transl)].

Substances causing blood pressure changes and myocardial damage in rats were first detected in the ultrafiltrate obtained from patients on long-term haemodialysis by means of size separation, high-voltage electrophoresis and thin-layer chromatography. Of the 100 to 140 fractions produced by Sephadex G 15 chromatography at least 3 raised the blood pressure when administered in doses of 2 to 10 mg/0.1 to 0.2 ml 0.9% NaCl, that is the high molecular fraction 18 to 23 and the low molecular fractions 71 to 72 and 73 to 74. The fractions 96 to 98 and 97 to 106 showed high toxicity. Less than 1 mg/0.1 to 0.2 ccm 0.9% NaCl produced cardiac arrest. The fractions 35 to 55, which originate from the middle molecules and are retained in regularly uraemia, contain substances which caused a reduction in blood pressure at low dosage and cardiotoxic effects at a dosage of more than 10 mg. Thin-layer chromatography indicates that the substances most probably consist of peptides whose structure contains the amino acids leucine, isoleucine, valine, glutamic acid, aspartic acid, alanine and cystine.

Humans↗
PubMed1977

[Serum lipase activity and evocation test after endoscopic retrograde cholangio-pancreatography (author's transl)].

Serum lipase activity, which is a more specific indicator of pancreatic damage than serum amylase activity, was determined in 51 patients before and immediately after endoscopic retrograde cholangio-pancreatography (ERCP) and 24 hours subsequently. An increase in serum lipase activity without clinical signs of acute pancreatitis was observed immediately after pancreatography in 20 cases. Increased serum lipase activity after 24 hours is largely observed in patients with parenchymography or those displaying increased values already in the fasting state before ERCP. This observation emphasizes the need for caution in the assessment of the indication for ERCP in patients with preexisting increased serum lipase activity in view of the very real risk of acute pancreatitis and the need to avoid parenchymography of the pancreas. According to our experience the addition of a broadspectrum antibiotic such as gentamycin to the contrast medium may, to a large extent, avoid the development of acute pancreatitis. Lipase evocation tests carried out 10 days after the ERCP showed negative results in patients with increased enzyme activity and parenchymography after pancreatography. Hence, it is concluded that ERCP causes symptomless reversible biochemical pancreatic changes, as opposed to irreversible pancreatic damage.

Acute Disease↗
PubMed1977

[The importance of the serumlipaseevocationtest and the retrograde pancreaticography for the diagnosis of chronic pancreatic affections (author's transl)].

Comparative studies between activity of serumlipase, lipaseevocationstest and endoscopic retrograde cholangiopancreaticography (ERCP) in 45 patients with chronic pancreatitis, pancreatic cysts or pancreatic carcinoma confirm, that elevated enzyme values after caerulein stimulation indicate an alteration of the pancreatic parenchyma. However, this findings does not give information on the etiology of the damage. On the basis of the presented results it seems that the lipase-evocationstest has only limited value as screening method within the total of clinical methods for the assessment of light and medium severe stages of chronic pancreatitis and accompanying forms of pancreatitis. There is no evidence that this method may be used for screening for carcinoma of the pancreas. A positive result gives a valuable information, but the lack of an increase of lipase activity does not exclude the existence of pancreatic affection. In each case with clinical suspicion for pancreatic disease further diagnostic investigation by the ERCP and additional radiographic methods should be performed.

Adult↗
PubMed1976

Alteration of serum lipid values and lipoproteins by bacteria as a possible cause of artifacts in the screening of dyslipoproteinemias.

The intriguing observation of phospholipid deficiency in serum is described. The most prominent feature of sera was the completely abnormal lipoprotein electrophoretic pattern. The predominant occurrance of the phenomenon in the warm season suggested bacterial growth producing phospholipase C. B. cereus could be isolated from sera. In order to prevent these artifacts it is suggested to add 1 mg of Na2-EDTA to each ml of sample before dispatch.

Bacillus cereus↗
PubMed1976

[Changes of serum proteins after craniocerebral trauma - comparison of alpha 2 and alpha 2M (author's transl)].

Quantitive estimations of serum proteins, including Alpha 2M globulin, from 34 patients suffering from craniocerbral trauma have been carried out. The mean value curves over a period of two weeks following trauma are presented graphically. After severe injuries, depending on the size of the cerebral lesions, an increase in Alpha 2 of prognostic significance has been observed. Levels of Alpha 2M do not correlate directly with Alpha 2 globulin, and tend to fall initially. Falls in the first three days after injury may be prognostically significant from the point of view of the prediction of a lethal outcome. There are no significant changes in the levels of total protein, albumin, Beta globulin or Gamma globulin, but Alpha 1 globulin follows the curve of Alpha 2, although to a less marked degree. Correlations between serum protease activites and increases in Alpha 2 due to enzymatically active complexes are unconnected with Alpha 2M, a decrease in which indicates increased inhibitor activity and exhaustion of the inhibitor system.

Alpha-Globulins↗
PubMed1976

Serum globulin changes in patients with craniocerebral trauma.

Investigations of serum total protein, albumin, alpha-1, alpha-2, alpha-2M, beta- and gamma-globulin changes are reported in 48 patients with craniocerebral trauma. Only alpha-2 and alpha-2M globulins showed important variations, the first rising to three to four times normal values (112% on average) and directly correlating with the amount of tissue lesions. Alpha-2M changes were rather irregular with a tendency to severe decrease during the first days in patients with a bad prognosis. Reasons for non-regular changes of alpha-2M are discussed. Alpha-1 globulin showed a similar though less marked increase. Gamma globulin, on average, was diminished in the first week.

Beta-Globulins↗
PubMed1975

[Quantitative determination of aqueous humor proteins by electroimmodiffusion in an antiserum containing agarosegel (author's transl)].

An exact quantitation of aqueous humor proteins within a few hours can be attained by an modification of the quantitative estimation of the proteins by electrophoresis in agarose gel established by Laurell. The aqueous humor proteins form long immunoprecipitations in agargel. The length of the precipitats is proportional to the concentration. By determination of albumin, coeruloplasmin and transferrin the application can be pointed out.

Albumins↗