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Biomedical subjects

W N Scott

Publications and source records attributed to W N Scott.

At least 55 records · Page 3Linked to original sources

Zinc alpha-2 glycoprotein levels in serum and breast fluids: a potential marker of apocrine activity.

Zinc alpha-2 glycoprotein (ZnGP) was measured in human breast microcysts, breast secretions, breast cyst fluid and serum. Detectable amounts of ZnGP were found in all fluids but the highest levels were found in microcysts. Apocrine macrocysts had a higher ZnGP level than flattened macrocysts. In both cysts and secretions levels of ZnGP correlated with those of dehydroepiandrosterone sulphate. Levels were significantly higher in cyst fluids from women who developed further cysts during follow-up compared with those in fluid from women who did not. Concentrations of ZnGP in serum from breast cancer patients were significantly higher than controls but not women with breast cysts. Women with node positive breast cancer had higher serum levels compared with those in node negative patients. Women with more advanced breast cancer had higher serum ZnGP levels than those with earlier disease. ZnGP is a serum and breast marker of apocrine activity and may prove to be a useful prognostic marker in breast cancer.

Apocrine Glands↗

The mutagenic activity of human breast secretions.

Human breast secretions, as obtained by nipple aspiration, were analysed for the presence of mutagenic activity by means of the Ames test. The overall incidence of women with secretions giving positive tests against Salmonella typhimurium, either strain TA98 or TA100, was 7.64%. No significant association was identified between the incidence of mutagen-positive breast secretions and age, menopausal status or the presence of breast abnormality in these women. Furthermore, there was no obvious environmental characteristic common to women with mutagen-positive fluids.

Adult↗

Levels of androgen conjugates and oestrone sulphate in patients with breast cysts.

Plasma and cyst fluid were obtained from patients with palpable breast cysts and analysed for androgen conjugates and oestrone sulphate content by radioimmunoassay. Concentrations of androgen conjugates in cyst fluids varied from 15.6 to 475.5 mumols/l. These levels were much greater than those in plasma (1.3-5.2 mumols/l) and there was no association between values in cyst aspirates and plasmas obtained from the same individuals. Levels of oestrone sulphate in breast cyst fluids (1.5-744.0 nmol/l) were also generally in excess of those in plasma (2.0-59.9 nmol/l) and again no relationship was evident between concentrations in cyst fluid and the circulation. Neither was there a relationship between levels of androgen conjugate and oestrone sulphate in plasma. In contrast, a highly significant correlation (P less than 0.001) was identified between the androgen conjugate and oestrone sulphate content of cyst fluids. Levels of both androgen conjugates and oestrone sulphate were also significantly different in groups of cysts subdivided according to electrolyte classification, cysts with low Na+:K+ ratios having higher steroid concentrations than those with high Na+:K+ ratios. The biological significance of the relationship between the two conjugates in cyst fluids remains unclear but it is suggested that the accumulation of these steroids involves a common mechanism.

Androgens↗

Scintigraphic determination of patellar viability after excision of infrapatellar fat pad and/or lateral retinacular release in total knee arthroplasty.

A prospective study ascertained the effect of lateral retinacular release and/or excision of the infrapatellar fat pad on patellar vascularity after total knee arthroplasty. Fifty patients (70 knees) had postoperative technetium bone scans, which revealed a higher incidence of vascular compromise of the patella after lateral release than without lateral release. Excision of the infrapatellar fat pad, commonly performed to enhance exposure and simplify access to the proximal tibia, did not compromise patellar vascularity.

Adipose Tissue↗

Quantification of epidermal growth factor in human breast cyst fluids: correlation with dehydroepiandrosterone-sulphate and electrolyte concentrations.

Epidermal growth factor (EGF), dehydroepiandrosterone (DHA)-sulphate and [Na+] and [K+] were assayed in 78 cyst fluids from patients with a palpable breast cyst. Epidermal growth factor was detected in all but 2 cysts, the mean value +/- SEM being 506.2 +/- 39.3 ng/ml, with a range of 0-1,599 ng/ml. When the cyst fluids were sub-divided according to their [Na+]:[K+] ratio, group A cyst fluids ( [Na+]:[K+] less than 3) had a significantly higher (p less than 0.001) level of EGF than group B cyst fluids ([Na+]:[K+] greater than 3). Furthermore, the relationship between EGF and [Na+] and [K+] and between EGF and DHA-sulphate seemed to differ between the 2 cyst types and each cyst type was therefore analyzed separately.

Aged↗

Double-blind assessment of the value of magnetic resonance imaging in the diagnosis of anterior cruciate and meniscal lesions.

A prospective double-blind study was undertaken to evaluate the usefulness of magnetic resonance imaging in the accurate interpretation of pathological intra-articular changes in the knee. Forty-seven patients who were scheduled to have arthroscopy and three patients who wer to have arthrotomy volunteered for magnetic resonance imaging preoperatively. The radiologists had no clinical or roentgenographic information about the patients before the evaluation of the magnetic resonance images, and the radiologists' interpretations were unknown to the surgeon before the arthroscopy or arthrotomy was done. Our important observations were limited to the findings in the menisci and in the anterior cruciate ligament. Magnetic resonance imaging had a positive predictive value of 75 per cent, a negative predictive value of 90 per cent, a sensitivity of 83 per cent, and a specificity of 84 per cent for pathological findings in the menisci. For complete tears of the anterior cruciate ligament, the positive predictive value was 74 per cent; the negative predictive value, 70 per cent; the sensitivity, 61 per cent; and the specificity, 82 per cent. We believe that magnetic resonance imaging, when combined with clinical and roentgenographic examination, provides the most accurate non-invasive source of information that is currently available for pathological findings in the menisci and in the anterior cruciate ligament.

Adolescent↗

Management of selected problems in revision knee arthroplasty.

In discussing revision arthroplasty, understanding the failure mode is essential to success. In general there are five major categories that one must address in assessing the failed knee replacement: Infection, mechanical problems, extensor mechanism dysfunction, fractures adjacent to the components, and skin problems. These five areas are discussed in this article.

Biomechanical Phenomena↗

Rationale of the Knee Society clinical rating system.

A new total knee rating system has been developed by The Knee Society to provide an up-to-date more stringent evaluation form. The system is subdivided into a knee score that rates only the knee joint itself and a functional score that rates the patient's ability to walk and climb stairs. The dual rating system eliminates the problem of declining knee scores associated with patient infirmity.

Disability Evaluation↗

Lateral release and proximal realignment for patellar subluxation and dislocation. A long-term follow-up.

Fifty-two patients (sixty knees) who had a diagnosis of either patellar subluxation or dislocation had an operation consisting of a lateral release and proximal realignment of the patella. The duration of follow-up ranged from two to nine years. At the latest follow-up, forty-two (80.8 per cent) of the patients had a good or an excellent clinical result; only one patient had redislocation of the patella. The results were not affected by the grade of chondromalacia that was present at the time of operation; however, the differences in the results as correlated with the age and sex of the patient were statistically significant, the older patients having poorer results and the male patients, better results. Roentgenographic analysis of the postoperative position of the patella, as seen on the view described by Merchant et al., revealed that centralizing the patella in the intercondylar sulcus yielded the most favorable results.

Adolescent↗

Results after knee replacement with a posterior cruciate-substituting prosthesis.

From 1979 to 1984, eighty patients (119 knees) were arbitrarily selected for treatment with knee arthroplasty in which a posterior cruciate-substituting replacement was used. The average age of the forty-nine women and thirty-one men was 66.9 years (range, twenty-two to eighty-four years). Sixty-one right and fifty-eight left knees were operated on, and bilateral replacement was performed in thirty-nine of the eighty patients. The diagnosis was osteoarthritis in fifty-eight patients (eighty-eight knees), rheumatoid arthritis in fourteen patients (twenty-two knees), osteonecrosis in three patients (four knees), and traumatic arthritis secondary to a fracture of the tibia or femur in five patients (five knees). The average preoperative score on The Hospital for Special Surgery knee-rating scale was 47.5 points, and the average range of motion preoperatively was 88 degrees (range, 30 to 140 degrees). Of the 119 knees, eighty-seven had a varus alignment (maximum, 35 degrees) before knee replacement. After follow-up of two to eight years, the average score on The Hospital for Special Surgery scale was 90 points, and the average range of motion was 107 degrees. Of the 119 knees, 83 per cent were rated as excellent; 15 per cent, as good; none, as fair; and 2 per cent, as poor. Radiolucencies of one millimeter were present in 76 per cent of the knees; of two millimeters, in 7 per cent; and of three millimeters, in 3 per cent. No statistically significant correlation between radiolucencies and the clinical result was found. The results in knees of patients who had rheumatoid arthritis were not as good as those in knees of patients who had other diagnoses (F = 11.44). Our experience suggested that the posterior cruciate-substituting design provides more motion than do the cruciate-sacrificing surface-replacement designs, with no deleterious effects. The rate of infection (1.6 per cent) after these procedures, which were carried out in a standard operating theater with vertical airflow, was equivalent to that in other published series in which rooms with laminar airflow were used. Patients who had a bilateral procedure did as well as those who had a unilateral replacement, but they required approximately 3.5 more units of blood.

Adult↗

The relationship of lateral releases to patella viability in total knee arthroplasty.

A prospective clinical and radionuclide study was done to ascertain the effect of a lateral release on patella vascularity in total knee arthroplasty. Although the basic surgical procedure was the same in all 36 knees, postoperative technetium bone scans revealed a higher incidence (9 of 16, 56.4%) of vascular compromise in knees with a lateral release than in those without a lateral release (3 of 20, 15%). Although only one clinical complication (fractured patella not requiring secondary surgery) occurred, the lateral release itself appears to have a causal relationship with patella viability. It does not seem to be the sole determinant, however, because the development of a "cold" patella in three knees that did not have a lateral release implicates other factors, such as thermal necrosis and anatomic variation of the blood supply.

Aged↗

Insulin-stimulated sodium transport in toad urinary bladder.

Mammalian and teleost insulins increase active sodium transport by the toad urinary bladder at subnanomolar concentrations. This stimulation is evident within 15 min and persists for hours. Porcine proinsulin and a cross-linked derivative of bovine insulin are less effective than porcine insulin in stimulating the short-circuit current (SCC), indicating the specificity appropriate for activation of sodium transport through an insulin receptor. The initial stimulation by insulin of the SCC is not blocked by pretreatment with actinomycin D, puromycin, cycloheximide, or tunicamycin. However, in the presence of any one of these inhibitors the sustained increase in SCC is blocked and the rise is short-lived, lasting only 45 to 90 min. In amphotericin-treated bladders, the addition of insulin did not further stimulate SCC.

Amphotericin B↗

Posterior stabilized knee arthroplasty. Six years' experience.

The posterior stabilized knee arthroplasty, a descendent of the cruciate sacrificing total condylar design, is a cruciate-substituting prosthesis that achieves motion previously attainable only by cruciate-retaining knee arthroplasties. Its clinical success has not been associated with complications directly attributable to the design.

Adult↗

Natural history of cystic disease: the importance of cyst type.

All breast cysts aspirated from a series of 100 patients followed for a minimum period of 2 years were classified on the basis of electrolyte composition as apocrine or flattened, this being the nature of the epithelium lining the two populations of breast cysts. Patients with a single cyst were more than 3 times as likely to have a flattened rather than an apocrine cyst. Multiple cysts, whether simultaneous or sequential in any individual patient, were usually all of the same type, and were more commonly apocrine than flattened. A comparison of the frequency of subsequent cysts in patients whose initial cysts were of either apocrine or flattened type showed further cysts were over 5 times more common in patients who presented with apocrine cysts. These observations suggest that the natural history of cystic disease is closely related to cyst type.

Epithelium↗

A comparison of protein synthetic patterns of MDCK cells grown in serum and hormonally defined serum-free medium.

The development of hormonally defined serum-free media (HDM) for the culture of mammalian cells has allowed the study of specific cellular functions in a totally defined environment. Recently, several reports have indicated that there are differences in the basic cellular physiology of cells cultured in HDM when compared to cells cultured by the more traditional method of using serum-supplemented culture media (SM). We report here that there are significant changes in the protein synthetic pattern in MDCK cells grown in HDM. There were no changes exhibited during the first passage in HDM, but following 10 passages in HDM there was an increased isotope ratio of 1) plasma membrane proteins with molecular weights of 12,000, 36,000 and 68,000 and 2) endoplasmic reticulum proteins with molecular weights of 12,000 and 37,000. Additionally, the incorporation of methionine and uridine were significantly increased in cells cultured in HDM for 10 passages. At present, we believe that these changes in the protein synthetic patterns are due at least partially to increased protein synthesis as indicated by monosome/polysome ratios. Therefore, though the use of HDM offers a completely defined system for studying cellular function, results obtained using HDM must be interpreted with caution when comparing them to previous studies that have used SM.

Animals↗