Inhibition of glucose and tolbutamide-induced insulin release by iodoacetate and antimycin A.
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Biomedical subjects
Publications and source records attributed to W M Kirsch.
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Intact lyophilized nuclei are obtainable from a variety of tissues, either in situ or in culture, by freezing at -156 degrees C, drying at -25 degrees C, and mechanical disassociation in glycerol at 2 degrees C. Centrifugal separation of nuclei is accomplished in an 85 : 15 by volume mixture of glycerol and 3-chloro-1,2 propanediol at 2 degrees C. The method gives homogeneous nuclear preparations in high yield with preservation of labile and water-soluble constituents.
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No correlation was found between the cellular steady-state concentrations of glucose-6-phosphate, 6-phosphogluconate, and reduced nicotinamide dinucleotide phosphate and resistance versus sensitivity to catabolite repression.
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