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Biomedical subjects

W Lasek

Publications and source records attributed to W Lasek.

12 recordsLinked to original sources
PubMed1992

Two patterns of NK activity changes following blood donation: decrease in the beginners and restoration in regular blood bank donors.

NK cell activity of peripheral blood lymphocytes was determined in voluntary blood bank donors in a standard 4-hr 51Cr-release cytotoxicity assay. When blood donors were divided into groups according to the total amount of blood they had donated in the past, decreased NK activity was found in "moderate" donors who had donated between 3 and 9 l of blood, but not in those who had donated < or = 3 or more than 9 l of blood before testing. This observation was the rationale for a study on the effects of regular blood donations on NK activity in randomly selected voluntary blood bank donors re-tested over a period of time. The study demonstrated decreased NK activity in the second measurement in donors who had donated up to 6 l of blood before the study, and an increase in NK activity between the first and the second testing in those who had donated more than 6 l of blood. This result, together with data obtained at the population level, suggests that some compensatory mechanism(s) regulate NK activity in the course of regular blood donation.

Adult↗
PubMed1991

Evidence that liposome incorporation of cyclosporine reduces its toxicity and potentiates its ability to prolong survival of cardiac allografts in mice.

Using liposomes, multilamellar lipid vesicles (MLV), we have found that liposome-incorporated cyclosporine is not only less toxic but also more effective in prolonging survival of cardiac allografts in mice. Following intravenous injection of liposome-incorporated drug, cyclosporine levels in blood were shown to decrease rapidly, while concentrations in spleen were higher (when compared with concentration following administration of commercial preparation). In this context, possible mechanisms of the beneficial effect of liposome-incorporated cyclosporine are discussed.

Animals↗
PubMed1989

The stimulation of EL-4 cells to produce interleukin-2 and its potential use in immunocytotoxicity testing.

The ability of EL-4 thymoma cells to produce interleukin-2 (IL-2) following exposure to phorbol-12-myristate 13-acetate (PMA) and Concanavalin A (Con A) has been studied in vitro using medium containing either 10% or 1% fetal calf serum (FCS). The potent stimulatory effect of PMA on IL-2 production by EL-4 cells has been confirmed by measuring 3H-thymidine incorporation by the IL-2-dependent T cell line, CTLL-2, in the presence of conditioned medium (CM) from stimulated cultures. EL-4 cells produced several times more IL-2 when cultured in medium containing 10% FCS than when only 1% FCS was present. Added together, PMA and Con A acted synergistically in some EL-4 cell cultures. The ability of E:-4 cells to produce IL-2 was maintained after further incubation without stimulants. CM with IL-2 activity from stimulated EL-4 cells could prove useful in immunotoxicity testing.

Animals↗
PubMed1989

Opposite effect of donor-specific immunization by blood or splenocytes on the survival of heart tissue transplants in strong versus weak histoincompatible combination in mice.

Recipients of allogeneic heart tissue were pretreated intravenously with blood or injected intraperitoneally with spleen cells. In H-2 incompatible donor-recipient combination (BALB/c----CBA/H) donor-specific immunization, both with blood and spleen cells, resulted in acceleration of graft rejection. On the other hand, increase in graft survival was observed following donor-specific but not third-party blood pretreatment in mice compatible in H-2 complex but bearing different minor histoincompatible antigens (BALB/c----DBA/2).

Animals↗
PubMed1989

The influence of ABO blood groups, Rh antigens and cigarette smoking on the level of NK activity in normal population.

In this study we have examined the influence of ABO and Rh blood groups as well as the effect of cigarette smoking on the level of NK activity in healthy men. Blood bank donors and age-matched non-donors have been evaluated independently. As far as ABO blood groups are concerned, higher NK activity in individuals with AB blood group in comparison with those with group A and those with groups A, B, and O taken together have been demonstrated. No effect of Rh antigens on NK activity has been found. Similarly, there was no influence of cigarette smoking on NK activity. Consistently lower values of NK activity in blood bank donors in comparison with non-donors have been shown.

ABO Blood-Group System↗
PubMed1979

[Aneurysmal bone cyst (author's transl)].

Aneurysmal bone cyst in two patients are described and some radiological and clinical problems discussed, especially some difficulties in establishing the X-ray diagnosis of the infrequent disease.

Adolescent↗