Biomedical subjects
W Löhr
Publications and source records attributed to W Löhr.
[Leptilan. Dissolution, bioavailability and first therapeutic results in children with different epilepsy forms].
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[The methodologic basis of azure B-eosin stain--demonstration of its effectiveness in examples of hematologic practice in comparison to the heretofore customary May-Grunwald-Giemsa stain].
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[Can azur-B eosin replace the May-Grünwald-Giemsa stain?].
A new method is described for staining blood and bone marrow smears. It is characterized by the presence of only two dyes, purified azure B and eosin in methanol, as stock solutions. Staining results are equivalent to those obtained by using the traditional dye mixtures according to May and Grunwald, Giemsa, Leishman or Wright. Different from these azure B-eosin staining can be standardized and is easier to be handled. Correlations between the azure-B-eosin and May-Grunwald-Giemsa (MGG) staining methods are briefly discussed.
The azure dyes: their purification and physicochemical properties. II. Purification of azure B.
A method is described for the purification of the dye azure B in quantities sufficient for biological staining experiments on a larger scale. The method is based on the use of column chromatography. Two columns are employed. In column A with silica gel as adsorbent the azure B fraction is isolated from a suitable substrate ('technical' azure B gained by a modification of Bernthsen's synthesis of methylene blue, or polychrome methylene blue) using an acetate-formate mixture as eluent. In column B, on an Amberlite polymeric adsorbent (XAD-2) the acetate-formate anions are exchanged in chloride. Regeneration of both columns is possible: KMnO4, Na2S2O4 and water are run through column A; 5% NaOH, methanol and water through column B. Purification of azure B on economic terms is thus attained. The opinion is expressed that this method is also applicable to the purification of other cationic dyes.
The azure dyes: their purification and physicochemical properties. I. Purification of azure A.
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[Vital staining with phenothiazine derivatives. Comparative application of commercial and purified dyes].
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[9. Problems of column chromatographic and thin layer chromatographic purification of dyes, demonstrated in the example of brilliant cresyl blue].
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[Problems of column and thin layer chromatographic purification of dyes, demonstrated with brilliant cresyl blue as an example].
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