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Biomedical subjects

W L Bayer

Publications and source records attributed to W L Bayer.

At least 37 records · Page 2Linked to original sources
PubMed1980

The use of blood and blood components in 1,769 patients undergoing open-heart surgery.

There has been a decrease in the use of whole blood and red cell transfusions during and after open-heart operations in the greater Kansas City area from an average of slightly more than 9 units per patient from 1969 through 1971, to just over 3 units per patient from 1975 through 1977. In 1977, 1,256 patients, or 71% of 1,769 patients, underwent coronary artery bypass exclusively and had an average transfusion utilization of 2.6 units. All other open-heart operations averaged 4.7 units per patient. Hemodilution and the acceptance of hematocrits between 25 and 30% in open-heart operations are probably the main factors responsible for lower transfusion use per patient, while the increased proportion of patients undergoing coronary artery bypass accounts for a further decrease in the average amount of blood used per patient. It is of note that blood transfused to patients having an open-heart operation was not significantly fresher than blood for routine use, yet hemostasis was not a problem as evidenced by the small use of fresh-frozen plasma in 67 patients (3.8%) and platelet concentrates in 42 patients (2.4%).

Adult↗
PubMed1980

Characterization of human erythrocyte alloantibodies by IgG subclass and monocyte interaction.

Human blood group antibodies of 11 specificities were examined for ability to mediate interaction with normal blood monocytes, IgG subclass composition, and titer score. Most antibodies were normally non-complement-binding (except anti-JKa) and clinically significant (except anti-Lub). A simple and relatively rapid in-vitro assay for the visualization of sensitized erythrocyte-monocyte interaction is described. Capillary procedures were adapted for IgG subclass composition determinations. Standard serologic tests were used to assess antibody titer score. A relationship appeared to exist between increased antibody titer score and increased interaction of sensitized erythrocytes (RBC) with normal blood monocytes (MNL), insofar as the mean titer score for antibodies that did mediate significant interaction was 52, whereas titer scores for antibodies that did not averaged 25. One or more examples within all antibody specificity, mediated significant RBC-MNL interaction. No relationship was observed between IgG subclass composition and mediation of RBC-MNL interaction except that all antibodies that did mediate interaction contained IgG1 or IgG3. Characteristic patterns of IgG subclass composition were observed for many antibody specificity groups, although too few examples were tested for valid statistical analysis. This study identifies two features of common blood group antibody populations that are instrumental in mediating erythrocyte destruction by monocytes in vitro. Similar information may be of value in predicting the result of erythrocytes transfused to patients with antibodies of unknown clinical significance.

Cell Adhesion↗
PubMed1979

Antigens of human cytomegalovirus: electroimmunodiffusion assay and comparison among strains.

The antigens of strain AD169 of human cytomegalovirus (CMV) were extracted by various methods and at different times following the appearance of cytopathic effects (c.p.e.) in infected fibroblasts. Assay with a pooled human serum in electroimmunodiffusion (EID) revealed that the most reactive preparations were obtained by shell-freeze (SF) extraction on the fourth day after 4+c.p.e. As many as 20 antigens could be detected in the original gels, most of which were stable upon storage at 4 degrees C for up to 4 weeks; of these, about 14 can be reproducibly seen on photographs. EID runs on day 4 SF preparations from high-passage CMV strains C87 and Davis and low passage recent isolates VD14, 1694 and 1723 resolved, respectively, 15, 15, 13, 11 and 11 antigens in the original gels (11, 9, 11,8 and 9 are visible in photographs). Strains 1694 and 1723 shared fewer antigens with one another and with high passage strains than were shared among the latter, whereas VD14 had relatively large numbers of antigens common to both low and high passage strains. At least six antigens were common to all strains.

Antigens, Viral↗
PubMed1975

A serum haemagglutinating property dependent upon polycarboxyl groups.

A serum agglutinin reactive with red cells in the presence of polycarboxyl groups is reported. It is likely that this represents an additional example of the type of agglutinin previously described as agglutinating red cells in the absence of ionized calcium. Experimental evidence is presented indicating that it is free polycarboxyl groups that potentiate agglutination and that any metal ion, such as calcium, capable of chelating with these groups will prove to be inhibitory.

ABO Blood-Group System↗
PubMed1975

Cytomegalovirus infection in a volunteer blood donor population.

Among 223 volunteer blood donors who were studied for evidence of cytomegalovirus (CMV) infection, 58 percent had complement-fixing antibody and 59 percent had indirect hemagglutinating antibody to CMV. No virus was isolated from any donor's washed leukocytes or leukocyte-rich plasma in fibroblast monolayer culture. In seven asymptomatic donors (3 percent), CMV was recovered from urine cultures obtained at the time of blood donation. However, at the time of reexamination, viruria was no longer present and serum antibody titers had not changed. In the three patients studied who received blood from three of the cytomegaloviruric donors, serological evidence of CMV infection developed (fourfold or greater indirect hemagglutinating antibody rise), and one recipient also developed cytomegaloviruria; no illnesses was associated with these infections. Further study is needed to establish that the detection of viruria in donors may identify potentially infective blood.

Antibodies, Viral↗
PubMed1975

Plasma exchange with plasma protein fraction and lactated Ringer's solution using the continuous flow cell separator.

Ten patients underwent 50-70 percent plasma exchanges using the continuous flow cell separator. The exchange material consisted of Plasma Protein Fraction (PPF) and Lactated Ringer's Solution (LRS) instead of fresh frozen, modified or lyophilized plasma. No bleeding or other complications were encountered. The coagulation factor activity after exchange was above that required for hemostasis. The procedure is safe, expeditious and efficient.

Blood Proteins↗
PubMed1969

Uremic bleeding.

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Blood Coagulation Disorders↗