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Biomedical subjects

W Kraus

Publications and source records attributed to W Kraus.

At least 91 records · Page 5Linked to original sources
PubMed1983

[Microangiographic examinations of different human tumors after transplantation to thymus aplastic nude mice].

Ten different human tumors (two soft tissue sarcomas, seven adenocarcinomas of the colon and stomach and, one squamous cell carcinoma of the lung) were implanted s.c. in thymus-aplastic mice and retransplanted over several generations. In 40 animals, the tumors were studied by microangiography and microscopy and classified according to angiographic tumor criteria. Tumor transplants always revealed similar angiographic patterns. The microscopic results presented a good correlation with the roentgenologic findings. The microstructure of the tumor vessels and lacunes are analyzed and discussed. The nude mice model is well suitable for the research of human tumors.

Angiography↗
PubMed1983

Purification and characterization of two new soluble placental tissue proteins (PP13 and PP17).

Two new soluble placental tissue proteins (PP13 and PP17) have been isolated and characterized. PP13 has an electrophoretic mobility the same as that of albumin, an isoelectric point in the range 4.7-4.8 and a sedimentation coefficient of 3.1 S. Its molecular weight was found to be 30 000. PP13 appears to be composed of two identical subunits which are held together by disulfide bonds. PP17 has an electrophoretic mobility in between the beta 1- and alpha 2-globulins, an isoelectric point in the range 5.2-5.3 and a sedimentation coefficient of 2.7 S. Its molecular weight was determined to be 30 300 by ultracentrifugation and 38 000 by SDS-polyacrylamide gel electrophoresis. PP17 apparently consists of a single peptide chain. The amino acid and carbohydrate compositions of these proteins also have been determined. Immunochemical methods were used to detect and quantitate the new proteins in extracts of placental and other human tissues as well as in body fluids. From one human term placenta an average of 3.7 mg PP13 and 2.5 mg PP17 could be extracted. In concentrated extracts of other human tissues and in body fluids, these proteins could not be detected, at least not in concentrations higher than 1 mg/dl. The immunohistochemical localization of these proteins as well as measurement of their concentrations in body fluids by sensitive radioimmunoassays are presently under investigation.

Amino Acids↗
PubMed1982

[Megaloblastic maturation disorder of erythropoiesis in plasmacytoma and chronic lymphatic leukemia].

Quantitative and qualitative investigations of the erythropoiesis in the sternal marrow of untreated 52 patients with a plasmacytoma and 46 patients with a chronic lymphatic leukaemia resulted in about half the cases in an indicated or more distinct megaloblastic disturbance of maturation in a part of the erythroblasts. These changes of the erythroblastic cells might be the expression of a neoplastic transformation and of a disturbance of the differentiation of the pluripotent stem cells in these diseases.

Aged↗
PubMed1982

[Experimentally induced tumors used for angiographic estimation of embolisation and cytostatic treatment (author's transl)].

In 12 rats tumors have been induced chemically by intraperitoneal application of dimethylnitrosamine. This method is simple and reliable and the effect of tumor embolisation can be followed easily. Thymus aplastic nude mice with transplanted human tumors deserve strict care. Tumor microangiograms of 48 animals demonstrate a close similarity with angiograms of corresponding human tumors. The vascular pattern does not alterate after several transplantations, after cytostatic therapy a slight hypervascularisation develops.

Angiography↗
PubMed1982

[Problem of the early diagnosis of Goodpasture syndrome].

It is reported on a 16-year-old patient with Goodpasture's syndrome in whom in a period of two years recurrent respiratory infection with haemoptyses appeared. Shortly after admission to hospital he died of respiratory insufficiency. Immunohistologically post mortem on pulmonary and renal tissue could be proved diffuse linear deposits of IgG and C3 on the basement membrane of the renal glomeruli and the alveoli. Histologically prevailed pulmonary haemorrhages and pulmonary haemosiderosis, whereas on the kidneys only a focal segmental mesangioproliferative glomerulonephritis was present. References are given to the possibility of early diagnosis by means of immunohistological examinations on the lungs and/or biopsies of the kidney and the determination of circulating glomerular antibodies of the basement membrane, since despite general knowledge also in adequate anamnesis with relapsing pulmonary haemorrhages at juvenile age it is not always thought of this fact and hopeful courses under early therapy with corticoids, immunosuppressive drugs and perhaps plasmapheresis are described in literature.

Adolescent↗
PubMed1981

[Megaloblastic erythropoiesis in stem cell leukemias].

Among 62 untreated patients with acute leucaemia the primary appearance of a megaloblastic erythropoiesis in the sternal marrow was found in 21 patients. Age and sex were without importance. Megaloblastic cell changes were found only in those patients, the leucaemia cells of whom were classified as particularly immature and were attributed to the type of stem cells or paraleucoblasts. The megaloblastic erythropoiesis might be an expression of a particularly severe neoplastic change and disturbance of differentiation of the pluripotent stem cell. The behaviour of the primarily already megaloblastically disturbed erythropoiesis in patients with leucaemia under cytostatic therapy is described on two observations of the course, in which cases the change of the findings in the bone-marrow is evident.

Adult↗
PubMed1980

[Chest x-ray and hematocrit value in acute myocardial infarction].

The possibility of recognizing a cardiac insufficiency by repeated thoracic roentgenograms at the bedside and the behaviour of the haematocrit value were investigated on 210 patients with infarction in the early phase. 56% of the patients with infarctions had roentgenological signs of a pulmonary stasis. Only half of these patients showed clinical signs of cardiac insufficiency. In 66% of the patients with infarctions increased haematocrit values were found, 19% of them had values of 60% and higher. Younger and male patients with infarctions had more frequently increased haematocrit values. In hypertension, diabetes, hyperlipidaemia, hyperuricaemia and adiposity patients with infarctions had increased haematocrit values in about 70% of the cases. The group of patients treated with as well as without diuretics showed 2/3 of the cases decreasing or remaining and in 1/3 of the cases increasing haemotocrit values.

Age Factors↗
PubMed1980

[New placental protein (PP15) with immunosuppressive properties (author's transl)].

The isolation and characterization of a new placental protein (PP15) which has immunosuppressive properties are described. The protein was purified from extracts of human term placentae by rivanol and ammonium sulfate fractionation, gel filtration, ion exchange chromatography, preparative zone electrophoresis, and chromatography on hydroxyapatite. PP15 was found to have a sedimentation coèfficient of 2.9 S and a molecular weight of 30,700 daltons as determined by ultracentrifugation; its molecules apparently are composed of two identical subunits which are held together by non-covalent bonds. The electrophorectic mobility of PP15 corresponds to that of albumin. PP15 is a glycoprotein and contains 3.3% carbohydrates (hexoses 2.8%, hexosamines 0.3%, sialic acid 0.2%). The amino acid composition of this protein was also determined: the most abundant amino acids in the peptide chain were found to be glutamic acid, aspartic acid, isoleucine, and leucine. PP15 has immunosuprressive properties: on testing its effect on the lymphoycte transformation in the MLC-test in vitro a significant inhibitory activity could be demonstrated.

Aspartic Acid↗
PubMed1979

[Isolation and characterization of a new placenta specific protein (PP10) (author's transl)].

PP10 was isolated from aqueous extracts of human term placentae by fractionating the proteins with rivanol and ammonium sulfate, by gelfiltration on Sephadex G-150 and by use of immunoadsorbents. PP10 apparently is a protein specific for the placenta; it could not be detected in extracts from other human tissues. From one human term placenta an average amount of 20 mg PP10 can be extracted. In sera from pregnant women PP10 is usually present only in trace amounts (less than 0.1 mg/100 ml). PP10 has the electrophoretic mobility of an alpha1-globulin and an isoelectric point of 5.1. The purified protein sediments with 3.8 S. PP10 was found to have a molecular weight of 48,000 as determined by ultracentrifugation and a molecular weight of 65,000 as determined by SDS-PAA gel electrophoresis. PP10 is a glycoprotein containing 6.65% carbohydrates (hexoses 4.8%, hexosamines 1.2%, fucose 0.05%, sialic acid 0.6%). The amino acid composition of PP10 has been determined, too; the most abundant amino acids in this protein are glutamic acid, aspartic acid, leucine and alanine.

Electrophoresis↗
PubMed1977

[Isolation and characterization of pregnancy-specific beta1-Glycoprotein from urine of pregnant women (author's transl)].

With use of the immunoadsorption technique the protein antigenically related to pregnancy-specific beta1-glycoprotein (SP1), was isolated from urine of pregnant women. The physical and chemical properties of this protein were determined and compared with those of SP1. The antigen isolated from urine has a smaller molecular weight and is immunochemically only partly identical with SP1 found in pregnancy serum respectively in the placenta. The protein occuring in urine seems to be a split product of SP1 and is supposedly formed by an enzymatic degradation of the native proteins. The concentration of the protein in urine of pregnant women throughout gravidity was quantitated immunochemically using a modified Laurell-technique. During the first and second trimester of pregnancy values ranged from 0,0-0,4 mg/100 ml. In the last trimester there was an increased in the concentration of urine-SP1 with values ranging from 0,0-3,0 mg/100 ml and a mean concentration of about 1 mg/100 ml.

Female↗
PubMed1976

[Isolation and characterization of the placental proteil pp1 (author's transl)].

The purification and characterization of the placental protein PP1 is described. The protein has been isolated from an aqueous extract of human term placentae using salt and ethanol fractionation procedures, gel filtration, preparative zone electrophoresis and chromatography on hydroxylapatite. PP1 sediments with 8.4 S and has a molecular weight of 160,000 daltons; it is composed of apparently four identical subunits, which have a molecular weight of 40,000 +/- 2000 daltons and are held together by non-covalent bonds. PP1 is a glycoprotein with a carbohydrate content of 2.7% and has the electrophoretic mobility of an alpha1-globulin. Immunochemical methods were used for the detection and quantitative determination of the protein. PP1 is not found in normal plasma or in erythrocytes; neither could it be detected in sera from pregnant women nor in sera from patients suffering from malignant diseases. PP1 is a tissue protein, but not specific for the placenta; it is also found in other human tissues. The average amount of PP1 extracted from one human term placenta was determined to be around 3 mg. Immunogluorescent studies revealed that the protein is located in the cytoplasma of the syncytium as well as in the stroma of the villi.

Cytoplasm↗
PubMed1976

Dosimetry in single lung cells by means of microautoradiographic activity measurements.

After inhalation of compounds containing promethium-147 in the lungs of mice most of the activity is deposited in the form of local concentrations (hotspots). By means of a special quantitative microautoradiographic method using stripping film ORWO K 105, measurements of the activity of single hotspots of about 10(-14) Ci are possible. A microphotometer with a variable measuring diaphragm is used for the determination of the density profile of the autoradiographic image in order to get hotspot depth within the biological specimen. To determine hotspot activity it is necessary to calibrate the film with a Pm-147 plane source. The systematic and random errors of the method are discussed in detail, giving a total error of +/- 21% (SD) for one hotspot activity measurement. A few examples of biological results obtained by the method are given. Simple models are used to calculate doses absorbed in macrophage and alveolar cell nuclei from the measured activities.

Animals↗