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Biomedical subjects

W Klein

Publications and source records attributed to W Klein.

570 records · Page 32Linked to original sources
PubMedDate not supplied

Subclinical thyroid disorders in patients with dilated cardiomyopathy.

UNLABELLED: Severe thyrotoxicosis can cause irreversible congestive heart failure. To investigate the coincidence of subclinical thyroid disorders and idiopathic dilated cardiomyopathy (IDC) we investigated these patients with respect to their morphological and functional thyroid status. Thyroid sonography as well as thyroid hormone levels were measured in all patients. RESULTS: Sixty-one patients (50 male, 11 female) with chronic stable IDC were included. Two out of 61 patients showed completely normal thyroid morphology and function. The other 59 patients showed either morphological or functional abnormalities or both. Of the 53 patients with morphological abnormalities 23 patients (all male) showed diffuse goiter as opposed to 29 nodular enlarged organs (24 male, 5 female). No clinically significant hypothyroidism or thyrotoxicosis was seen. A good correlation was found between the duration of IDC and thyroid volume (r = 0.44; p < 0.001). Two patients died during the study period, 1 from sudden death and 1 from progressive heart failure. CONCLUSION: Subclinical thyroid disorders are frequently seen in patients with long-standing IDC when they live in an area of chronic iodine deficiency. This can be explained by chronic salt restriction as basic treatment for congestive heart failure. Therefore we conclude that examination of the thyroid gland should be done routinely in patients with IDC, especially when restriction of salt intake is recommended by the treating physician.

Adult↗
PubMedDate not supplied

The use of the Ma-Griffith technique for percutaneous repair of fresh ruptured tendo Achillis.

All patients treated for a rupture of the Achilles tendon by the technique of percutaneous repair were identified from a five year period up to 1987. A review of the patients and results of this technique was undertaken using a clinical grading system. Review was conducted between 18 and 81 months following the injury. Of 38 patients followed up, 71% achieved good results and only 8% poor results. The re-rupture rate for this series was 8%. Sural nerve involvement (13%) was found to be a troublesome complication. During the period under study, a modification to the technique was introduced which abolished this complication. We present our results and describe the modification to the technique. The importance of the use of nonabsorbable sutures is emphasized.

Achilles Tendon↗
PubMedDate not supplied

[Effect of sodium fluoride on DNA metabolism during therapy for osteoporosis].

The influence of sodium fluoride therapy on semiconservative DNA-synthesis and DNA-excision repair was investigated in lymphocytes of the peripheral blood of 6 patients with osteoporosis. The daily dose of sodium fluoride was 25 mg in the first week, and subsequent 50 mg during a period of 15 to 37 weeks. No significant effect on the tested parameters was found in 5 subjects. One patient showed significantly inhibited DNA-repair in the lymphocytes up to the ninth week, but during subsequent therapy normal values were obtained in this patient also. In conclusion sodium fluoride seems to have no effect on DNA-metabolism in lymphocytes of patients with osteoporosis.

DNA↗
PubMedDate not supplied

[Mode of action of D-penicillamine in chronic polyarthritis. 2. Studies on DNA repair in synovial fluid cells following intra-articular administration of D-penicillamine].

6 patients with rheumatoid arthritis were treated intraarticularly with 50 or 100 mg D-Penicillamine. The influence of this substance on DNA repair in cells of synovial fluid was investigated. DNA repair was investigated by gradient centrifugation. 50 mg D-PA showed no effect, 100 mg D-PA a slight slowing of the DNA-rejoining till the native supercoiled DNA form.

Adult↗
PubMedDate not supplied

Cathepsin B in tumors, normal tissue and isolated cells from the human lung.

Human lung tumors of different histologic cell types and adjacent normal lung parenchyma, purified lung parenchyma, purified lung macrophages and three human lung-derived cell lines were investigated in an attempt to identify tumor specific premature and mature cathepsin B species. By polyacrylamide gel electrophoresis and immunoblotting techniques with specific antibodies we detected mature cathepsin B forms with molecular masses of 31 kDa and 32 kDa in tissues. Reductive conditions revealed in these populations single and double chain 31/32 kDa forms. The latter were recognized by their heavy part, the 26/27 kDa molecule forms. Qualitative differences in the pattern of cathepsin B species between tumor and corresponding normal material or between different histologies of lung tumors were not found. However, tumor material is considerably richer in cathepsin B activity than normal material. Isolated alveolar macrophage populations and established cell lines showed the same cathepsin B pattern as tissues. All these cells released cathepsin B proforms of 44 to 46 kDa into the culture medium, indicating that the release of pro-cathepsin B cannot be considered a tumor-specific mechanism. The secreted proforms were sensitive to in vitro activation by pepsin.

Cathepsin B↗
PubMedDate not supplied

Charge polymorphism in human lung cell pro-cathepsin B.

Secreted pro-cathepsin B of HS-24 human lung-tumour cells, human alveolar macrophages and Wi-38 human lung-fibroblast cells was pre-purified by ion exchange chromatography and investigated by 2D gel electrophoresis. Four (Wi-38), six (HS-24) and ten (alveolar macrophages) polypeptides differing in charge, but with the same molecular mass of 45 kDa, were found. The isoelectrical points of these polypeptides ranged from 5.43 to 6.57. Deglycosylation reduced the mass (7 kDa) but did not change the charge pattern. This investigation established cell type-specific patterns of secreted pro-cathepsin B-forms, but only parts of these may be cell type-specific forms depending on differentiated expression of mRNA and post-translational modification.

Carcinoma, Squamous Cell↗
PubMedDate not supplied

Microscopic comparison of the synovial changes in rheumatoid arthritis and ostroarthritis.

Whereas the histopathologic picture of the synovial membrane in rheumatoid arthritis varies widely and that in osteoarthritis displays a small range of changes, light- and transmission electron microscopic studies of synovial sections from both disease entities complement each other and assist reciprocally to corroborate the observed changes. Beyond that comparative survey of the ascertained histopathologic features and their correlation with clinical observations disclose that a study of a larger material of specimens permits with some limitations to infer the nature of the joint disease.

Arthritis, Rheumatoid↗