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Biomedical subjects

W Keil

Publications and source records attributed to W Keil.

69 records · Page 4Linked to original sources
PubMed1977

The topography of porcine lactate dehydrogenase isoenzyme H4. The identification of lysines on the surface.

Porcine lactate dehydrogenase isoenzyme H4 was treated with methyl 6-(2,4-dinitrophenylamino)hexanimidate hydrochloride and the lysines modified hereby were identified. For this purpose 20 chymotryptic-tryptic N epsilon-[6-(2,4-dinitrophenylamino)hexanimidoyl]lysine containing peptides were isolated by means of gel chromatography, countercurrent distribution, thin-layer chromatography and ion-exchange chromatography. Their amino acid composition, the amino end groups and their electrophoretic mobilities were determined. With these data, the known primary structure of the procine lactate dehydrogenase isoenzyme H4 and the 6-A resolution structure analysis performed by Rossmann et al.[1] we identified the following lysines on the surface of the quarternary structure of the enzyme: no. 4, 6, 60, 77, 82, 121, 157, 179, 226, 230, 241, 306, 308, 316, 327 and 330. No modified lysine peptides were found in the intersubunit binding sites.

Amino Acid Sequence↗
PubMedDate not supplied

Recombinant human Fab antibody fragments to HIV-1 Rev and Tat regulatory proteins: direct selection from a combinatorial phage display library.

A human Fab phage display library has been produced from peripheral blood lymphocytes of an individual who was asymptomatic after 10 years of infection with human immunodeficiency virus type-1 (HIV-1). The library was panned against the HIV-1 Rev and Tat regulatory proteins and several clones, producing Fab binding to these proteins, were isolated (3 to Rev and 4 to Tat) with binding constants varying from 10(-6)M to 10(-8)M. DNA sequencing demonstrated two unique anti-Rev Fab clones, but the four anti-Tat Fab comprised only two unique IgG1 heavy chain Fd fragments, illustrating redundancy of light chains. Peptide mapping of the epitopes recognized by these Fab indicated that three of the anti-Tat Fab were directed to the functional domain between amino acid residues 22-33 of the Tat molecule, and that binding was inhibited by reduction of this cysteine-rich region with dithiothreitol. The anti-Rev Fab were directed to sites adjacent to the Rev basic nucleolar localization sequence (residues 52-64) and to the Rev activation domain (residues 75-88). Binding constants were of a similar order to that of an anti-Rev single-chain Fv fragment (SFv) used successfully for intracellular immunization, and as such intracellular effects with the human anti-Tat and anti-Rev Fab are not precluded. These newly described human antibody fragments to HIV-1 regulatory proteins may be critical moieties for gene therapeutic protocols, to control HIV-1 replication in human cells.

Amino Acid Sequence↗
PubMedDate not supplied

The diagnostic significance of haemorrhage of the posterior cricoarytenoid muscles.

A number of authors have indicated that haemorrhage of the posterior cricoarytenoid (PCA) muscles can be used as a diagnostic criterion to establish the cause of death. Other authors even consider it to be 'forensic evidence' when differentiating between natural and violent death. They agree on agonal asphyxia or dyspnoea as a developmental mechanism, possibly in combination with a 'convulsive' dilatation of the glottic cleft. In view of this difference of opinion, we conducted a study of our own. Retrospective evaluation of 2060 post-mortem examinations performed in 1996 at the Institute of Forensic Medicine of the University of Munich disclosed 28 cases demonstrating visible haemorrhage of the PCA muscles. The study revealed no correlation between these findings and certain causes of death, nor any indication of certain mechanisms of development.

Adolescent↗
PubMedDate not supplied

[Demonstration of the loosening of hip endoprostheses by computerized tomography].

A pilotstudy was undertaken to evaluate the utilisation of modified computed tomography as an early diagnostic means to confirm the loosening of the total hip endoprosthesis. In nine of the twelve cases the CT investigation clearly indicated the loosening of the endoprosthesis. With respect to the remaining three patients, no conclusions could be drawn because of the presence of artifacts. It is of further interest to note pertaining to these three, that two had been supplied with an endoprosthesis of the hip on both sides and the third with an irregular surfaced shaft endoprosthesis. In conclusions one can say, despite the problems associated with the modified CT, that it is a suitable additional method for the early diagnosis of the loosening of total hip endoprosthesis.

Aged↗
PubMedDate not supplied

[Self-castration with suicide].

Suicide of a 31 year old man with multiple slashes and stab wounds including complete amputation of penis, scrotum and testicles is reported. Wound patterns in genital self-mutilations are discussed. Despite serious loss of blood the reported survival periods are remarkable; death from bleeding seems to be rare. A review of similar cases shows that self-castrations as well as other self-inflicted genital mutilations are usually associated with psychiatric disorders and transsexuality or hypersexuality.

Adult↗