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Biomedical subjects

W Junge

Publications and source records attributed to W Junge.

At least 127 records · Page 7Linked to original sources

[Possibilities of differenciating mycoplasma serologically and by protein analysis (author's transl)].

The problem of serological differentiation of the various mycoplasma strains and their demarcation from the L-phase variants of bacteria are based on the fact that it is difficult to obtain highly purified antisera which are only directed towards the cell membrane. For this reason an attempt was made by means of diselectrophoresis to determine the plasma and membrane proteins of various mycoplasma strains and L-phase variants and to evaluate them from differential diagnostic point of view. The results suggest that an unequivocal and rational differentiation is possible by this method of examination.

Antibodies, Bacterial↗

Current-voltage studies on the thylakoid membrane in the presence of ionophores.

The reversibility of the binding of ionophores to the thylakoid membrane is studied. While gramicidin binds practically irreversibly, valinomycin and nonactin bind reversibly, however, only a small fraction (about 1%) of the membrane-bound valinomycin or nonactin is active in ion transport. The current-voltage relationship is evaluated under these circumstances. We have found that it is practically linear. This together with the relationship between current and ion concentration agrees qualitatively with the results reported for bimolecular lipid membranes, which contain a large fraction of negatively charged lipids. For the ionophores, valinomycin and nonactin, the binding equilibria (K approximately equal to 10-4) and the turnover numbers (approximately equal to 3-10-4/s) are evaluated for their action on the thylakoid membrane. Possible reasons for the inactivity of the majority of membrane-bound ionophore molecules are discussed.

Anti-Bacterial Agents↗