The rose bengal plate agglutination test in the diagnosis of brucellosis.
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Biomedical subjects
Publications and source records attributed to W J Morgan.
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In 1965, the WHO Expert Committee on Biological Standardization (1966) noted that stocks of the International Standard for Anti-Brucella abortus Serum were almost exhausted and requested the Central Veterinary Laboratory, Weybridge, England, to examine a quantity of serum offered by that laboratory for its suitability to serve as a replacement, and to arrange a collaborative assay. After being distributed into ampoules and freeze-dried, the serum was assayed against the International Standard by 17 laboratories in 12 countries. On the basis of the results obtained, the serum has been established as the Second International Standard for Anti-Brucella abortus Serum and the International Unit has been defined as the activity contained in 0.09552 mg of the Second International Standard.
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It has been shown by a number of workers that Brucella melitensis Rev. 1 vaccine confers good protection against infection with Br. melitensis in sheep and goats. Although Br. abortus strain 19 vaccine is widely used in some countries to protect sheep, it has not been evaluated for caprine brucellosis. The purpose of the experiment reported in this paper was to determine and compare the degree of protection given by living vaccines prepared from Rev. 1 and strain 19 and by a non-agglutinogenic strain of Br. melitensis against graded, experimental challenge in goats.Under the conditions of the experiment, it was clearly shown that the Rev. 1 vaccine gave much better protection than either strain 19 or the non-agglutinogenic strain.There was no relationship between antibody response to vaccination and immunity but there was some correlation between the allergic response and immunity; it is suggested that the allergic response might be used as an initial screening test for evaluating Brucella vaccines.
The Weinstein Enhanced Sensory Test (WEST) is performed using a calibrated monofilament esthesiometer. This study was designed to establish normal values for the WEST when testing digit tip sensibility and to assess whether factors such as age, gender, and side affect normal WEST values. In order to establish normal values, the WEST was performed on 120 subjects who had no clinical evidence of peripheral neuropathy or subjective changes in digit tip sensation. Subjects also completed a questionnaire and underwent neurometric testing of the median nerve and a brief clinical examination. The results of the study indicate that age had a significant effect on WEST values (p = 0.0002) and that there was an interaction effect for age and gender (p = 0.018). There are strong correlations between the WEST values for individual digits for a given subject and between WEST and electroneurometer values. Normal values for the WEST should be interpreted with regard to age and gender. These normal values (defined as the values for 80% of the population of each category) based on the sample used for this study are as follows: for men and women 55 years of age or younger, 0.035 g; for women older than 55 years, 0.15 g; for men older than 55 years, 0.385 g.
A computerized hand and wrist motion analysis system was modified to capture data at a rate of up to 1000 Hz. Using this system, wrist flexion and extension data were collected on five right-handed professional pitchers (75 pitches). A wrist position versus time graph was generated for each pitch. The pitch data produced a reproducible analysis of motion for the majority of the pitches regardless of pitcher. Based on the graphic display of data points, four phases of wrist motion during a pitch were identified. The first phase is the cocking phase, or the motion of the wrist as it moves into maximum extension. This is then followed by the most explosive phase, the acceleration phase, which represents ball propulsion. At ball release, the wrist progresses through flexion and there is a consistent decrease in wrist velocity, known as the deceleration phase. Finally, there is the recovery phase, or the return of the wrist toward neutral. Average values for wrist range of motion, length of phase, and angular velocity (degrees per second) were calculated for each phase of the pitch. This study represents a major step toward quantifying motion of the wrist during a pitch. The ability to quantify this motion may prove valuable in the assessment of throwing athletes after injury and rehabilitation.
Acute hand and wrist injuries in the athlete constitute a unique orthopaedic challenge. Because of the particular demands on the athlete (e.g., financial implications, coaching and administration pressures, self-esteem issues), a specialized management approach is often necessary. Common sites of injury include the ulnar collateral ligament of the thumb metacarpophalangeal joint, proximal interphalangeal joint, metacarpals and phalanges, scaphoid, hamate, and distal radius. Treatment of these injuries varies depending on the patient's age, sport, position played, and level of competition, but departures from standard practice as regards surgery, rehabilitation, and return to competition should never compromise care.
A modified flap design for exposing a palatally impacted canine before orthodontic intervention is described, which utilizes an incision 4-5 mm apical to the free gingival margin. The reflected flap provides good surgical access without violating the integrity of the periodontium.
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