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Biomedical subjects

W J Brzosko

Publications and source records attributed to W J Brzosko.

At least 37 records · Page 2Linked to original sources
PubMed1984

Immunotherapy of chronic active viral hepatitis B with propionibacterium granulosum.

12 HBsAg-positive patients with chronic active hepatitis B were treated monthly with cell walls (5 or 10 mg) of Propionibacterium granulosum KP-45 (PG) intravenously administered for a period of 6-10 months. The clinical state was evaluated according to an arbitrary 6-grade scale based on serum biochemical tests, HBV markers and the morphology of liver biopsy samples. Intravenous administration of PG was well tolerated, and no serious side effects were observed. All patients exhibited complete or partial normalization of biochemical parameters and HB5AG and DNA-polymerase levels were decreasing after 6 months of therapy. Prior to treatment, HBeAG was detected in 9 patients, in 4 of whom seroconversion occurred with appearance of anti-HBeAg. Excellent results were seen in 3 patients and beneficial results in a further 3 patients; signs of improvement were seen in 5 patients, and only one patient did not react in a clearly positive way. In 11 of these patients, before and during treatment, isolated peripheral blood lymphocytes were investigated for their ability to show rosette E formation, their reactivity to phytomitogens (PHA, ConA), and their suppressor T lymphocyte activity. In five patients interferon levels were investigated.

Adjuvants, Immunologic↗
PubMed1979

Detection of measles virus antigen(s) in peripheral lymphocytes from patients with subacute sclerosing panencephalitis.

Peripheral blood lymphocytes from 5 patients with subacute sclerosing panencephalitis (SSPE) were stimulated with phytohemagglutinin (PHA) and analyzed for the presence of the measles virus antigen(s) by immunofluorescence (IF). For detection of viral antigen fluorescein-conjugated globulins from SSPE patients or from measles convalescents both with high anti-measles titer were used. Peripheral blood lymphocytes from 5 children with measles were used as a positive control. Measles virus antigen(s) were localized in PHA-transformed lymphocytes in all SSPE patients. The present results indicate that in SSPE measles-like virus infection may be found not only in the central nervous system, but also in the circulating lymphoid cells.

Adolescent↗
PubMed1977

Immunofluorescence study of brain plaques from two patients with multiple sclerosis.

Immunofluorescence studies were performed on selected plaques from two multiple sclerosis patients. Positive staining revealed the corresponding presence of immunoglobulin G and complement fraction C1q within the plaque areas. These components could be removed only after low pH washing. On the basis of these observations, we discuss the possibility of the presence of immunocomplexes within the multiple sclerosis brain.

Brain↗
PubMed1976

Immunopathologic aspects of Pneumocystis carinii pneumonia in infants as revealed by immunofluorescence and electron microscopy.

Lung tissue, lymph nodes, and spleen from infants 4-15 weeks old who died of Pneumocystis carinii pneumonia were studied by immunofluorescence and immunoelectron microscopy. The results strongly suggest that antibodies to P. carinii synthetized in lungs by inflammatory infiltrates and in regional lymph nodes are essential in the elimination of P. carinii from infected lungs through their opsonization of the P. carinii organisms. Disintegration of P. carinii conglomerates subsequent to the binding of complement preceded their phagocytosis by lung alveolar macrophages. The immunomorphologic findings strongly supported the hypothesis that the replication of P. carinii at the rate leading to clinical symptoms is due to impaired and delayed synthesis both of antibodies to P. carinii and of complement.

Antibody-Producing Cells↗