Search PubMed⌕ Search

Biomedical subjects

W J Baker

Publications and source records attributed to W J Baker.

34 records · Page 2Linked to original sources
PubMed1985

Improved detection of upper abdominal abscesses by combination of 99mTc sulfur colloid and 111In leukocyte scanning.

Indium-111-labeled leukocyte scanning is an excellent technique for detecting abdominal abscesses. However, since labeled leukocytes are normally taken up by the liver and spleen, diagnosing upper abdominal abscesses can be difficult. A prospective study was undertaken to see if having a technetium-99m sulfur colloid liver-spleen scan to compare with the 111In leukocyte scan would improve the reader's ability to diagnose or exclude upper abdominal abscesses. Ninety patients with suspected upper abdominal abscesses were examined with 111In leukocyte scans followed immediately by conventional 99mTc sulfur colloid liver-spleen scans in the same projections. In 57% of patients with upper abdominal abscesses and 26% of all patients studied, the 99mTc sulfur colloid scan was essential to diagnose or exclude an upper abdominal abscess. The liver-spleen scan was considered helpful in another 13%. The addition of a 99mTc sulfur colloid liver-spleen scan to the 111In leukocyte study is useful when evaluating patients with suspected upper abdominal abscesses.

Abdomen↗
PubMed1985

No difference in sensitivity for occult infection between tropolone- and oxine-labeled indium-111 leukocytes.

There is considerable disagreement as to whether oxine or tropolone is the best labeling agent for indium leukocytes. We have previously looked at the sensitivity of oxine-labeled 111In leukocyte scans for occult infections and now present a similar group of patients imaged with tropolone-labeled 111In leukocytes. Thirty-four patients (38 studies) with possible occult infection were prospectively studied. Patients were imaged 1-4 hr after injection and again at 24 hr postinjection. The early tropolone images had a sensitivity of 53% while the delayed images at 24 hr had a sensitivity of 93%. Based on a previous study, oxine-labeled leukocyte scans have an early sensitivity of 33% and a delayed sensitivity (at 24 hr) of 95%. The differences in sensitivity between oxine and tropolone when imaged early and at 24 hr were not statistically significant. We conclude that there is no significant difference in the ability to detect infection between oxine- and tropolone-labeled leukocytes, both early at 1-4 hr, and on delayed imaging 24 hr after injection.

Abscess↗
PubMed1980

A simple method of predicting severe sepsis in burn patients.

Twenty-six patients with major thermal injury were studied with sequential tests of immunocompetence. Five to 8 days after burn, 12 of 26 patients developed a marked depression in the phytohemagglutinin response (17 +/- 8 percent of baseline) and an increase in suppression of the normal mixed leukocyte response (70 +/- 13 percent suppression), which was followed by severe life-threatening sepsis 4 to 5 days later. Concomitant with this marked immunosuppression, the 12 patients developed red debris in the normally white mononuclear layer of the Ficoll-Hypaque density centrifugation gradients used to separate mononuclear cells. None of the 14 patients with minimal or no sepsis developed red debris in Ficoll-Hypaque gradients, nor did they show signs of immune depression by phytohemagglutinin or mixed leukocyte response assays. The only patients in the severe sepsis group who survived were those given aminoglycosides at the time red debris was observed on the Ficoll-Hypaque gradients. The presence of red debris on Ficoll-Hypaque separation appears to be a simple and reliable predictor of impending sepsis, which allows the use of antibiotics before the clinical onset of sepsis.

Adult↗
PubMed1980

A simple device for injection of small calibrated amounts of horseradish peroxidase into the cerebral cortex.

A simple device for injecting horseradish peroxidase into the cerebral cortex is described. It consists of a syringe connected by tubing to a hydraulic adaptor which provides a sealed connection to a disposable glass micropipette. Replaceable micropipettes may be prepared by drawing capillary glass under heat to a fine diameter tip that provides easy penetration into the cortex without dimpling the surface grey matter or damaging the underlying white matter. The hydraulic seal achieved by the adaptor allows volumes of the order of 0.05 microliter to be readily injected into nervous tissue.

Animals↗
PubMed1980

Labeling autologous leukocytes with indium-111 oxine.

A method is described for labeling autologous leukocytes with indium-111 oxine, which is used for localizing inflammatory processes. To an aqueous solution of indium-111 chloride was added sodium acetate buffer, pH 5.0, and then a freshly prepared solution of oxine (8-hydroxyquinoline) in ethanol. After 3-5 minutes of incubation at room temperature, the chelated indium-111 oxine was extracted with chloroform, evaporated to dryness, redissolved in ethanol, and diluted with 0.9% saline. Leukocytes were isolated from venous blood by centrifugation at 300-350 g and resuspended in 0.9% saline. The cells were labeled by adding the indium-111 oxine solution to the leukocyte suspension. Clinical results have shown indium-111-labeled leukocytes to be highly specific for abscess localization. Indium-111 is useful for imaging because its gamma emissions are suitable for external detection and its half-life of 67 hours allows studies to be performed over several days without presenting an excessive radiation dose to the patient.

Abscess↗
PubMed1977

The uphill fight!

Explore the source record for details and available documents.

Dentistry↗