Search PubMed⌕ Search

Biomedical subjects

W Hanna

Publications and source records attributed to W Hanna.

At least 55 records · Page 3Linked to original sources
PubMed1989

Primary multilobulated B-cell lymphoma of the breast.

Malignant lymphomas with multilobulated nuclei are recently recognized neoplasms. We report a case of multilobulated B-cell lymphoma arising in the breast. The light microscopic, electron microscopic, and immunohistochemical features are described and compared with those of previously reported T- and B-cell multilobulated lymphomas from other sites. A follicular center cell origin of this lymphoma is postulated. To our knowledge, this is the first report of a primary multilobulated lymphoma of the breast.

B-Lymphocytes↗
PubMed1988

Clinical trial of low-fat, high-carbohydrate diet in subjects with mammographic dysplasia: report of early outcomes.

Despite evidence that dietary fat intake may influence breast cancer risk, there is little information about the effects of dietary fat on the human breast. We have studied the effects of dietary fat on the breast by examining the influence of dietary fat reduction on mammographic dysplasia (nodular or sheetlike areas of radiological density). Subjects with mammographic dysplasia were randomly allocated to a control group, in which they received advice about maintaining a balanced diet (36% of calories as fat), or an intervention group, in which they were taught to reduce dietary fat to a target of 15% of calories. A total of 295 patients consented to randomization, and after 1 year, 20% of the intervention group and 5% of the control group had dropped out (failed to keep appointments and provide nutrient data). The remaining patients closely adhered to the dietary goals of the study as assessed by food records, chemical analysis of duplicate meals, and serum cholesterol measurements. Comparison of mammograms before and after 1 year of dietary fat reduction shows no significant influence on the extent or density of mammographic dysplasia. Surgical biopsies performed in subjects after entry in the study showed five cancers in the control group and two cancers in the intervention group; this total of seven cancers is four times the number expected. These data show that clinical trials of the effects of dietary fat reduction on breast cancer risk are feasible and that long-term compliance with a low-fat diet can be achieved, and they confirm that the patients selected because they had mammographic dysplasia had increased risk of breast cancer.

Adult↗
PubMed1988

Fine-needle aspiration biopsy of an islet cell tumour simulating pancreatic carcinoma.

The importance of distinguishing between malignant islet cell tumour and pancreatic carcinoma is emphasized in this report of a 57-year-old woman who presented with an epigastric mass. Clinically and radiologically it was diagnosed as a pancreatic adenocarcinoma. A fine-needle aspiration biopsy specimen obtained under ultrasonic guidance showed tumour cells suggestive of an islet cell tumour. Immunostaining and electron microscopy were performed on the aspirate. The tumour cells stained positive with antibodies to keratin, glucagon and gastrin; ultrastructural examination revealed neurosecretory granules, confirming the diagnosis of an islet cell tumour. Angiography was performed to assess the possibility of debulking the mass. This case demonstrates the value of immunohistochemistry and electron microscopy on fine-needle aspiration biopsy specimens of the pancreas to differentiate islet cell tumours, which are potentially curable, from pancreatic adenocarcinomas, which carry a 5-year survival rate of less than 2%.

Adenocarcinoma↗
PubMed1987

Pathologic features of diabetic thick skin.

Skin thickening simulating scleroderma, or progressive systemic sclerosis, has previously been reported in children and adults with insulin-dependent diabetes mellitus. We have studied eighty-nine patients with insulin-dependent diabetes mellitus and twenty-five normal control subjects. Clinical evidence of skin thickening (diabetic thick skin) was found in 22% of patients with insulin-dependent diabetes mellitus and in 4% of control subjects (p less than 0.05). Full-thickness skin biopsy specimens were taken from the forearm of nine patients with insulin-dependent diabetes mellitus and diabetic thick skin, four patients with insulin-dependent diabetes mellitus and clinically normal skin, four patients with progressive systemic sclerosis, and four normal control subjects. The sections stained with hematoxylin and eosin showed increased thickness of the dermis of the forearm skin in all diabetic patients. In diabetic thick skin the collagen bundles were large, disorganized, and separated by clear spaces. Small amounts of acid mucopolysaccharides were present in the upper reticular dermis of five patients with diabetic thick skin. Electron microscopy of the dermis showed capillary basement membrane thickening in Groups 1, 2, and 3. All patients with diabetic thick skin showed active fibroblasts and extensive collagen polymerization in the rough endoplasmic reticulum. Occasional collagen flowers were noted in all patients with diabetic thick skin. Measurements of 100 collagen fibers in the upper and lower reticular dermis of each biopsy specimen showed predominance of large fibers (greater than 60 nm) in Groups 1 and 2. Unlike scleroderma, diabetic thick skin resulted in small fiber sizes (less than 60 nm) only rarely, and bimodality of fiber sizes was not seen.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1987

Cutaneous ultrastructural changes and photosensitivity associated with amiodarone therapy.

Amiodarone, an antiarrhythmic agent, is known to cause photosensitivity and cutaneous hyperpigmentation. Five patients taking this drug for periods of 1 to 48 months were studied. Skin biopsy specimens taken from a sun-exposed site were assessed by light microscopy, electron microscopy, and direct immunofluorescence. Three patients allowed comparative studies to be done on a biopsy specimen from non-sun-exposed skin. Light microscopy findings, including special stains, were not diagnostic of amiodarone-associated cutaneous changes. Electron microscopy, however, displayed distinctive intracytoplasmic inclusions in many cell types, some of which have not been reported previously. These inclusions represent phospholipid membranes associated with amiodarone or its metabolites as the result of a drug-induced lipidosis. Previous reports had postulated the inclusions were lipofuscin. Sun exposure may accelerate the formation of these intracellular deposits because they are more prominent in sun-exposed skin. Four of the above five cases, plus two additional patients, had symptoms compatible with a photosensitivity. Porphyrin assays were normal. Of the six patients phototested, three showed acute reactions to ultraviolet A (UVA) and ultraviolet B (UVB) and significant delayed reactions to UVA and/or UVB. The patients who had normal phototesting were on the drug for shorter periods than those with positive tests.

Adult↗
PubMed1987

Epidermolysis bullosa progressiva.

The first case of epidermolysis bullosa progressiva in the North American literature is described. Electron microscopic examination of a bulla confirmed that blistering is junctional in this rare type of epidermolysis bullosa.

Epidermolysis Bullosa↗
PubMed1987

Phenotypically heterogeneous deletion of the ABH antigen from the transformed bladder urothelium. A scanning electron microscope study.

The deletion of ABH blood group antigens from the luminal surface of the bladder mucosa in cases of well differentiated transitional cell carcinomata, and the formation of pleomorphic microvilli have both been associated with aggressive biological behaviour and invasiveness of the tumors. We have studied cold cup biopsies from 8 normal mucosae and 17 papillary transitional cell carcinomata of the urinary bladder. The aim of our study was to correlate the formation of uniform or pleomorphic microvilli with the extent of deletion of the ABH blood group antigens on the surface of normal and transformed bladder urothelium. Immunogold scanning electron microscopy (SEM) in the backscattered electron (BE) imaging mode was used for this purpose. In the normal urothelium, uniform labeling of the luminal cells was demonstrated. In well differentiated tumors, the superficial cells exhibited uniform microvilli and a heterogeneous expression of the ABH antigens, giving characteristic 'mosaic' patterns of the antigenic labeling across the mucosal surface. These patterns were sharply delimitated at cell junctions when viewed by SEM; these observations were confirmed by transmission electron microscopy. In higher grade tumors, decreased ABH antigen expression, pleomorphic microvilli and/or featureless luminal cells were observed. In the transformed urothelium, the formation of uniform microvilli appeared to precede the loss of ABH antigen in most cases.

ABO Blood-Group System↗
PubMed1986

Signet ring lymphoma of the skin: ultrastructural and immunohistochemical features.

Signet ring lymphoma is a recently described morphologic variant of B cell lymphoma. We are reporting the first case of this subtype of follicular lymphoma that was initially confined to the skin and remained so. Light microscopy showed the tumor to be composed of monomorphous plump epithelioid cells with abundant eosinophilic cytoplasm involving the dermis and subcutaneous fat. Cytoplasmic vacuoles were present within some of the tumor cells, suggesting the diagnosis of adenocarcinoma with a signet ring appearance. Some of the vacuoles were positive on periodic acid-Schiff with diastase staining, and no mucin was identified. Immunostaining showed the presence of intracytoplasmic IgG heavy chains and kappa light chains. Characteristic intracytoplasmic inclusions, which were formed by saccules and microvesicular bodies, were seen by electron microscopy. The diagnosis of signet ring lymphoma was made. No systemic involvement was found. Over a period of 7 years, multiple new skin lesions occurred, all of which responded to radiation therapy. This rare variant of follicular center cell lymphoma, which can occur in the skin, should not be confused with metastatic mucinous adenocarcinoma and malignant melanoma.

Aged↗
PubMed1986

Morphologic and immunohistochemical features of two spontaneous peripheral nerve tumors in Wistar rats.

Morphologic features and S-100 protein immunoreactivity of a benign and malignant peripheral nerve sheath tumor were studied in two Wistar rats. Neoplasms that developed in untreated control rats from tumor bioassays were S-100 protein positive and had similar histopathologic features. Each peripheral nerve sheath tumor was encapsulated and composed of spindle cells arranged around small thin-walled blood vessels. Palisaded tumor cells were in the benign peripheral nerve sheath tumor while cells of the malignant peripheral nerve sheath tumor had cellular atypia and moderate numbers of mitoses. Ultrastructural examination of the malignant peripheral nerve sheath tumor revealed cells with external lamina and interdigitation of cytoplasmic processes. Intracytoplasmic concentric lamellae were seen; they were regularly spaced with a periodicity of about 15 nm. Such structures, indistinguishable from myelin sheaths, have not been commonly associated with peripheral nerve sheath tumors in man. Electron microscopy and immunohistochemistry were useful in the diagnosis of these tumors as Schwannomas and in differentiation from other spindle cell tumors.

Animals↗
PubMed1985

Ultrastructural and immunohistochemical characteristics of mucoepidermoid carcinoma of the breast.

Mucoepidermoid carcinoma is a rare subtype of breast carcinoma. In an attempt to elucidate the histogenesis of these tumors, two mucoepidermoid carcinomas were studied electron microscopically and immunohistochemically with antisera to S-100 protein and epidermal cytokeratin. Light microscopic examination showed these tumors to be composed of mixtures of neoplastic mucus-secreting, squamous, and intermediate cells. Ultrastructural examination revealed squamous cells, mucus-secreting luminal cells, and classic myoepithelial cells. The intermediate cells, which contained focal peripheral myofilaments with dense bodies and pinocytotic vesicles associated with centrally aggregated tonofilaments, were interpreted as modified myoepithelial cells. Classic dendritic myoepithelial and intermediate (modified myoepithelial) cells stained with antisera to S-100 protein and epidermal cytokeratin. Both the ultrastructural and the immunohistochemical features indicate that myoepithelial and duct luminal cells play an important role in the histogenesis of mucoepidermoid tumors of the breast.

Adult↗
PubMed1985

Cicatricial junctional epidermolysis bullosa.

Six types of junctional epidermolysis bullosa (JEB) have been described previously. Each of these types may develop atrophy after recurrent blistering but healing with scar formation does not occur. We describe three cases with a new type of JEB that we have designated as cicatricial junctional epidermolysis bullosa (CJEB). This type is characterized clinically by blisters that heal with scarring and may result in syndactyly and contractures and by stenosis of the anterior nares. These cases were all believed to represent a dystrophic type of epidermolysis bullosa (EB) clinically and emphasize the necessity for electron microscopy of the skin in all EB patients with dystrophic features in order to make a definite diagnosis as to type. Electron microscopy revealed junctional bullae associated with rudimentary hemidesmosomes. Breaks in the basement membrane were noted in addition to active fibroblastic proliferation and laying down of new collagen.

Adult↗
PubMed1985

Hereditary epidermolysis bullosa.

Hereditary epidermolysis bullosa (EB) is a group of disorders of skin characterized by formation of blisters following minor trauma. There are at least sixteen types of hereditary EB. These types are reviewed in this article. There are several classifications of hereditary EB, and they are discussed, as well as current ideas on pathogenesis. Last, the treatment of this often recalcitrant group of diseases is discussed.

Adult↗
PubMed1984

A study of a Caucasian family with variant von Willebrand's disease in association with vascular telangiectasia and haemoglobinopathy.

A family was identified which carries multi-haematological disorders including Type IIA von Willebrand's disease, vascular telangiectasia, and a haemoglobinopathy (haemoglobin S trait). In the affected individuals, the von Willebrand's disease varies in its expression from an asymptomatic form to a severe form especially in those patients with telangiectasia. Some patients have vascular telangiectasia in the mucous membranes of the mouth and lips. In two patients endoscopy disclosed telangiectasia in the mucous membranes of the gastrointestinal tract. All of the patients who had telangiectasia also had von Willebrand's disease. An incidental finding was the presence of an abnormal haemoglobin (haemoglobin S) in some family members. The pattern of inheritance of the haemoglobinopathy was unrelated to the inheritance pattern of von Willebrand's disease. The presence of haemoglobin S did not interfere with the aggregation of platelets in response to ristocetin.

Adult↗
PubMed1984

The value of immunohistochemistry in increasing diagnostic precision of undifferentiated tumours by the surgical pathologist.

The availability of polyclonal and monoclonal antibodies allows immunohistochemical staining (immunofluorescence and immunoperoxidase) procedures to be used by the surgical pathologist, so as to obtain a definite diagnosis in cases where light microscopic examination of tissue sections from biopsy and surgical specimens in inconclusive. Four cases of undifferentiated tumours are described in which only a differential diagnosis could be obtained using light microscopy but a definite diagnosis was achieved when additional information was provided by immunohistochemistry. A scheme is presented for the use of immunohistochemistry to increase diagnostic precision in assessing tumours.

Adolescent↗
PubMed1984

Lichen amyloidosus. Ultrastructure and pathogenesis.

The ultrastructure of six cases of lichen amyloidosus was studied with special attention to epidermal keratinocytes and the role of tonofilaments as precursors of fibrils of amyloid. Through the process of apoptosis, keratinocytes undergo degeneration and become filamentous cells and then filamentous masses or Civatte bodies. These bodies then drop into the dermis through a damaged basement membrane. In the papillary dermis, islands of amyloid become closely associated with Civatte bodies. In some cases, conversion to straight nonbranching filaments, characteristic of fibrils of amyloid, was found within whorled, densely packed filamentous masses. The transformation into fibrils of amyloid was not observed in keratinocytes or Civatte bodies situated in the epidermis. This final step of conversion may be aided by dermal fibroblasts that are frequently lodged around deposits of amyloid.

Adult↗
PubMed1984

Histiocytic sarcoma in Wistar rats. A light microscopic, immunohistochemical, and ultrastructural study.

Histiocytic sarcoma, a recently described tumor entity in rats, was studied by light microscopy in 20 male and female Wistar rats. The tumors originated from subcutaneous tissues; metastasis involved primarily the liver with sinusoidal spread and the lungs with peribronchiolar distribution. The characteristic features of this tumor were the uniform population of tumor cells, palisading necrosis, and abundant multinucleated giant cells. Immunocytochemical and ultrastructural findings confirmed the histiocytic nature of the tumor cells.

Animals↗