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W Gillespie

Publications and source records attributed to W Gillespie.

28 records · Page 2Linked to original sources
PubMed1981

Interaction of alpha-dansylated peptide inhibitors with porcine pepsin: detection of complex formation by fluorescence energy transfer and chromatography and evidence for a two-step binding scheme.

Peptide inhibitors, specifically labeled at the alpha-amino terminus by dansylation, have been prepared by utilizing solid-phase peptide synthesis. Changes in fluorescence have been observed upon mixing these peptides with porcine pepsin that can be attributed to the formation of at least two complexes. Energy transfer between tryptophan residues of the protein and the dansyl group of the inhibitors has been detected by the unique excitation spectra generated. The kinetics of formation of the second complex can be correlated with inhibition of the catalytic activity of pepsin. Evidence for complex formation has also been obtained from gel filtration experiments using the fluorescent peptides.

Animals↗
PubMed1978

Elastofibroma. A pseudotumor of myofibroblasts.

The fine structure of the cell of origin in elastofibroma showed features of both fibroblasts and smooth muscle cells with fibrils that formed parallel stacks 100 A in diameter and 100 A apart and occupied large areas of the cytoplasm of some cells and an abundance of rough endoplasmic reticulum. The myofibroblast has been reported in a variety of lesions and is also present in granulation tissue and hypertrophic scars. It is postulated, in accordance with previous reports, that myofibroblasts are instrumental in the repair process and that elastofibromas are a product of constant subclinical trauma and repair.

Aged↗
PubMedDate not supplied

The public vs private debate: separating facts from values.

The choice between public and private emergency ambulance services is generally based on histological experience within the community. No empirical evidence exists that supports an argument that either public or private emergency ambulance services are better, per se. On a macro level, this debate is based on the question of the role of government and the role of the marketplace in the delivery of public services and medical care, and the comparative efficiencies of public and private organizations. On a micro or community level, these philosophical concerns are supplemented with issues relating to protection of individual jobs and investments, upholding of community tradition, and maintenance of existing relationships. Other specific values that are considered include the role of profit and equity--fairness of coverage. A rational choice would be based on consideration of efficiency and effectiveness. The effectiveness of an emergency medical services system is primarily based on its ability to provide patients with the level of care that they need within a clinically appropriate time. Efficiency is the ratio between inputs and outputs. One factor that can increase efficiency is the availability of excess production capacity that can be used to provide emergency ambulance service, with a low marginal cost of adding this to the other functions. A rational model is intended to change the level of the debate to one that is less based on values, but it is impossible for a community to select an ambulance provider in a value-free environment.

Ambulances↗