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Biomedical subjects

W G John

Publications and source records attributed to W G John.

28 records · Page 2Linked to original sources
PubMed1986

Glycosylated haemoglobin levels in patients referred for oral glucose tolerance tests.

Oral glucose tolerance tests (OGTTs) were performed on 127 patients, and the results interpreted according to the WHO criteria. Glycosylated haemoglobin (GHb), assayed by affinity chromatography, was measured on the fasting blood specimen. All patients classified as diabetic were found to have GHb results greater than 9.9%. There was no overlap with the normal group, all of whom had GHb results less than 8.8%. In the diabetic patients, GHb showed good correlations with both fasting and 2-hour post-glucose blood glucoses. By contrast the non-diabetic group showed only poor correlations between GHb and the fasting and the 2-hour blood glucose concentrations. Overall, the best correlation was found between GHb and the mean of the five blood glucose results obtained during the OGTT.

Blood Glucose↗
PubMed1985

Glycosylated haemoglobin and glycosylated albumin in non-diabetic and diabetic mothers, and their babies.

Glycosylated haemoglobin (GHb), glycosylated albumin (GAlb), and blood glucose were measured from 283 non-diabetic, 7 insulin-dependent diabetic, and 5 gestational diabetic mothers, and in the cord blood of their babies. Significant correlation was found between mother and baby for GHb (r = 0.31) and GAlb (r = 0.42). However, GHb, GAlb and glucose concentration were significantly higher in maternal blood compared to cord blood (p less than 0.001). Only GAlb showed any difference between a pregnant and a non-pregnant population (p less than 0.001). All three parameters were significantly higher in the diabetic mothers compared to the non-diabetic mothers (p less than 0.001). No difference was found between the levels of GHb and GAlb in babies from these two groups. There was no difference in the level of GHb between gestational and insulin-dependent mothers although the latter showed a significantly higher blood glucose (p less than 0.05).

Adult↗
PubMed1985

Affinity chromatography: a precise method for glycosylated albumin estimation.

A method is described for the estimation, in plasma, of glycosylated albumin, using the commercially available affinity chromatographic material Glycogel B (immobilised m-aminophenylboronic acid on an agarose support) to separate the glycosylated and non-glycosylated albumin, followed by the analysis of albumin concentration using rocket immunoelectrophoresis. Glycosylated albumin and glycosylated haemoglobin showed good correlation (r = 0.91) when both are estimated by affinity chromatography. The glycosylated albumin method displays good within-batch (2.8-4.4% CV) and between-batch (2.9-5.4% CV) precision, and the method is not affected by haemolysis. Using this method a reference range of 2.0-5.4% was found for glycosylated albumin. Levels of glycosylated albumin in diabetics (10.06 +/- 3.23%) were found to be significantly higher (P less than 0.001) than those in non-diabetics (3.72 +/- 0.85%). It was found that loading more than 3 mg of albumin on to a 1 mL affinity column must be avoided, as this appears to overload the column.

Anticoagulants↗
PubMed1984

Ulcerative colitis: why is the mortality from cardiovascular disease reduced?

A recent mortality study of patients with ulcerative colitis showed significant deficits in deaths from cardiovascular disease and from respiratory disease in males. The reasons for the observed deficits have been examined by studying the risk factors in a consecutive series of 103 patients with ulcerative colitis using matched controls. The patients and controls were assessed for age, sex, social class, alcohol and tobacco consumption. Height, weight and blood pressure were recorded and fasting blood samples were taken for serum lipid estimation. Patients with ulcerative colitis had significantly lower systolic and diastolic blood pressures and the changes were particularly marked in patients with extensive colitis and after panproctocolectomy. After matching for age, sex and social class the patients with ulcerative colitis smoked significantly less than controls. Serum lipid measurements were similar in both groups. The lower blood pressure may be related to sodium and water depletion particularly in ileostomy patients and those with extensive colitis.

Adult↗
PubMed1983

Proteins of guinea-pig bile: selective resorption in the gall bladder.

We have examined and compared the proteins present in guinea-pig bile as collected either from the common hepatic duct or from the gall bladder. Guinea-pig bile, collected from the common bile duct, has a rather low concentration of protein. Detailed examination shows that the concentrations of actively transported proteins such as immunoglobulin A and haptoglobin:haemoglobin complexes are markedly lower than in rats although the concentrations of proteins which, like albumin, leak non-specifically into bile are similar in the two species. We also find that the protein composition of guinea-pig bile is extensively and selectively modified by resorption of protein in the gall bladder.

Animals↗
PubMed1982

A prospective study of the effect of 12 months treatment on serum lipids and apolipoproteins A-I and B in Type 2 (non-insulin-dependent) diabetes.

Serum high density lipoprotein cholesterol, total cholesterol, triglyceride and apolipoproteins A-I and B were studied at diagnosis and after 12 months conventional treatment in a group of Type 2 (non-insulin-dependent) diabetic patients. No significant deleterious effect on serum lipids and apolipoproteins was seen in any of the subgroups during the treatment period, although in the overall group there was a significant increase in serum total cholesterol in females. Serum high density lipoprotein cholesterol increased significantly in obese males treated with calorie restriction alone. There was a significant increase in serum apolipoprotein A-I in obese females treated with calorie restriction and metformin and in non-obese females treated with carbohydrate restriction and glibenclamide.

Apolipoprotein A-I↗