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Biomedical subjects

W Francisco

Publications and source records attributed to W Francisco.

13 recordsLinked to original sources
PubMed1987

[Stable solution of fuchsin--a new method for preparation of Ziehl-Neelsen staining].

The fuchsin salts currently available in Latin American market have shown some instability when in solution, according to the classic Ziehl-Neelsen method, resulting in a total precipitation of the salt. The authors indicate a new technique for the preparation of this solution, in order to minimize the action of interfering factors responsible for the precipitation, obtaining thus a greater solubility of the salt, as well as the solution stability. The method effectiveness is reinforced by the utilization of a smaller amount of the salt and the attainment of a larger storage period for the solution.

Chemical Precipitation↗
PubMed1985

Rapid, automated identification of novobiocin-resistant, coagulase-negative staphylococci.

A modified automated method that uses the MS-2 system (Abbott Laboratories, Diagnostics Div., Irving, Tex.) to verify the reaction of coagulase-negative staphylococci to novobiocin is described. This technique permits the testing of a great number of specimens in an average time of 99 min and results in a 100% match with the traditional method of culturing.

Bacteriological Techniques↗
PubMed1980

Cefoxitin penetration into cerebrospinal fluid in patients with purulent meningitis.

The penetration of cefoxitin into cerebrospinal fluid (CSF) was studied in 25 patients with purulent meningitis treated with antibiotics other than cefoxitin. Each patient received three 2-g doses of cefoxitin at 6-h intervals. Blood and CSF samples were obtained before and at 2, 4, or 6 h after the first and third doses. CSF cefoxitin concentrations were found in all patients and varied between 1.2 and 22.0 microgram/ml, with a majority of the concentrations falling within a range from 1.2 to 6.2 microgram/ml. The concentrations tended to be higher in CSF samples drawn after the third cefoxitin dose than in those drawn after the first cefoxitin dose, indicating an accumulation of cefoxitin in CSF with repeated doses. Peak cefoxitin concentrations in CSF seemed to occur between 2 and 6 h after intravenous administration of the drug since the highest concentrations were found in patients from whom CSF samples were taken 4 h after the doses. In patients with bacterial meningitis, it should be possible to achieve therapeutic cefoxitin levels in CSF by using nontoxic doses of the antibiotic.

Adult↗
PubMedDate not supplied

Minute treatment with thiamphenicol in water for acute gonococcal urethritis in male patients.

Eighty-two male patients with acute gonococcal urethritis were given a single oral dose of 2.5 g of granulated thiamphenicol dissolved in water, and the results of treatment were evaluated after 48-72 hr and one week. Of the 76 patients who returned for the first follow-up examination, 75 (98.7%) no longer had Neisseria gonorrhoeae in urethral smears or cultures. Five of these patients did not return for the second follow-up examination, and another four who did return were found to be reinfected as a result of reexposure. Of the remaining 67 patients, 66 (98.5%) no longer had N. gonorrhoeae in urethral smears or cultures. Thus, the overall success rate among the 71 patients who completed the study (with the four cases of reinfection considered instances of failure of therapy) was 93%. The ease of administration, absence of adverse reactions, low failure rate, and low incidence of residual urethral secretions (4.5%) justify the use of thiamphenicol as the drug of choice for the treatment of acute gonococcal urethritis in male patients.

Acute Disease↗
PubMedDate not supplied

Serum penicillin concentrations after intramuscular administration of benzathine penicillin G in children with rheumatic fever and controls.

Serum and urine penicillin levels were determined in 11 children with rheumatic fever (RF) who were receiving benzathine penicillin G (BPG) prophylactically every 3 weeks and in 10 children without RF who received the drug for the treatment of other infections. The dose given was 600,000 units for children weighing less than 25 kg and 1,200,000 units for those with a weight above 25 kg. Blood and urine samples were collected from both groups before and on days 7, 14 and 21 after BPG administration. Our results showed that: minimum inhibitory concentrations (MICs) of BPG for group A beta-hemolytic streptococci were 0.02 IU/ml or 0.0125 microgram/ml; intramuscular BPG did not give adequate serum levels to block the growth of group A beta-hemolytic streptococci in approximately 24 and 62% of children included in the study on days 14 and 21 after its administration, respectively; BPG metabolism was similar in both groups and did not depend on the underlying disease; serum and urine levels did not vary according to sex and weight; and there was a small correlation between serum and urine levels.

Adolescent↗