[Determination of the frequency of Y-chromatin positive spermatozoa in relation to the parameters of spermiograms].
The percentage of y chromatin positive spermatozoa had been determined using the fluorescence microsopce technique. After staining procedure with Atebrin we counted 44.1 (+/- 3.9)% y poistive sperms, after staining with Quinacrine mustard the amount was 48,4 (+/- 4,3)%. With statistical methods we examined the correlation of the percentage y sperms to the single parameters of the semen analysis. So the sperm count, the motility, morphology, pH and fructose of seminal plasma had been compared with their influence upon the X-Y relationship of spermatozoa. In conclusion of our results there was no considerable variability in the X-Y sperm ratio by the different parameters of the semen analysis.
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