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W F GOEBEL

Publications and source records attributed to W F GOEBEL.

At least 19 recordsLinked to original sources
PubMed1963

Colanic acid.

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Escherichia coli↗
PubMed1962

Colicine K. V. The somatic antigen of a non-colicinogenic variant of E. coli K235.

The somatic antigen of the non-colicinogenic bacillus E. coli K235 L(-)OC(-) has been isolated, and its chemical and serological properties have been compared with those of colicine K. The antigen of the non-colicinogenic bacillus has a protein content significantly lower than that of the C(+) antigen, a difference which might be related to the antibacterial activity of the latter. The lipocarbohydrate components of the two antigens are chemically very similar; both contain the same proportions of galactose, glucose, heptose, rhamnose, glucosamine, and mannosamine. When tested by agar diffusion, the two antigens are indistinguishable, as are their lipocarbohydrate components. Our studies indicate that the bactericidal activity of colicine K does not reside in its lipocarbohydrate but in its protein component.

Anti-Bacterial Agents↗
PubMed1962

The isolation of colicine V and a study of its immunological properties.

Colicine V has been obtained from the culture medium in which the colicinogenic bacillus E. coli K357 L_T is grown. The material is electrophoretically homogeneous and proves to be a lipocarbohydrate protein complex identical with the type-specific O antigen of the parent bacillus. Colicine V is toxic both for mice and for rabbits and readily stimulates the elaboration of precipitins and bacterial agglutinins, as well as antibodies which neutralize the antibacterial activity of the colicine itself. The colicine is also toxic for certain strains of Enterobacteriaceae. Although colicine V and colicine K, previously described in this laboratory, have many properties in common, they exhibit no cross-serological relationship whatsoever.

Animals↗
PubMed1961

Colicine V.

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Anti-Bacterial Agents↗
PubMed1960

Colicine K. IV. The effect of metabolites upon colicine synthesis.

The synthesis of colicine K by the colicinogenic bacillus E. coli K(235) has been studied in the chemostat. It has been found that colicine production is influenced by the pH of the medium, by the nature of the primary carbon source, and by the generation time of the microorganism.

Anti-Bacterial Agents↗
PubMed1958

Colicine K. III. The immunological properties of a substance having colicine K activity.

By immunological means it has been shown that colicine K is associated with the O antigen of the colicinogenic bacillus E. coli K(235) L(+)OC(+). The colicine K-O antigen complex elicits the formation of at least two types of antibodies, one a precipitin, the other a colicine-neutralizing antibody. The first precipitates colicine K without neutralizing it, the second neutralizes the colicine without precipitating it. Unlike the purified colicine K complex, the colicine protein component of the O antigen is precipitable by the neutralizing antibody. There is no demonstrable serological relationship between colicine K and phage T(6). These two agents must be considered to be separate and distinct entities.

Anti-Bacterial Agents↗
PubMed1956

Colicine K. I. The production of colicine K in media maintained at constant pH.

An apparatus for maintaining the pH of an actively growing bacterial culture at constant level has been described. Using this apparatus it has been shown that the production of maximal amounts of colicine K from a strain of E. coli known as K235 is dependent upon an enriched nutrient medium which is maintained at pH 7.0.

Anti-Bacterial Agents↗
PubMed1955

Lysis of T4 phage by the specific lipocarbohydrate of phase II Shigella sonnei.

When the specific lipocarbohydrate of Phase II Sh. sonnei and T(4) phage react in vitro, the virus is rapidly inactivated and the content of the viral membrane is released into the surrounding medium. The reaction between phage and lipocarbohydrate proceeds only in the presence of a lipide constituent which can be extracted from the polysaccharide, rendering the latter inactive, and which can be replaced by certain fatty acids. It has been suggested that the lipocarbohydrate is the receptor substance of the Phase II bacillus which specifically combines with and brings about disintegration of the virus when the latter infects the host cell.

Bacteriophage T4↗
PubMed1955

Colicine K.

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Anti-Bacterial Agents↗
PubMed1954

The nature of prophage in lysogenic Bacillus megatherium.

1. By complement-fixation tests it has been shown that the lysogenic and sensitive strains of B. megatherium share one or more antigenic components which are serologically related but not identical. 2. Bacilli of the lysogenic strain of B. megatherium, when grown under conditions such that little extracellular phage is produced, fail to evoke antibodies in rabbits which react either in complement-fixation or neutralization tests with purified megatherium T phage. From this it must be concluded either that prophage is not antigenic or that any antibody which it might elicit does not react with the antigens of the mature virus. The observations reported in this communication accord with the hypothesis that prophage is a genetic structure.

Bacillus↗