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Biomedical subjects

W Erler

Publications and source records attributed to W Erler.

At least 55 records · Page 3Linked to original sources
PubMed1979

[Deoxyribonucleic acids from Pasteurella multocida and Pasteurella haemolytica].

Pasteurella multocida and P. haemolytica strains contain between 1.5 and three per cent phosphorus, between nine and 14 per cent nitrogen, between two and four per cent DNA, and between five and 18 per cent RNA, the precise figures depending on culturing conditions. High-molecular DNA may be isolated by means of bacteriolysis, using deoxycholate or dodecylsulphate and the usual steps of purification, with yield and purity differing by strains. DNA with sufficient purity can be obtained from Sepharose 2 B by gel chromatography. The isolated DNA yields were characterised, base values being between 37 and 38 per cent GC for P. haemolytica and between 41 and 48 per cent GC for P. multocida. Highly suitable precursors to DNA synthesis for tritium labelling are 3H-thymidine, which is incorporated in excess of 3H-thymine by a factor of 255, as well as 3H-uracil, with its activity being recovered also from the pyrimidine bases of DNA via pyrimidine biosynthesis.

Chromatography, Gel↗
PubMed1977

[Chemically defined and partially defined media for pasteurella multocida and pateurella hemolytica].

Two culturing recipes were suggested for Pasteurella multocida and Pasteurella haemolytica, one partialy defined nutritive defined nutritive medium with 0.5 per cent yeast extract and one chemically defined medium. Both had been composed on the basis of studies into nutrient demand and into the effects of complex nutritive substrates. Both nutritive media are applicable also to culturing other bacterial species. (Among the strains tested so far were streptococci, staphylococci, Escherichia coli, pseudomonas, protest, Salmonella,, Shigella, and Haemophilus.)

Bacteriological Techniques↗
PubMed1977

[The reaction of calves to parenteral administration of cyclopheosphamide].

One single injection of 50 mg/kg live weight cyclophosphamide or more to calves was followed by a latency of few days after which pneumonia or enteritis developed and caused death within one or two weeks. Cyclophosphamide application led to pronounced changes in the white blood count characterised, at the beginning, by rise of neutrophilic granulocytes and decline of lymphocates. Primary rise of granulocytes then was followed by almost complete disappearance of them. In those calves that survived the parameters of the white blood count were not restoredto normal until several weeks had elapsed. While an injection of 30 mg/kgcyclophosphamide usually was tolerated without any visible clinical reaction, it was also accompanied by the above pronounced changes in the white blood count. The activity of the reticulohistiocytary system, as recordable by means of ink and bacterial clearance, was not markedly affected by one single injection of 30mg/kg or 40 mg/kg cyclophosphamide. Calves with cyclophosphamide treatment exhibited unambigous humoral immune response, yet somewhat delayed or reduced in comparison to controls. While one single intravenous injectionof 30 mg/kg cyclophosphamide alone failed to trigger any clinical disease, it proved to be capable of rendering calves more susceptible to pneumonia pathogens. It, therefore, might be suitable for supporting experimental infection and thus facilitating the study of enzootic pneumonia of calf which usually is difficult to reproduce.

Animals↗
PubMed1977

[Studies on calf salmonellosis. 3. Preclinical evaluation of a Smd. Salmonella dublin live vaccine].

Results of preclinical assay, preparation and conservation of a S.-dublin live vaccine based on streptomycin dependent mutants and for oral application are demonstrated and discussed in detail. The used oral vaccine is very good tolerated by calves and results in stable immunity after administration of a daily dose of 5 x 10(10) bis 1 x 10(11) living Smd.-mutants for 10 consecutive days. Complete immunity developes in calves within a period of two weeks after the last antigen administration and persists up to the age of 5 to 6 months. Conserving the vaccine at low temperatures (-15 degrees C) the number of living organism is far-reaching preserved in the first 5 months after preparation. After thawing the vaccine is to be used within a period of 3 days. With respect to the preparation of the live vaccine on semisynthetic nutritiv media some informations are given concerning the improvement of bacterial yield.

Administration, Oral↗
PubMed1977

[Binding relationships of fatty acids in lipid A of lipopolysaccharides from Pasteurella multocida].

Each glucosamine disaccharide of lipid A from Pasteurella multocida contains 2 mols myristic acid and 4 mols hydroxymyristic acid. 3-hydroxymyristic acid was identified as fatty acid with amide linkage. Every 2 mols of glucosamine have linked to them 1 mol myristic acid, 3-hydroxymyristic acid, and 3-myristoxymyristic acid. The results so far obtained reflected the structure of lipid A of enterobacteriaceae.

Binding Sites↗
PubMed1976

[Nutrient demand of erysipelothrix bacteria].

Studies into the nutrient demand of Erysipelothrix insidiosa led to the preparation of a nutritive medium with high bacterial yield which is recommended for quantity breeding of bacteria. The medium was complex and did not contain serum but the following components: peptone S, yeast extract, glucose, Na2HPO4, arginine, and Tween 80.

Animals↗
PubMed1976

[Culture media for Salmonella dublin on a chemically defined basis].

The nutrient demand of S-dublin strains was investigated. Media on chemically defined basis for culturing of S. dublin are recommended with reference to the above studies. The definition includes Na2HPO4, KH2PO4, NaCl, glucose, the amino acids L-asparaginic acid, L-cystine, L-glutamic acid, serine, and L-threonine, and nicotinamide as vitamin component. Such media will be quite favourable for certain culturing problems, but complex substrate (yeast extract, peptone, tryptone) should be added for high yields.

Culture Media↗