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Biomedical subjects

W E Barber

Publications and source records attributed to W E Barber.

6 recordsLinked to original sources
PubMed1991

Romazarit: a potential disease-modifying antirheumatic drug.

The synthesis of a series of substituted heterocyclic alkoxypropionic acids is described. They were evaluated for antiinflammatory effects in two animal models of chronic inflammation; adjuvant arthritis and type II collagen arthritis in the rat. The desired profile of biological activity was characterized by the reduction of inflammation with the coincident restoration toward normal levels of the biochemical markers (acute phase proteins) associated with the inflammatory response, an effect that was not shared by classical nonsteroidal antiinflammatory agents. Romazarit, (Ro 31-3948, 7), 2-[[2-(4-chlorophenyl)-4-methyl-5-oxazolyl]methoxy]-2-methylpropio nic acid, was selected for further evaluation. In contrast to NSAIDs, romazarit was inactive in animal models of acute inflammation, and furthermore it did not inhibit the cyclooxygenase enzyme in vitro or in vivo. Inhibition of interleukin-1-mediated events in vitro has been observed.

Animals↗
PubMed1988

Cyclohexane triones, novel membrane-active antibacterial agents.

The cyclohexane triones are a novel group of synthetic antibacterial agents that are active against gram-positive bacteria, Haemophilus influenzae, and Mycobacterium smegmatis. In general, these compounds behaved in a manner similar to that of hexachlorophene, inhibiting the transport of low-molecular-weight hydrophilic substances into bacteria. Unlike cationic detergents, such as chlorhexidine, they did not cause disruption of the bacterial cytoplasmic membrane over a short time period. The most potent antibacterial cyclohexane trione studied had a reduced ability to inhibit solute transport in comparison with certain less active analogs. Cyclohexane triones may express more than a single type of antibacterial effect.

Anti-Bacterial Agents↗
PubMed1984

UV visualization of inorganic anions by reversed-phase ion-interaction chromatography: factors that control sensitivity and detection.

This paper describes a chromatographic technique and detection scheme for inorganic anion analysis. Factors that affect sensitivity and detection are discussed, including the concentration and molar absorptivity of the ion-interaction reagent (IIR) as well as the retention of the eluite ion relative to the retention of the system peak. An ideal system will employ a low IIR concentration, so that a detection wavelength corresponding to a high IIR molar absorptivity can be used to monitor the eluites. In addition, eluites that are eluted before the system peak have much lower response factors than those eluted after the system peak. Furthermore, eluites that are not well separated from the system peak have response factors that are many times larger than eluites that are eluted either before or after the system peak. Computer simulations were performed that predict these response factors.

Anions↗
PubMed1984

Ultra-violet visualization of inorganic anions by reversed-phase ion-interaction chromatography; factors that control retention and selectivity.

This paper describes the development and characterization of a separation and detection system for the analysis of mixtures of UV-transparent inorganic anions. Retention and separation occurs when a hydrophobic, positively charged paired-ion chromatography (PIC) reagent or an ion-interaction reagent (IIR) is added to the mobile phase of a reversed-phase system. Detection of UV-transparent ions results from a perturbation of the distribution equilibria of the UV-absorbing IIR upon injection of the sample ions. The effect of factors such as the concentration and nature of the buffer, co-ions and IIR as well as an organic modifier are described. The major advantages of this method are that the system is nearly completely nonspecific, the separation system takes advantage of highly efficient reversed-phase columns, rapid separations of 4-6 anions in approximately 6-7 min and good sensitivity with detection limits of less than 1 nmole injected. In addition, no special equipment is required to perform ion analysis by this technique. Only conventional high-performance liquid chromatography pumps, detectors and reversed-phase columns are required.

Anions↗