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Biomedical subjects

W D Wiezorek

Publications and source records attributed to W D Wiezorek.

17 recordsLinked to original sources
PubMed1989

[The fermentation diagnosis and histologic studies in blood and the liver of surviving rats after 1 and 2 administrations of a median toxic dose of parathion methyl. 1. Results of studies on the activities of the plasma enzymes AlAT, AsAT, AP, and gamma-GT].

Clearly pronounced changes in plasma enzyme activities were observed 24 hours after one and two applications of parathion methyl (PM). The most strongly pronounced rise was in activity of aspartate-amino transferase and was recorded from both sexes. PM application caused sizeable changes in male rats. Comparison between reactions after one and two applications revealed more intensive changes in males after the 1st application. Somewhat larger differences were recordable in some cases between the 1st and 2nd applications. Closer agreement between reaction intensities after the 1st and 2nd PM applications was recordable from female rats. In either sex in response to one single and repeated application parameters returned to normal within nearly one and the same period of time. No secured relationships between activities prior to and after application of PM could be derived from the assays of gamma-glutamyl transpeptidase (gamma-GT). Both increase and decrease of determined enzyme activities were actually recorded. Activities were highly variable in the control groups. Extreme rises were recordable in some cases from both control and experimental groups. No conclusions so far can be drawn as to "normal values" in rat plasma, possible sex-related differences, and behaviour of enzyme activity in response to one single or repetitive applications. Hence, within common investigations of acute toxicity of alkylphosphates, gamma-GT was found to be not suitable for detection of damage to the liver or other organs.

Alanine Transaminase↗
PubMed1989

[The fermentation diagnosis and histologic studies in blood and the liver of surviving rats after 1 and 2 administrations of a median toxic dose of parathion methyl. 2. Tyrosine aminotransferase and pathologico-morphological findings in the liver].

Tyrosine-amino transferase, an enzyme induced to the liver via glucocorticoids, caused clearly measurable activity rises in either sex, 24 hours after 1 and 2 applications. Increased levels continued to recordable from males on the 3rd day from application, whereas enzyme activities in females had already returned to normal by that time. Histological examination of liver sections, following 1 and 2 applications of parathion methyl (PM), revealed the tissue pattern of clearly manifest toxic hepatosis with detectable mesenchymal reactions. Histologically detectable damage reached its maximum on the 2nd and 3rd days from application. Sex-related differences were not recordable. Damage in response to the 2nd application was characterised by faster mesenchymal activation and higher frequency of cell necroses. The morphological picture of the liver was rated as having returned to normal, 14 days after 1 and 2 applications of PM. These results should not be interpreted as evidence to the involvement of specific, non-cholinergic mechanisms in the processes of damage observed. The alterations might rather be attributed to unspecific disorders in the energy balance or to the effect of "stress" during intoxication.

Animals↗
PubMed1975

[Effects of diethyldithiocarbamate on the acute toxicity and the acetylcholinesterase inhibition by methylparathion in mice].

Acute toxicity of methylparathion male mice is reduced by DET in dose-range from 50 to 300 mg/kg. Mean survival time in LD100 range of methylparathion is significantly prolonged. Inhibition of AChE in vivo is reduced, too. The observed dose-effect-relations show an approx. 10-fold increase in equi-effective doses after DET-pretreatment. DET has no protective effect both in vivo and in vitro, if methylparathion is added to the brain homogenate of mice. As probable mechanism of action a change in methylparathion-metabolism induced by DET in the system of mixed functional oxidases is discussed.

Administration, Oral↗