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Biomedical subjects

W D Seybold

Publications and source records attributed to W D Seybold.

7 recordsLinked to original sources
PubMed1980

Human amniotic fluid contains fibroblast-pneumonocyte factor.

We have studied 41 samples of human amniotic fluid collected at 27-40 wk gestation. After 'stripping' with Florisil, most fluids studied stimulated [3H] choline incorporation into saturated phosphatidylcholine by human fetal alveolar Type II cells. The activity declined with advancing gestation and was inversely related to cell number following incubation. Following acid-ethanol extraction, activity eluted from Sephadex G75 columns in 1 M acetic acid with a Kd of approximately equal to 0.4-0.6, and was greater at earlier gestations (27-31 wk) than at later periods (32-36 or 37-40 wk). In contrast, chromatography at neutral pH without prior extraction resulted in retarded elution. The active material was shown to be heat-stable. Thus, the biochemical features of activity recovered from human amniotic fluid are identical to fibroblast-pneumonocyte factor recovered from supernatant culture media of cortisol-treated human fetal lung fibroblasts.

Amniotic Fluid↗
PubMed1976

Diffuse malignant mesothelioma.

Thirteen cases of diffuse malignant mesothelioma, 12 pleural and one peritoneal, were examined and reviewed from the clinical and morphologic points of view. The most frequent symptom and sign were pain and effusion. Periods of survival varied, with one patient surviving 11 years despite morphologic features of the pleural tumor. The diagnosis can be made only on the basis of excised tumor tissue. The best control of the tumor is attained by repeated surgical removal. Neither radiation nor chemotherapy altered significantly the growth of the tumors.

Adult↗
PubMed1975

Phenol oxidase activation in Drosophila: a cascase of reactions.

Our earlier evidence concerning the complexity of the activation process for Drosophila phenol oxidase has been confirmed by separation and purification of six proteins involved. This is a minimal number required in a reaction series or cascade to yield active enzyme, and at least two more proteins are known to be involved. A simpler system involving only the last step with one precursor and one activation as has been reported in the literature is consistent with the cascade picture, but the whole complex system must be considered when dealing with genetic and developmental regulation of pigmentation and sclerotization. Details are given for partial or complete purification of six of the proteins involved and evidence for their modes of interaction is presented.

Animals↗