Nuclear magnetic resonance determination of thymine nearest neighbor base frequency ratios in deoxyribonucleic acid.
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Animals↗
Biomedical subjects
W D Phillips
Publications and source records attributed to W D Phillips.
High-resolution nuclear magnetic resonance spectroscopy. Advances in instrumentation in this field are leading to new applications in chemistry and biology.
The rapid development of NMR spectroscopy has been characterized by a succession of discrete, significant advances in instrumentation, as well as by less dramatic but cumulatively important improvements in instrument performance, experimental techniques, spectral analysis, and theory. Most significant are the advances in magnet technology, which within 13 years increased the available field strengths from 7.04 to 51.7 kilogauss (with corresponding increase in the PMR frequency from 30 to 220 megahertz). Great improvements in spectrometer stability and in the coupling of spectrometers with on-line computers have so improved sensitivity that some nuclei possessing less favorable NMR characteristics can now be studied.
Alkenes↗
An optical tweezers-based immunosensor for detection of femtomoles-per-liter concentrations of antigens.
We used optical tweezers--optical trapping with focused laser beams--to pull microspheres coated with antigens off of an antibody-coated surface. Using this technique, we could quantify the force required to separate antigen to antibody bonds. At very low surface density of antigen, we were able to detect the single antigen to antibody binding. The force required to break the antigen-antibody bonds and pull the microsphere off the surface was shown to increase monotonically with increasing surface density of antigens. Using the force determination as a transducer, we were able to detect concentrations of free antigens in solution as small as 10(-15) mol/L in a competitive binding assay.
Antigen-Antibody Reactions↗