Search PubMed⌕ Search

Biomedical subjects

W D Morgan

Publications and source records attributed to W D Morgan.

At least 37 records · Page 2Linked to original sources
PubMed1993

An enhanced sensitivity K-shell x-ray fluorescence technique for tibial lead determination.

A novel irradiation-detection geometry capable of enhancing sensitivity for the measurement of tibial lead content by K-shell x-ray fluorescence (XRF) is described. The high-count-rate system comprised a small-area high-specific-activity (0.147 GBq mm-2) 109Cd source and a large-area (nominally 20 cm2) uncollimated detector, forming an axially symmetric back-scattering arrangement. Precisions in the range +/- 4.9 to +/- 14.2 micrograms Pb (g bone mineral)-1 have been obtained in a study of a cohort of 63 controls and 73 workers industrially exposed to lead. These precisions are comparable with those obtained in results using earlier systems, but at reduced source activities (less than 50% of the activity of other systems) and with significant reduction in measurement time (some 30% less than the measurement times of other systems). Subsequent investigation of detector collimation resulted in a marginal improvement in energy resolution, but the restriction in detected photon fluence meant that there was an insignificant change in detection sensitivity. For the resistive feedback preamplifier used in this study a maximum energy rate of the order of 7000 MeV s-1 was found to limit measurement precisions significantly. Higher-count-rate detector systems offer a basis for obtaining mean precisions down to +/- 3 micrograms Pb (g bone mineral)-1 at one standard deviation.

Humans↗
PubMed1993

The measurement of silicon in a lung phantom--a comparison of two nuclear reactions for in vivo activation analysis.

The amount of silica in the human lung may be estimated by measurement of silicon using in vivo neutron activation analysis. A pulsed, fast neutron beam, produced with a 2 MV Van de Graaff generator using the 2H + 2H reaction, was used to irradiate a Si-doped chest phantom in order to determine minimum detection limits (MDL). Two 'in-beam' nuclear reactions on Si were studied; prompt fast neutron inelastic scatter 28Si (n,n' gamma)28Si reaction was measured during the beam burst and the slow neutron prompt capture reaction was measured between the fast neutron bursts. Although the latter reaction appeared less favourable due to neutron cross section and measurement efficiency considerations, it yielded an MDL of 1.8 g compared with 2.3 g for the 28Si(n,n' gamma)28Si reaction. A comparison was made with a 252Cf neutron irradiation system where a Si MDL of 6.3 g was obtained using the slow neutron capture reaction. The Van de Graaff system permits 'exposed' Si lung burdens to be measured but not normal levels. Improved measurement sensitivity may be achieved by reduction of high counting-rate losses and high background radiation.

Humans↗
PubMed1993

The development of a technique to measure bone aluminium content using neutron activation analysis.

The accumulation and toxicity of aluminium in patients with chronic renal failure is a well recognized hazard, and there is a need for a non-invasive technique to assess Al tissue load in these patients. The technique of in vivo neutron activation analysis, using a thermal neutron beam from a reactor, has been employed by previous workers, who measured Al in the hand with a detection limit of 0.4 mg for a dose equivalent of 20 mSv. However, the application of this technique is restricted by the very limited availability of nuclear reactors. We report the modification of an existing 252Cf-based instrument and construction of a shielded, high-efficiency counting system for the in vivo measurement of Al in the hand. Phantoms containing tissue-equivalent solutions of Ca, P, Na and Cl with various Al loadings were used for validation of the technique. The Al/Ca ratio in the hands of seven patients with renal failure was measured using a cyclic activation technique to compensate for the relatively low neutron output of the 252Cf source, and a detection limit of approximately 2.2 mg Al was achieved for a dose equivalent of 36 mSv. The results were compared with the Al content of iliac crest bone biopsy specimens measured using electrothermal atomic absorption spectrophotometry.

Adult↗
PubMed1993

A five-compartment model of body composition of healthy subjects assessed using in vivo neutron activation analysis.

A body composition study of 31 healthy subjects covering a wide range of age (23.5-72.0 years) and weight (44.5-104.2 kg) has been undertaken. Subjects were assessed by in vivo neutron activation and tritiated water analysis and values of total body nitrogen, hydrogen and fat obtained by utilization of a five-compartment model of body composition comprising protein, water, fat, minerals and glycogen. The protein (as 6.25 x nitrogen) and water compartments were measured but the smaller compartments of minerals and glycogen were calculated as fixed fractions of the fat-free mass estimated from the water space. Fat was calculated as the body mass less the sum of the four other compartments. Mean values (+/- SEM), expressed as a percentage of body mass, for nitrogen, hydrogen and fat were 2.56 (+/- 0.07)%, 10.07 (+/- 0.04)%, and 21.9 (+/- 1.7)% respectively for men and 2.14 (+/- 0.07)%, 10.40 (+/- 0.04)%, and 35.5 (+/- 1.7)% respectively for women. The accuracy of the nitrogen measurements was evaluated by comparison with calculated values from two prediction equations; correlation coefficients, the mean bias (estimated from the mean differences between the measured and predicted nitrogen), the confidence interval for the bias, and limits of agreement were calculated. The correlation coefficients were high (r > 0.93) and the mean bias indicative of agreement. The ratio of nitrogen to the fat-free mass (derived from the body composition model) was also calculated and mean values (+/- SEM) of 32.7 (+/- 0.4) and 33.1 (+/- 0.4) g/kg for men and women, respectively were obtained. The hydration of the fat-free mass was determined to be 0.725 (+/- 0.002) and 0.722 (+/- 0.002) kg/kg for men and women respectively. The accuracy of the body fat estimate was evaluated by comparison with skinfold-thickness-derived values and computation from tritiated water space. The ratio of the body composition model to skinfold-thickness-derived fat was significantly (P < 0.005) greater than unity. The mean bias between the body composition model and tritiated-water-derived fat was -0.6 percentage points of fat (95% confidence interval from -0.3 to -0.9 percentage points of fat). Finally a prediction equation (r2 = 0.908, SEE = 108 g) for body nitrogen in healthy subjects based on weight, age and sex was calculated.

Adipose Tissue↗
PubMed1992

Changes in total body nitrogen during weight reduction by very-low-calorie diets.

Body composition measurements, including total body nitrogen (TBN) by in vivo neutron activation analysis, were made on 11 female volunteers before and after an 11-wk very-low-calorie diet (VLCD) [1695 kJ (405 kcal) 6.7 g N]. Mean body mass index (BMI) changed from 32.1 to 26.2 kg/m2, corresponding to a mean weight loss of 16.2 +/- 2.4 (SD) kg. The mean loss of TBN was 125 +/- 57 g, equivalent to 781 +/- 356 g protein. The fat-free mass (FFM) component of the weight loss was calculated by two different methods as 23.5% (+/- 3% SEM) and 22.8% (+/- 2.7% SEM), respectively, thereby demonstrating the improved protein sparing of ketogenic VLCDs. FFM loss was not clearly related to BMI.

Adult↗
PubMed1991

Total body calcium in patients with inflammatory bowel disease: a longitudinal study.

1. Serial measurements of total body calcium have been made by prompt gamma-neutron activation analysis in 13 patients with inflammatory bowel disease over a mean period of 23 months. Changes in spinal trabecular bone mineral density and radial shaft bone mineral content were also assessed by using quantitative computed tomography and single photon absorptiometry, respectively. 2. The mean annual decreases (95% confidence intervals) were: total body calcium, 7.8% (-12.0 to -3.7%; P less than 0.001); spinal trabecular bone mineral density, 2.5% (-5.0 to +0.1%; 0.05 less than P less than 0.1), radial bone mineral content, 2.1% (-3.4 to -0.8%; P less than 0.01). 3. No significant correlations were found between rates of change of the three variables. However, there were significant positive correlations between the baseline values for total body calcium and radial bone mineral content (r = 0.638, P less than 0.05), spinal bone mineral density and radial bone mineral content (r = 0.854, P less than 0.01), and total body calcium and spinal bone mineral density (r = 0.876, P less than 0.001). 4. These results demonstrate rapid decreases in total body calcium in patients with inflammatory bowel disease which, in conjunction with the significant decrease in radial shaft bone mineral content, indicate increased rates of cortical bone loss. Whilst values for bone mass at different skeletal sites showed positive correlations within individuals, no relationship was found between the rates of change in bone mass at these sites. 5. The rapid bone loss observed in some subjects emphasizes the importance of early detection of osteoporosis by bone densitometry and the need for effective prophylactic measures to be established in this group of patients.

Absorptiometry, Photon↗
PubMed1991

Modular recognition of 5-base-pair DNA sequence motifs by human heat shock transcription factor.

We investigated the recognition of the conserved 5-bp repeated motif NGAAN, which occurs in heat shock gene promoters of Drosophila melanogaster and other eukaryotic organisms, by human heat shock transcription factor (HSF). Extended heat shock element mutants of the human HSP70 gene promoter, containing additional NGAAN blocks flanking the original element, showed significantly higher affinity than the wild-type promoter element for human HSF in vitro. Protein-DNA contact positions were identified by hydroxyl radical protection, diethyl pyrocarbonate interference, and DNase I footprinting. New contacts in the mutant HSE constructs corresponded to the locations of additional NGAAN motifs. The pattern of binding indicated the occurrence of multiple DNA binding modes for HSF with the various constructs and was consistent with an oligomeric, possibly trimeric, structure of the protein. In contrast to the improved binding, the extended heat shock element mutant constructs did not exhibit dramatically increased heat-inducible transcription in transient expression assays with HeLa cells.

Animals↗
PubMed1990

Determination of total body calcium by prompt gamma neutron activation analysis: absolute in vivo measurements.

Sequential TBCa measurements using the prompt gamma ray technique are firmly established and a number of patient groups are under investigation. A technique has been proposed for the absolute measurement of TBCa based upon the use of TBCl as an internal standard. Further work is needed to establish the validity of the method, but the initial results are encouraging.

Body Composition↗