Biomedical subjects
Publications and source records attributed to W Baier.
We have produced 25 clones secreting antibodies directed against chicken Calbindin D-28k. Two of them, 300 and 318, recognize determinants conserved in fish, chicken, mouse, rat, rabbit, monkey and human Calbindin D-28k. We demonstrate their use in the immunohistochemical localization of Calbindin D-28k, and in the detection of Calbindin D-28k on immunoblots.
The extensive studies pertinent to these problems cannot be elaborated here. We have restricted ourselves to a few representative concepts, which can be summarized as follows. The genetic aspects of convulsibility and epilepsy are highly complex phenomena. The level of convulsibility is determined by a number of different excitatory (and inhibitory) genetic factors. None of these factors is strictly specific to epilepsy. Each one is only a partial aspect of a complex genetic constitution which is strikingly common in perfectly healthy individuals, and which is related to a variety of psychic and somatic particularities. Almost all of these genetic factors seem to be polygenetically determined; in other words, they seem to reflect the actions of many genes. An increased liability to convulsions, and, finally, to epilepsy, is induced by an accumulation of these factorial sets, and, of course, by the effects of exogenous lesional factors. Special constellation of these polygenic sets may lead to the manifestation of different epileptic syndromes and may also explain the segregation of seizure types in the descendants of patients with seemingly uniform epileptic syndromes, as observed, for instance, in absence epilepsy and in juvenile myoclonic epilepsy.
We have produced twelve monoclonal antibodies (McAB) against carp-II parvalbumin. Three of them, designated 235, 239, 267 recognize determinants conserved in fish, chicken, mouse, rat, monkey and human parvalbumin. We show their use in the qualitative detection of parvalbumin (PV) by immunohistochemistry, in the quantitation of parvalbumin by radioimmunoassay and in the detection of parvalbumin on immunoblots.
In one 21-week-old fetus with prenatally diagnosed metachromatic leukodystrophy, galactolipid contents were determined in the forebrain cortex, cerebellum, brainstem, spinal cord, and kidney and compared to an appropriate control. Spinal cord and kidney showed the highest sulfatide accumulation as a consequence of deficient cerebroside sulfatase activity. No increase, but a measurable amount of sulfatide, was detected in the forebrain. The prenatal neural sulfatides contained a high proportion of the hydroxy fatty acid component. The galactosyl ceramides were not reduced in the early stage of the demyelinating disease.
Mouse hybridomas secreting antibodies to human insulin-like growth factor I (IGF I) were produced by fusion of spleen cells of hyperimmunised mice with FO mouse-myeloma cells. Eight clones producing antibodies against human IGF I have been isolated, two of which have been characterised. One was used in a radioimmunoassay, the other for immunopurification of IGF.
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The EEG of 38 patients suffering from primary generalized myoclonic astatic epilepsy since early childhood is studied in late stages of the disease. Spectral analysis shows that parietal 4-7 cps rhythms (theta rhythms) which are typical of the EEG in the early stages of the disorder can still exist in the EEG of the adult. The rhythms seem to be related to the course of the epilepsy. In the EEG of patients who still have seizures rhythms occur more often than in the EEG of patients who are free of seizures in the two years before the reexamination of their EEG. The functional anomaly producing a 4-7 cps rhythmization of the parietal EEG seems to be one pathogenetic factor in some epileptic disorders of early childhood, especially in primary generalized myoclonic-astatic epilepsy.
Cluster analysis is applied to power spectra of the EEG of 38 patients with a primary generalized myoclonic astatic epilepsy (Gundel et al. 1981). The tendency of the data to form clusters within this group is indicated by a random experiment which has been performed with the data. The clustering algorithm divided the material in seven distinct groups which may be combined to three main types of power spectra. These types are power spectra with a 10 cps peak, a 4-7 cps peak and power spectra without remarkable rhythmization. The comparison of EEG types and clinical data shows a correlation of 4-7 cps rhythms with the occurrence of seizures. 4-7 cps rhythms are interpreted as a symptom of "centrencephalic" convulsibility.
The growth-inhibiting behaviour of abacterial, liquid pure cultures made up of three water algae (Hydrodictyon reticulatum, Chlorella vulgaris, Aphanothece nidulans) which were made to grow profusely in special culture containers under constant exposure to light and ventilation was examined in ten different species of microorganisms during a period of contact of 4 days both in the light and in the dark. Subjected to the test were the 5 pathogenic species Staphylococcus aureus, Klebsiella aerogenes, Pseudomonas aeruginosa, Salmonella typhimurium and Candida albicans as well the 5 bacterial contamination indicators E. coli (faecal indicator), Streptococcus faecalis (enterococci), Enterobacter aerogenes ("coliforms"), Staphylococcus epidermidis (dermic germ) and Bacillus subtilis ("contamination germ"). It was found that --Hydrodictyon reticulatum and Aphanothece nidulans exert a strong antibacterial effect, while Chlorella vulgaris provides no indication of a bacterial growth-inhibiting effect. --this antibiosis is linked with the assimilative activity of the algal cultures, as in the dark no antibacterial action is discernible --the "antibiotic principle" must be liable to transitory or rapid disintegration because the algal cultures are ineffective in the dark and also culture filtrates of 5-days old algal cultures exercise no growth-inhibiting effect. With respect to the sanitation of waters it is important to state that a profuse growth of algae produces a certain, but hardly reliable "biological water disinfection" and renders difficult the assessment of the quality of a water-course as a result of the elimination of the usual contamination indicators. Therefore, additional microbiological quality parameters must be used for assessment of pollution of swimming pools with strong algal growth.
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