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W B Kristan

Publications and source records attributed to W B Kristan.

At least 19 recordsLinked to original sources
PubMed2000

Development of neuronal circuits and behaviors in the medicinal leech.

We are studying the neuronal mechanisms responsible for establishing circuitry underlying the local bending response in the medicinal leech. Local bending replaces an embryonic behavior, circumferential indentation, during the time of initial chemical synaptogenesis in leech embryos. We found that the electrical connections among the motor neurons are established first, about 5% of embryonic time (almost 2 full days) before chemical connections form. The inhibitory connections from muscle inhibitors to muscle excitors are, we hypothesize, responsible for the emergence of local bending. We have also found that the central processes of the excitors--but not the inhibitors--have much longer central processes when their peripheral processes are kept from contacting their target muscles. This system should allow us to test ideas about how individual neurons find their appropriate targets to form functional neuronal circuits.

Animals↗
PubMed2000

Disruption of peripheral target contact influences the development of identified central dendritic branches in a leech motor neuron in vivo.

Retrograde signaling from target tissues has been shown to influence many aspects of neuronal development in a number of developmental systems. In these experiments using embryonic leeches (Hirudo medicinalis), we examined how depriving a neuron of contact with its peripheral target affects the development of the cell's central arborization. We focused our attention on the motor neuron cell 3, which normally stimulates dorsal longitudinal muscle fibers to contract. At different locations in the periphery and in embryos of several different stages, we cut the nerve containing the growing axon of cell 3. This surgery led to dramatic overgrowth of cell 3's central dendritic branches, which normally accept synaptic contacts from other neurons, including the inhibitory motor neuron cell 1. When cell 3's peripheral axon was cut relatively early in development, its overgrown central branches eventually retracted. However, cells that were disrupted later in development retained their overextended branches into adulthood. In addition, if the axon was cut close to the ganglion early in development, depriving the cell of contact with any dorsal tissues, the central branches failed to retract and were instead retained into adulthood. Unlike cell 3, the central branches of cell 1, which has the same peripheral target muscles as cell 3, remained unchanged following all axotomy protocols. These results suggest that in at least some neurons contact with peripheral targets can influence development of the central processes that normally mediate synaptic contacts.

Aging↗
PubMed2000

Kinematics and modeling of leech crawling: evidence for an oscillatory behavior produced by propagating waves of excitation.

Many well characterized central pattern generators (CPGs) underlie behaviors (e.g., swimming, flight, heartbeat) that require regular rhythmicity and strict phase relationships. Here, we examine the organization of a CPG for leech crawling, a behavior whose success depends more on its flexibility than on its precise coordination. We examined the organization of this CPG by first characterizing the kinematics of crawling steps in normal and surgically manipulated animals, then by exploring its features in a simple neuronal model. The behavioral observations revealed the following. (1) Intersegmental coordination varied considerably with step duration, whereas the rates of elongation and contraction within individual segments were relatively constant. (2) Steps were generated in the absence of both head and tail brains, implying that midbody ganglia contain a CPG for step production. (3) Removal of sensory feedback did not affect step coordination or timing. (4) Imposed stretch greatly lengthened transitions between elongation and contraction, indicating that sensory pathways feed back onto the CPG. A simple model reproduced essential features of the observed kinematics. This model consisted of an oscillator that initiates propagating segmental waves of activity in excitatory neuronal chains, along with a parallel descending projection; together, these pathways could produce the observed intersegmental lags, coordination between phases, and step duration. We suggest that the proposed model is well suited to be modified on a step-by-step basis and that crawling may differ substantially from other described CPGs, such as that for swimming in segmented animals, where individual segments produce oscillations that are strongly phase-locked to one another.

Animals↗
PubMed1999

Dendritic Ca(2+)-activated K(+) conductances regulate electrical signal propagation in an invertebrate neuron.

Activity-dependent changes in the short-term electrical properties of neurites were investigated in the anterior pagoda (AP) cell of leech. Imaging studies revealed that backpropagating Na(+) spikes and synaptically evoked EPSPs caused Ca(2+) entry through low-voltage-activated Ca(2+) channels that are distributed throughout the neurites. Voltage-clamp recordings from the soma revealed a TEA-sensitive outward current that was reduced when Ca(2+) entry was blocked with Co(2+) or when the intracellular concentration of free Ca(2+) was reduced by a high-affinity Ca(2+) buffer. Ca(2+) released in the neurite from a caged Ca(2+) compound caused a hyperpolarization of the membrane potential. These data imply that the AP cell expresses Ca(2+)-activated K(+) conductances, and that these conductances are present in the neurites. When the Ca(2+)-activated K(+) current was reduced through the block of Ca(2+) entry, backpropagating Na(+) spikes and synaptically evoked EPSPs increased in amplitude. Hence, the activity-dependent changes in the intracellular [Ca(2+)] together with the Ca(2+)-activated K(+) conductances participate in the regulation of dendritic signal propagation.

Action Potentials↗
PubMed1999

Supralinear summation of synaptic inputs by an invertebrate neuron: dendritic gain is mediated by an "inward rectifier" K(+) current.

Dendritic processing of glutamatergic synaptic inputs was investigated in the anterior pagoda cell of leech. We observed that below spike threshold, the amplitude of individual EPSPs decreased with hyperpolarization and that simultaneous stimulation of pairs of synaptic inputs leads to the supralinear summation of EPSPs. Voltage-clamp measurements revealed a hyperpolarization-activated, Ba(2+)-sensitive, fast, noninactivating K(+) conductance that depends on the external [K(+)]. These features are those of an "inward rectifier," Kir. Microsurgery experiments, in combination with electrophysiological measurements, revealed an inhomogeneous spatial distribution of the Kir conductance. Furthermore, on surgical removal of the neurites that contain the Kir conductance, the amplitude of EPSPs from the remaining synaptic inputs increased with hyperpolarization. A model cell, with the Kir conductance as the sole voltage-dependent conductance, reproduced qualitatively the observed voltage dependence of individual EPSPs as well as the supralinear summation of EPSP pairs.

2-Amino-5-phosphonovalerate↗
PubMed1999

Glial responses during evoked behaviors in the leech.

Glial cells can respond with membrane potential changes during electrically stimulated neuronal activity (Kuffler, Proc R Soc Lond B 168:1-21, 1967; Orkand, Oxford University Press, 1995). Their role in contributing to, or controlling, neural circuits underlying behaviors, however, is completely unknown. We have used semi-intact preparations of the leech Hirudo medicinalis, where behaviors can be elicited and monitored (Kristan et al., J Neurobiol 27:380-389, 1995), to record membrane responses of identified glial cells during whole-body shortening and during fictive swimming. Giant glial cells are located in the neuropil of segmental ganglia, where neuronal axons and dendrites establish numerous synaptic contacts (Coggeshall and Fawcett, J Neurophysiol 27:229-289, 1964). We report here that these glial cells hyperpolarize when the whole-body-shortening response is evoked but not during fictive swimming. To our knowledge, this is the first report that associates a specific behavior with glial cell responses.

Animals↗
PubMed1999

Identification of neural circuits by imaging coherent electrical activity with FRET-based dyes.

We show that neurons that underlie rhythmic patterns of electrical output may be identified by optical imaging and frequency-domain analysis. Our contrast agent is a two-component dye system in which changes in membrane potential modulate the relative emission between a pair of fluorophores. We demonstrate our methods with the circuit responsible for fictive swimming in the isolated leech nerve cord. The output of a motor neuron provides a reference signal for the phase-sensitive detection of changes in fluorescence from individual neurons in a ganglion. We identify known and possibly novel neurons that participate in the swim rhythm and determine their phases within a cycle. A variant of this approach is used to identify the postsynaptic followers of intracellularly stimulated neurons.

Animals↗
PubMed1999

Relative roles of the S cell network and parallel interneuronal pathways in the whole-body shortening reflex of the medicinal leech.

The whole-body shortening reflex of the medicinal leech Hirudo medicinalis is a withdrawal response produced by anterior mechanical stimuli. The interneuronal pathways underlying this reflex consist of the S cell network (a chain of electrically coupled interneurons) and a set of other, parallel pathways. We used a variety of techniques to characterize these interneuronal pathways further, including intracellular stimulation of the S cell network, photoablation of the S cell axon, and selective lesions of particular connectives (the axon bundles that link adjacent ganglia in the leech nerve cord). These experiments demonstrated that the S cell network is neither sufficient nor necessary for the production of the shortening reflex. The axons of the parallel pathways were localized to the lateral connectives (whereas the S cell axon runs through the medial connective). We used physiological techniques to show that the axons of the parallel pathways have a larger diameter in the anterior connective and to demonstrate that the parallel pathways are activated selectively by anterior mechanosensory stimuli. We also presented correlative evidence that the parallel pathways, along with activating motor neurons during shortening, are responsible for inhibiting a higher-order "command-like" interneuron in the neuronal circuit for swimming, thus playing a role in the behavioral choice between swimming and shortening.

Animals↗
PubMed1998

Staging of middle and late embryonic development in the medicinal leech, Hirudo medicinalis.

We present a description of the last half of embryonic development in the European medicinal leech, Hirudo medicinalis, based entirely on externally visible morphological features, and establish reliably observable stages during that development. Embryogenesis, from the time fertilized eggs are deposited in an eggcase (called a cocoon) to the emergence of juveniles from the cocoon, takes approximately 4 weeks at room temperature. The stages described in this paper extend from the completion of segmentation to the appearance of the final bands of pigmentation. Developmental stages are expressed as percentages of total embryonic developmental time. This staging table was constructed for embryos kept at 20 degrees C. In addition, the development of animals kept at 17 degrees C or at 24 degrees C was compared with those held at 20 degrees C. Development proceeds more quickly at higher temperatures. Because development in embryos held at higher or lower temperatures was linearly related to the stages determined for embryos held at 20 degrees C, the rate of development at any intermediate temperature can be predicted from the staging table at 20 degrees C by simple multiplication.

Animals↗
PubMed1998

Development of spontaneous and evoked behaviors in the medicinal leech.

ABSTRACT The ontogeny of behavior in an organism must reflect developmental events in the nervous system, and it thus provides a noninvasive measure of neuronal development. This approach may be particularly fruitful in the medicinal leech because the neuronal basis of several behaviors has been characterized in adult leeches, providing a rich background against which behavioral development can be interpreted. We have investigated the order in which behaviors arise during the period of embryonic development and have determined the time at which each behavior is first expressed. Some behaviors, such as lateral ridge formation, germinal plate bending, spiral twisting, and sidewinding, were produced spontaneously by embryos. Others, such as shortening, circumferential indentation, local bending, and elongation, occurred only when they were elicited by weak mechanical stimulation. Such stimulation rarely evoked a behavioral response in young embryos (at 45% of the time required for complete embryonic development, 45% ED), but by 80% ED embryos responded to nearly 100% of the stimuli presented. In embryos older than 50% ED, the behavior most frequently evoked by stimulation of the anterior end, the posterior end, or the rear sucker was shortening. Stimulation of the midbody usually evoked behavior other than shortening, illustrating that the body was behaviorally compartmentalized, at least in part. Some behaviors observed during embryogenesis are never seen in adult leeches. For example, in response to stimulation of the midbody, young embryos produced a behavior that we have called "circumferential indentation," whereas older embryos produced local bending, a response previously described for adults. The switch from circumferential indentation to local bending may signal the formation of new synaptic connections.

Animals↗
PubMed1998

Quantitative analysis of a directed behavior in the medicinal leech: implications for organizing motor output.

The local bend is a directed behavior produced by the leech, Hirudo medicinalis, in response to a light touch. Contraction of longitudinal muscles near the touched location results in a bend directed away from the stimulus. We quantify the relationship between the location of touch around the body perimeter and the behavioral output by using video analysis, muscle tension measurements, and electromyography. On average, the direction of the behavioral output differed from the touch location by <8% of the total body perimeter. We discuss our results in the context of two contrasting behavioral strategies: a Continuous strategy, in which the local bend is directed exactly opposite to stimulus location, and a Categorical strategy, in which there are four distinct bend directions, each elicited by stimuli given in a single quadrant of the body perimeter. To distinguish between these strategies, we delivered two competing stimuli simultaneously. The resulting behavioral output is best described by an average of the effects of each stimulus given alone and thus provides support for the Continuous strategy. We also use a simple model, based on anatomical and physiological data, to predict the responses of the known motor neurons to different stimulus locations. The model shows that the activation of two of the motor neurons (D and V) is inconsistent with a Categorical strategy. However, these neurons are known to be active during the local bend behavior. This result, along with our experimental observations, suggests that the local bend network uses a Continuous strategy to encode stimulus location and produce directed behavioral output.

Animals↗
PubMed1998

A neuronal network for computing population vectors in the leech.

The correlation of neuronal activity with sensory input and behavioural output has revealed that information is often encoded in the activity of many neurons across a population, that is, a neural population code is used. The possible algorithms that downstream networks use to read out this population code have been studied by manipulating the activity of a few neurons in a population. We have used this approach to study population coding in a small network underlying the leech local bend, a body bend directed away from a touch stimulus. Because of the small size of this network we are able to monitor and manipulate the complete set of sensory inputs to the network. We show here that the population vector formed by the spike counts of the active mechanosensory neurons is well correlated with bend direction. A model based on the known connectivity of the identified neurons in the local bend network can account for our experimental results, and is suitable for reading out the neural population vector. Thus, for the first time to our knowledge, it is possible to link a proposed algorithm for neural population coding with synaptic and network mechanisms in an experimental system.

Action Potentials↗
PubMed1998

Behavioral hierarchy in the medicinal leech, Hirudo medicinalis: feeding as a dominant behavior.

The effect of feeding behavior on other behaviors (swimming, crawling and shortening) was investigated in the leech, Hirudo medicinalis. The stimulus locations and intensities required to produce mechanically elicited behaviors were first determined in the non-feeding leech. Stimuli were delivered while the leech was in various body positions to determine whether stimulus location affected behavioral response. Response thresholds were determined for the mechanically elicited behaviors. The same stimuli were then applied to feeding leeches to determine if response thresholds had changed. A solution with NaCl and arginine was used to elicit feeding. The same sets of stimuli were applied at intervals for an hour after feeding, to determine the duration of feeding-induced changes in behavior. Depending on the body position and stimulus location, stimuli produced different combinations of behaviors that included shortening, swimming and crawling. Anterior stimuli generally elicited shortening, whereas posterior stimuli generally elicited crawling and swimming, with swimming more likely to ventral stimulation than to dorsal stimulation. Having the front sucker attached changed these behavioral patterns. During feeding, the response thresholds changed dramatically, from 3-5 V to greater than 9 V. This increase in threshold began with the start of feeding, even before ingestion commenced. Suppression of the behaviors lasted up to 1 h after the end of feeding, with the effect on swimming being the most pronounced and longest lasting.

Animals↗
PubMed1998

Representation of touch location by a population of leech sensory neurons.

To form accurate representations of the world, sensory systems must accurately encode stimuli in the spike trains of populations of neurons. The nature of such neuronal population codes is beginning to be understood. We characterize the entire sensory system underlying a simple withdrawal reflex in the leech, a bend directed away from the site of a light touch. Our studies show that two different populations of mechanosensory neurons each encode touch information with an accuracy that can more than account for the behavioral output. However, we found that only one of the populations, the P cells, is important for the behavior. The sensory representation of touch location is based on the spike counts in all of the four P cells. Further, fewer than three action potentials in the P cell population, occurring during the first 100 ms of a touch stimulus, may be required to process touch location information to produce the appropriately directed bend.

Action Potentials↗
PubMed1998

Long-lasting depolarization of leech neurons mediated by receptors with a nicotinic binding site.

The serotonergic Retzius neurons of the leech midbody ganglia respond in a complex manner to pressure pulses of acetylcholine (ACh) applied onto their soma with a fast depolarization followed by a slower hyperpolarization and an additional delayed long-lasting depolarization. The delayed depolarization is the subject of the present study. The delayed depolarization could be elicited by long (> 1 s) ACh pressure pulses or by short pulses (10 ms) of carbachol, nicotine and DMPP, but not by muscarinic agonists. It was inhibited by bath application of nicotine (10-100 mumol l-1), strychnine (100 mumol l-1) and atropine (10-100 mumol l-1). Nicotinic antagonists that blocked the fast depolarization and the slow hyperpolarization (100 mumol l-1 mecamylamine and d-tubocurarine) did not affect the delayed depolarization induced by carbachol. Partial replacement of the extracellular Na+ by glucamine caused a decrease in the amplitude of the response and a shift of its reversal potential to more negative values. Carbachol pulses applied to Retzius neurons of the ganglia innervating the reproductive segments elicited delayed depolarizations of much smaller amplitude than the ones recorded in Retzius neurons from standard segments. The delayed depolarization could be elicited by the application of short agonist pulses onto different loci over the surface of the ganglion, at a distance from the soma. Isolated cultured Retzius neurons did not exhibit the delayed depolarization although they readily expressed the earlier phases of the complex cholinergic response. Carbachol pulses applied to the soma of other neurons in the leech ganglion produced a variety of specific responses. The results suggest that the delayed depolarization was produced by the activation of a cationic conductance mediated by receptors with a pharmacological profile similar to that of the alpha 9 nicotinic receptors and was not a byproduct of the early phases of the cholinergic response. The response seemed to be initiated in the extensive neuropilar processes of the Retzius cell, enabling a persistent excitatory signal.

Acetylcholine↗
PubMed1997

The neuronal basis of the behavioral choice between swimming and shortening in the leech: control is not selectively exercised at higher circuit levels.

Swimming and the whole-body shortening reflex are two incompatible behaviors performed by the medicinal leech Hirudo medicinalis. We set out to examine the neuronal basis of the choice between these behaviors, taking advantage of the fact that the neuronal circuit underlying swimming is relatively well understood. The leech swim circuit is organized hierarchically and contains three interneuronal levels, including two upper levels of "command-like" neurons. We tested the responses of the swim circuit neurons to stimuli that produced shortening, using reduced preparations in which neurophysiological recording could be performed while behaviors were elicited. We found that the majority of the swim circuit neurons, including most of the command-like cells and all of the cells at the highest hierarchical level of the circuit, were excited by stimuli that produced shortening as well as by stimuli that produced swimming. Only a subset of neurons, at levels below the top, were inhibited during shortening; these included one of the command-like cells and an oscillator cell (an interneuron that is part of the central pattern generator for swimming). These results imply that the control of the choice between swimming and shortening is not exercised selectively at the higher levels of the swim circuit.

Action Potentials↗
PubMed1997

Population coding and behavioral choice.

Many individual behavioral acts are produced by the combined activity of large populations of broadly tuned neurons, and the neuronal populations for different behaviors can overlap. Recent experiments monitoring and manipulating neuronal activity during behavioral decisions have begun to shed light on the mechanisms that enable overlapping populations of neurons to generate choices between categorically distinct behaviors.

Animals↗