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Biomedical subjects

V Vullo

Publications and source records attributed to V Vullo.

At least 109 records · Page 6Linked to original sources
PubMed1984

Anti-Ia reactivity in sera from subjects with Entamoeba histolytica infection.

Sera from 15 patients with Entamoeba histolytica infection were tested for anti T-cell antibodies by assessing cross-reacting specificities with the antigens defined by an anti-Ia hybridoma antibody. T cells prepared by sheep erythrocyte rosetting were preincubated with the test sera and then with the anti-Ia antibody. Binding of the specific monoclonal antibody was assessed by rosetting with ox erythrocytes conjugated with goat anti-mouse IgG. Eight sera from amoebic patients were found to block the binding of monoclonal mouse hybridoma anti Ia-antibody to T cells. Blocking of anti-Ia binding was not due to Fc IgG receptor binding by immune complexes nor was it HLA-DR restricted. T cells pre-treatment with the amoebic sera positive for anti-Ia activity showed reduced activity when tested in the autologous mixed lymphocyte reaction (AMLR). The results of our study seem to suggest the existence of specific anomalies of immunoregulation during E. histolytica infection which may play a role in inducing immune disregulation in vivo.

Adult↗
PubMed1984

[Radial counter-immunoelectrophoresis in the determination of specific anti-hydatidosis antibodies. Preliminary observations].

Radial counter-immunoelectrophoresis was evaluated with sera from surgically-confirmed hydatidosis cases, patients with other parasitic diseases and healthy controls. Radial counter-immunoelectrophoresis was a sensitive as classic counter-immunoelectrophoresis based on the same positivity criterion and was equally specific for the immunodiagnosis of human hydatidosis. The procedure appears to be simple and rapid (the immunoprecipitation step requires just 5 minutes) and merits consideration.

Antibodies↗
PubMed1984

New trends in the rapid serodiagnosis of leptospirosis.

TR/Patoc slide test, ELISA and TIA (Thin-Layer Immunoassay) were evaluated for their use in a rapid serodiagnosis of human leptospirosis. The results obtained indicated that the TR/Patoc slide test is very sensitive and able to detect within a few minutes early antibodies against whichever serovar of leptospires, like the antigens of the Galton test. The value of the ELISA test in detecting antibodies at a low level is confirmed. The TIA assay, employed here for the first time for the diagnosis of leptospirosis, though sensitive seemed too long to perform and consequently it is not a test of choice for a rapid screening of sera.

Agglutination Tests↗
PubMed1983

Detection and partial characterization of circulating immune complexes in hydatid disease.

Thirty sera from eight patients with disseminated or localized hydatid disease have been examined for the presence of circulating immune complexes (CICs) by the conglutinin-binding assay and for immunoglobulin levels. The highest levels of CICs were of the immunoglobulin A (IgA) class, with lower values of IgG-CIC and IgM-CIC; these results did not correlate, except for IgG, with the free immunoglobulin levels. Efforts to identify parasitic antigen(s) involved in the CIC formation with different methods have been unsuccessful. In the follow-up of each patient, CIC appeared to be better correlated to clinical conditions than to hemagglutination titers. We have concluded that the presence of CIC in hydatid disease is probably an expression of B-cell polyclonal activation and that these complexes are valuable in the clinical monitoring of the disease.

Antigen-Antibody Complex↗
PubMed1982

[Evaluation of ELIEDA test for immunodiagnosis of hydatid disease].

An ELIEDA test for hydatidosis was evaluated with sera from preoperative, surgically-confirmed hydatidosis cases, postoperative hydatidosis patients, persons with other parasitic disease and healthy donors. ELIEDA was more sensitive than the counter-immunoelectrophoresis test based on the same positivity criterion and was equally specific for the immunodiagnosis of human hydatidosis. The procedure appears to be simple and rapid and merits consideration in that it determines specific classes of antibodies involved in the immune response which appear to be useful in following the evolution of hydatid cysts.

Adult↗
PubMed1981

[The enzyme-linked immunosorbent assay (ELISA) test in the immunodiagnosis of urinary schistosomiasis. Comparison with the indirect haemagglutination (IH) and counterimmunoelectrophoresis (CIEP) (author's transl)].

Sera from patients with urinary schistosomiasis were used to compare the sensitivity and specificity of the ELISA, IH and CIEP tests for the detection of antibodies to schistosomes. Sera from persons with parasitic infections other than schistosomiasis, and sera from healthy persons were used as negative controls. The ELISA test was more sensitive than the IH and CIEP, and slightly more specific than the CIEP. This simple test may be suitable for use in large seroepidemiological surveys.

Adolescent↗
PubMed1980

Indirect immunoperoxydase test in the serological diagnosis of urinary schistosomiasis.

The outcome of the indirect immunofluorescence test (IIF) and the indirect immunoperoxydase test (IIP) was compared in 60 serum samples of patients affected by haematobium schistosomiasis and in controls. There was a striking agreement (positive correlation in 86.6%). The IIP is a simpler and cheaper test than the IIF.

Fluorescent Antibody Technique↗
PubMed1979

[Indirect immunoperoxidase technic in the biological diagnosis of human parasitic disease].

Two serological techniques, immunoperoxidase (IP) and immunofluorescence (IFI), were used for the detection of specific antibodies in 60 sera from patients with schistosomiasis, in 44 sera from patients with hydatidosis, and 28 sera from patients with intestinal amebiasis or liver amebic abscess. The IP test correlated well with IFI. The advantage of IP technique compared to IFI are that it is more rapid, less laborious and is performed without technical equipment.

Dysentery, Amebic↗
PubMed1978

[Comparative evaluation of three techniques of passive haemoagglutination for the determination of HBsAg (author's transl)].

The AA. studied the reaction of indirect haemoagglutination for the determination of HBsAg in 157 sera from patients with viral hepatitis, with 3 differents commercial tests based on the agglutination by HBsAg, respectively, of sheep, turkey and human erythrocytes sensitized with antibody to HBsAg. All three techniques showed a high sensibility comparable to that of the RIA. Regarding specificity the best results were obtained with the turkey and human erythrocytes, while the sheep erythrocytes because of the presence of some aspecific positivity, need a confirmatory test.

Hemagglutination Tests↗
PubMed1977

[Detection of hepatitis B antigens by use of the immunoenzymatic method (ELISA)].

The Authors investigated HBs antigen with an immunoenzymatic method (E.L.I.S.A.) in the sera of RIA and I.H. HBsAg positive hepatitis patients, in the sera of RIA and I.H. HBsAg-negative hepatitis patients and in those of normal subjects. E.L.I.S.A. test supplied results almost superposable to those obtained by RIA and therefore it seems worthy to be included among the most reliable techniques to detect hepatitis B surface antigen.

Enzyme-Linked Immunosorbent Assay↗
PubMed1977

[Indirect micro-haemagglutination method with duck erythrocytes for the diagnosis of hydatid disease (author's transl)].

The AA. have compared the results obtained with a indirect micro-haemagglutination method with sheep erythrocytes with those obtained with the indirect micro-haemagglutination method with duck erythrocytes for the diagnosis of hydatid disease. The results obtained with the two methods were not different and there was a good correlation between both methods. The direct micro-haemagglutination method with duck erythrocytes appears to be a valid alternative of the method with sheep erythrocytes.

Animals↗