Taxonomical relationships Klebsiella-Enterobacter investigated by means of phage-bacteria systems.
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Biomedical subjects
Publications and source records attributed to V Voicescu.
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The significant proportion of microorganisms belonging to the Klebsiella genus among the causative agents isolated and subdifferentiation of the genus for epidemiological survey of infantile Klebsiella infections are dealt with in the present paper. The 28 Klebsiella strains isolated from cases of enteritis confirms the enteral tropism of these enterobacteria, especially among the infantile population. A proportion of 13.3% strains were identified in pure cultures obtained from feces and were considered as certainly enteropathogenic. The biochemical behaviour of the strains studied pointed to the ubiquity of the aerogenes biotype (76.9%) followed by the pneumoniae biotype (12.4%) and indol-positive Klebsiella strains (10.7%). Of the total 308 Klebsiella strains typed serologically with 80 OK capsular sera, 284 (92.2%) were listed in 63 serotypes, 13 of which (K7, K35, K47, K9, 530, K16, K1, K11, K18, K20, K37, K80, K i.e. 47.5%) were frequently identified in acute infantile cases of enterocolitis syndrome. Most of these serotypes are also considered endemo-epidemic by authors. Worthy of note among the Klebsiella types isolated in pure cultures from cases of infantile enteritis, was the prevalence of the aerogenes biotype, with a higher incidence of K1, K2, K16, K26, K44 serotypes, and of the indol-positive strains, with serotypes K13, K16, K65, K80.
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The present study reports on the results of capsular serotyping of Klebsiella strains isolated between 1971 and 1972, carried out in view of the subdivision of the genus. Serologic typing was performed with K1--K80 sera on 156 Klebsiella strains, isolated from in-hospital cases of extraenteral klebsiella infections. The 140 (90%) strains typed were listed in 34 capsular serotypes. Another 16 strains (10%) could not be typed because of their insufficient capsular coating. The following 13 serotypes were predominant: K4 (13.57%), K2 (7.85%), K16 (6.42%), K18 (6.42%), K27 (5%), K20 (4.28%), K24 (4.28%), K55 (4.28%), K68 (4.28%), K48 (3.55%), K15 (2.85%), K17 (2.85%), representing 70.71%. In the feces samples from the healthy controls serotypes K7, K14, K30, K1, K2, K24, K27, K47 etc. may be considered omnipresent. It has been demonstrated that in periods of minimal epidemiological survey, these serotypes from healthy carriers may initiate nosocomial infections. The present findings emphasize the accessibility and value of serologic typing of klebsiellas according to clinical and epidemiologic indications. The serologic type is a marker that can be included on the programme of systematic typing of in-hospital strains.