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Biomedical subjects

V V Radchenko

Publications and source records attributed to V V Radchenko.

14 recordsLinked to original sources
PubMed2005

Functional expression and properties of Sec14p-like protein with molecular mass 45 kD from rat olfactory epithelium.

cDNA of Sec14p-like water-soluble protein with molecular mass 45 kD from rat olfactory epithelium was expressed in Escherichia coli Rosetta cells. The expression product was purified by a two-step chromatographic procedure on DEAE-Sepharose and Sephacryl S-200. The identity of structural and functional characteristics of the recombinant and native proteins was demonstrated by CD, mass spectrometry, and Western blotting. Using several lipids immobilized on nitrocellulose membranes, it was shown that phosphatidylinositol-3,4,5-triphosphate is the specific ligand for the studied protein.

Animals↗
PubMed2003

[Effect of allotransplantation of embryonic pluripotent progenitor cells on dynamics of systemic arterial blood pressure in spontaneously hypertensive rats].

The influence of embryonic pluripotent progenitor cells on the dynamics of the systemic arterial pressure was examined in rats with genetically determined arterial hypertension. It has been established that the single intravenous administration of the embryonic pluripotent and progenitor cells to spontaneously hypertensive rats in the amount of 5 x 10(7) ml lead to the lowering of the systemic arterial pressure for one month.

Animals↗
PubMed2002

Recombinant human peroxiredoxin VI: preparation and protective properties in vitro.

cDNA of human peroxiredoxin VI, one of the recently discovered novel antioxidant proteins, was expressed in Escherichia coli cells. The expression product was obtained in water-soluble form and purified by a two-step chromatographic procedure using DEAE-Sepharose and Sephacryl S-200. According to CD data, the polypeptide chain of the recombinant human peroxiredoxin VI contains approximately 40% alpha-helical region and 30% beta-structure, which is the same as for native rat peroxiredoxin VI. The protective properties of the recombinant protein determined as its ability to prevent the inactivation of glutamine synthetase from E. coli in a model oxidation system were comparable with the protective properties of native rat peroxiredoxin VI.

Animals↗
PubMed2002

[Effect of irbesartan, an antagonist of AT-1 receptors for angiotensin II, on L-arginine metabolism in arterial hypertension].

Effects of an antagonist of AT-1 receptors for angiotensin-II (Ang-II) irbezantane on the NO-synthase and arginase ways of the metabolism of L-arginine were studied in plasma and erythrocytes of the patients with arterial hypertension. The intensity of the non-oxidative arginase way of L-arginine metabolism in plasma and erythrocytes has been shown to be inhanced at hypertension versus the normotensive patients, while the activity of the alternative oxidative NO-synthase way was reduced. Inhibiting AT-1 receptors for Ang-II with high-affinity antagonist irbezantane normalized the ratio between two alternative ways of L-arginine metabolism through inhibiting the arginase way and reciprocal activating the NO-synthase way both in human plasma and erythrocytes.

Angiotensin II↗
PubMed2002

[Effect of irbesartan--angiotensin II type I receptor inhibitor on oxidative metabolism of lipids in essential hypertension].

We evaluated the alters in plasma vasoconstriction eicosanoids (LTC4, TXB2) and diene conjugates (DC) levels in patients with essential hypertension (EH) after chronic (30 days, 235 mg per day) of irbezartane (inhibitor of ANG II type 1 receptor with prolongation action "Aprovel" from "Sanofi") oral administration. Patients with EH have significantly higher plasma both LTC4, TXB2 and DC levels then healthy controls. This imbalance can be beneficially modulated by chronic irbezartane ("Aprovel") administration. It is concluded that ANG II type 1 receptors can be involved in regulation of free arachidomc acid oxidation.

Angiotensin Receptor Antagonists↗
PubMed1979

[Not Available].

Explore the source record for details and available documents.

Child↗
PubMedDate not supplied

[A proteomic approach to study of the function of the Sec14p-like p45 protein].

We undertook a search for proteins interacting with protein p45 by the method of two-hybrid screening in order to determine the function of the Sec14p-like protein p45. A screening of the yeast library of rat lung cDNA, six proteins specifically activating the reporter genes of a two-hybrid system and 21 unlikely protein partners of protein p45 were identified. The most likely candidate for the role of a p45 partner is the surfactant protein C (Sftpc). These results and previous studies led us to the hypothesis that protein p45 fulfills its protective function by participating in the biogenesis of cell membranes. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2005, vol. 31, no. 3; see also http://www.maik.ru.

Animals↗
PubMedDate not supplied

[The effect of the long-term treatment with calcium antagonists on myocardial function in patients with hypertension].

Prolonged effect (6-12 months) of verapamil and nifedipine on the systolic and diastolic function of the myocardium was studied in 48 patients with hypertensive disease (stage II). It was established that in patients with a high ejection fraction (55% and more) the two drugs produce a distinct reduction of the pumping heart function. The two drugs increase the rate of early diastolic filling. They also may reduce the arterial pressure via different hemodynamic mechanisms: vasodilatation or via reduction of cardiac output.

Adult↗
PubMedDate not supplied

[A comparative analysis of the antihypertensive activity of calcium antagonists and adrenoblockers in long-term treatment].

Essential hypertension stage II was treated by calcium antagonists, alpha- and beta-adrenoblockers in 362 patients. The drugs were compared for hypotensive efficiency at rest and exercise. A significant hypotensive effect was achieved in 81%, 58%, 44%, 43% and 37% of patients treated with labetalol, nifedipine, nadolol, propranolol, diltiazem, respectively. An increase in verapamil daily dose from 240 to 600 mg led to a rise in efficacy from 27 to 75% without an increase in the number of side effects. The antihypertensive effect of the drugs is shown to persist in long-term treatment as shown by exercise tests and static loads.

Adrenergic alpha-Antagonists↗
PubMedDate not supplied

[Binding of phosphatidylinositol-3,4,5-triphosphate to 45-kDa sec14-like protein from the rat olfactory epithelium in liposomes].

The binding of phosphatidylinositol-3,4,5-triphosphate to a protein with molecular mass of 45 kDa from rat olfactory epithelium (p45) was investigated using a model membrane system. Liposomes containing a mixture of phospholipids (phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol-3,4,5-triphosphate) were used in the study. The binding of the protein to liposomes caused by its interaction with phosphatidylinositol-3,4,5-triphosphate was confirmed by cosedimentation and immunoblotting with chemiluminescent detection using monoclonal antibodies to the native protein p45. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2006, vol. 32, no. 3; see also http://www.maik.ru.

Animals↗