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Biomedical subjects

V V Malaitsev

Publications and source records attributed to V V Malaitsev.

8 recordsLinked to original sources
PubMed2005

Comparative characterization of cytokine production by concanavalin A-activated splenocytes from BALB/c and C57BL/6 mice after cold exposure.

The level of cytokines produced by ConA activated splenocytes was studied in male BALB/c and C57Bl/6 mice after single and repeated cold exposure (-20 degrees C, 3 min). Single cold exposure significantly decreased IL-2, -3, -4, -5, -10, -12, IFN-gamma production in BALB/c mice and decreased IL-2 content and increased TNF-alpha level in C57Bl/6 mice. Repeated cold exposure normalized the content of IL-2, -4, -10, -12, and IFN-gamma in BALB/c mice, which reflects the development of adaptive immune reactions. In C57Bl/6 mice IL-2, -3, -5, -10, -12, and IFN-gamma production remained significantly decreased, which attested to dysadaptive processes.

Animals↗
PubMed1978

Bone marrow suppressor B cells in vitro.

Marrow cells from intact CBA and (CBA x C57B1)F1 mice added to a culture of syngeneic splenocytes at its initiation suppressed the generation of anti-SRBC antibody-forming cells. Removal of cells bearing surface immunoglobulins and/or MBLA led to a substantial reduction in the marrow suppressive activity. Marrow cells from mice pretreated with five doses of hydroxyurea did not suppress the immune response of syngeneic splenocytes to SRBC. While the total number of cells in the marrow of such animals declined 5-fold, the relative content of Ig-positive cells was somewhat increased. The proportion of blast cells dropped from 10.3 to 2.4%. No blast cells bearing surface immunoglobulins were observed. The proportion of cells incorporating [3H]-thymidine fell 40-fold. We conclude that suppression of the immune response to SRBC in vitro is mediated by immature precursors of the B lymphocyte series which are present in the bone marrow of intact mice.

Animals↗
PubMed1978

[Immunoglobulin-positive cells in mouse bone marrow].

The content of Ig-bearing lymphocytes and their precursors in the mouse bone marrow was investigated 6 and 36 hours after the hydroxyurea treatment. Some increase of the B-cell content takes place in the trated bone marrow. Dividing and non-dividing B-cell precursors, except the stem cells, were practically absent.

Animals↗
PubMedDate not supplied

[Dynamics of interleukin 2 production in patients with diffuse toxic goiter under the influence of treatment].

The paper is devoted to the results of a study of IL-2 production by T-helpers in the course of treatment of patients with diffuse toxic goiter (DTG); 30 patients and 30 healthy controls were investigated. The diagnosis of DTG was confirmed by modern methods including radioimmunoassays. A study of the blood level (relative and absolute) of T-lymphocytes in the patients before therapy, during 2 and 12 mos. of thimazol therapy and during remission of disease showed its significant decrease as compared to the controls. IL-2 production in the blood lymphocyte culture supernatant of the DTG patients was assessed by testing on 3H-thymidine labeled ConA-T-blasts. A rise of the stimulation index before therapy and during 2 mos. of therapy, its normalization at the subsequent stage of therapy and the reduction in the patients with remission of thyrotoxicosis were established. A double control of IL-2 production (resulting from therapy and the level of thyroid hormones T3 and T4 decreasing during therapy) was assumed. The results of the study suggested the selectivity of affection of T-lymphocyte subpopulations in DTG and confirmed the preserving of T-helper function.

Adult↗