Four patterns of changes in the proliferative activity of T-lymphocytes in the lymphocyte blastogenesis test with phytohemagglutinin (PHA) are distinguished in children with acute respiratory viral infections (ARVI). The differences between these patterns are due to aggravated clinical severity of the infection process and enhancement of the cytokine reaction of macrophage monocytes. Comparison of immunological characteristics of response to ARVI in 4 groups of children showed that high reactivity of T-lymphocytes during the acute phase of disease (first and third variants) correlated with a relatively weak production of immunoglobulins and antiviral antibodies, while the suppression of T-lymphocyte response to PHA (second and fourth variants) is associated with expressed humoral profile of immune response by the level of immunoglobulin and antiviral antibody production. These data permit a hypothesis about the predominant generation of T x 1-like clones in children with the first and third variants of immune response and of T x 2-like clones in children with the second and fourth variants.

Adenovirus Infections, Human↗

PubMedDate not supplied

[The protective role of postvaccinal immunity in mumps in children].

The immunological study of children with infectious parotitis (IP) without complications and with such complications as pancreatitis, meningitis or orchitis in the glandular form was carried out. In accordance with the previously proposed principle, 4 types of immune response (IR) were established on the basis of differences in initial resistance and the IR profile: cell-mediated immunity (types I and III) and humoral immunity (types II and IV). The patients included nonvaccinated children, as well as children vaccinated on epidemic indications, 3-6, 7-9, 10 and more years before infection. The comparative analysis of the number of IP cases with and without complications in the groups of children, divided according to their immunization history and the type of IR, revealed that postvaccinal immunity in children vaccinated on epidemic indications (less than a month ago) or 3-6 years before infection had protective potential, sufficient for the prevention of complicated forms of IP. Immunity obtained 7-9 years ago was effective for the protection from IP complications only in cell-mediated, but not humoral IR. Postvaccinal immunity obtained more than 10 years ago did not ensure the decrease in the occurrence of complicated forms of IP (in comparison with that in nonvaccinated patients) in children with any type of IR.

Adolescent↗

PubMedDate not supplied

[Four levels (types) of immune reponse in children with viral parotitis infections].

Four levels (types) of immune response, differing by expression of cytokines (TNF-alpha, IL-1 beta, IL-4, and gamma-IFN) and immunoglobulins IgG2, IgG3, IgG4, and IgE) and by expression and time course of specific cell-mediated and humoral immune response, were detected in children with different clinical forms of mumps. Types 1 and 3 immune response are predominantly cell-mediated, while types 2 and 4 predominantly humoral during the acute phase of the disease. The cytokine and antigen-specific profiles of each type of immune response correlate with the severity of clinical course of mumps.

Antibodies, Viral↗

PubMedDate not supplied

[Adaptation mechanisms in the formation of the Corynebacterium diphtheriae carrier state].

The complex clinico-laboratory examination of 92 children aged 3-14 years with the localized form of stomatopharyngeal diphtheria and toxigenic C. diphtheriae (TCD) carrier state was carried out. The children were hospitalized in the clinic of drop infections during the period of 1996-1998. The hydrophobic properties, adhesive, lysozyme, antilysozyme and DNAase activities of 92 TCD strains, as well as the levels of the specific and nonspecific immunity of the macroorganism, were studied. The study revealed that the character of the infectious process in TCD carrier state was determined by the degree of the colonization activity of the infective agent. Direct correlation between the time of the persistence of TCD in the body and their hydrophobic properties, adhesive and antilysozyme activities (with r = 0.89-0.93) was established. The duration of TCD carrier state was found to be inversely related to the electrophysiological state of epithelial cells of the mucosa, the level of humoral specific antibacterial immunity and the content of local antidiphtheria IgA.

Adaptation, Physiological↗

PubMedDate not supplied

[The temperature sensitivity of epidemic influenza A viruses as a marker of immunogenicity of reassortant vaccinal strains].

The influence of ts-phenotype of epidemic viruses and of cold-adapted (CA) reassortant vaccines' strains, appropriately prepared, produced on the human immunogenicity was under investigation. A widespread variability of epidemic viruses' thermal sensitivity sign was established. It was shown that the CA reassortant vaccine strains, obtained through crossbreeding of attenuation donors and of thermally resistant epidemic viruses, are described by a higher immunogenicity. Therefore, the immunogenicity of live influenza vaccines (LIV) can be defined by the ts-phenotype of epidemic parent viruses, which must be sampled for the reassortant vaccine strains not only through searching for samples of antigenically actual viruses but also through search for non-ts-phenotype viruses.

Administration, Intranasal↗

PubMedDate not supplied

[Analytical-equipment method for the estimation of the organism health condition and all it's systems after radiation].

Software for estimation of the health state of human body and its systems on the basis of blood parameters is described. The software uses ACS-ENOFIT analytical model based on image recognition theory and cluster analysis. This analytical method makes it possible to assess the state of patient's health and the state of each of the 10 body systems. Thus, prophylactic X-ray examination based on measurement of blood parameters makes it possible to reveal various disorders at different stages of development.

Blood Chemical Analysis↗

PubMedDate not supplied

[Use of ACS-ENOFIT system for mass-scale examinations].

Typical procedures for introduction of the ACS-ENOFIT system into use in medical institutions are considered. The ACS-ENOFIT system can be used for health status monitoring on the basis of analysis of standard peripheral blood count.

Blood Cell Count↗

PubMedDate not supplied

[Characteristics of lipid composition, lipid peroxidation intensity and ATPase activity of lymphocytes in children with acute respiratory viral infections].

Content of total lipids, intensity of lipid peroxidation and activity of ATPases were studied in lymphocytes of children with acute respiratory-viral infection (ARVI). Decrease in the content of total lipids correlated with lipid peroxidation in ARVI. At the same time, a decrease in the activity of ATPases occurred due to a decrease in the content of total lipids and alteration in the phospholipid composition of lymphocyte membranes. At the period of convalescence the patterns studied tended to normalization only in children with non-complicated forms of ARVI. Active and slowly-developed lipid peroxidation in lymphocytes of various children groups suggest dissimilar alterations in the structure and composition of the cellular membranes, which increases our knowledge on pathogenesis of ARVI.

Acute Disease↗

PubMedDate not supplied

[Use of catabolic repression in the selection of the glucoamylase-producer Aspergillus niger].

The effect of various carbon sources and cAMP on the glucoamylase synthesis in Aspergillus niger was studied to find carbon sources repressed the enzyme synthesis and conditions for the selection of catabolite stable mutants. Maltose at a concentration of 0.5% stimulated the glucoamylase synthesis, but at a concentration of 4% it repressed not only the enzyme synthesis but the growth of the parental strain on the agar medium. The more active mutant 66 was obtained as a result of treatment of Asp. niger st 6 with NG. This mutant is able to grow on the Czapek's medium containing maltose at concentrations 4 or 6%. The mutant 66 produced about 2.9 times more glucoamylase than its parent when maltose was added at 0.5% concentration to the medium. The glucoamylase synthesis in the parental strain was completely repressed under repressing conditions, while the level of the mutant strain activity was 35% from the level of enzyme activity on the medium without the repressor. The addition of cAMP (5.10(-5] resulted in a partial release of maltose (4%) repression of the glucoamylase synthesis in both strains. The results obtained indicate a possibility to select Asp niger mutants with the partially derepressed glucoamylase synthesis. Other regulation mechanisms in addition to catabolite repression may be involved in the regulation of the glucoamylase synthesis.

Aspergillus niger↗

PubMedDate not supplied

[Effect of a number of preparations on the function of the immune system in experimental influenza in mice].

(CBA X C57B1) X F1 mice were sensitized intraperitoneally with sheep erythrocytes and infected with influenza A viruses: nonpathogenic Leningrad-77 (H1N1) or pathogenic PR8 (HON1), before or five days after administration into the oesophagus of sodium succinate, levamisole, complexes I (panangin, sodium succinate, sodium glutamate) and 2 (lipoic acid, phosphothyamine, riboflavin, sodium pantothenate). The number of rosette-forming cells (RFC) in the spleen at 7 and 14 days postinfection, antibody titres, interferon level in the blood, the amount of virus in the lungs, spleen and lung morphology were studied. All the preparations used were found to increase the number of RFC in the spleen. Most effective were levamisole before infection, sodium succinate after infection, combination thereof, complex I after infection.

Adjuvants, Immunologic↗