[Models of experimental pneumoconiosis and dust-induced bronchitis via inhalational exposure to dust].
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Biomedical subjects
Publications and source records attributed to V V Iaglov.
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In 35 cats by means of methods for the blood bed injection and revealing the pancreatic islets after K. Kramlinger, R. Mayrand and N. Lifson (1979) with an additional pikrofucsin staining, the microcirculatory bed of the pancreatic lobe has been studied at light and electron microscopic level. It is presented as arterioles, precapillary arterioles, capillaries of the pancreatic islets and the exocrinic part of the gland, postcapillary venules and venules. The precapillary arterioles always branch into capillaries within the limits of the pancreatic islets. Position of the pancreatic islets, structure of the capillaries surrounding the acini, that of the postcapillaries and connected with them pericapillary and intercellular spaces make it possible for hormones of the endocrinic cells to produce local (paracrinic) effects on the cellular elements of the pancreas.
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Endocrine component of the crucian (Carassius carassius), carp (Cyprinus carpio), tench (Tinca tinca) and silurus (Silurus glanis) pancreas is structurally organized in the form of pancreatic islets. Gorbusha (Oncorhynchus gorbuscha) has, besides the islets, some Brockman's bodies. Endocrine component of the pancreas of Teleostei possesses A-, B-, D and acinar-islet cells "B". All types of cells are shoot-shaped and all have contacts with the capillaries. Extrusion of the hormones from the endocrine cells is carried out via emiocytosis, and in gorbusha at the time of migration--by microapocrine means. Secretory granules were observed to get into the capillaries and make hormonal storage necessary for fish migration. It was demonstrated that endocrine component of the pancreas in Teleostei is highly rich in innervation, neuronal fibers containing small granular vesicles.
After bilateral subphrenic vagotomy in male rats (in 7 and 45 days) there was revealed a sharp dilatation of the venous sinusoids, diminution of the chromaffin tissues area, and a more intensive chromaffin reaction in the adrenal medulla. Vagotomy resulted in a marked alteration of the ultrastructural organization of the secretory cells and the endothelium. Glucose administration to vagotomized animals caused only insignificant variations in adrenaline content in the chromaffin cells. Thus, vagotomy resulted in reduction of the functional activity of the adrenal medulla secretory cells.
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Roug's fixative fluid is suggested to fix the pancreas with subsequent staining of the islet A- and B-cells. Rouget's fluid differs from Bouin's fluid in that picric acid is substituted for distilled water, and thus, it is unnecesary to extract the acid from the preparation. The fixation ensures good quality in demonstration specific granulation and preserves it in B-cells. Gormori's modification of the method to stain the slices with aldehyd-fuchsin and subsequent staining with alum carmine and orange G reveals both B- and A-cells. The manipulations presented perfect the quality of histochemical reactions.
Methods of light and electron microscopy were used to study the structural organization of the endocrinous part of the pancreas in birds (domestic ducks and chickens). It has been established that the endocrinous part is formed by three types of pancreatic islands: dark, light and mixed islands. "Dark" islands consist of A- and D-cells, "light" ones--of B and D-cells and "mixed" islands--of A-, B- and D-cells. The index of B/A-cell ratio is equal to 0,39--0,4. This shows that each B-cell corresponds to 4 A-cells. On this basis one can say that the counterinsular apparatus of birds became stronger in the process of evolution and due to this a comparatively high level of glycemia is sustained in them. Availability of "dark" and "light" pancreatic islands also points out that in birds there occurred a partial disconnection of insular and counterinsular components of the endocrinous part of the pancreas, necessary for adaptation to principally new conditions of the environment.
The endocrinous part of the pancreas in the mammals under investigation (rats, guinea pigs, cattle, cats and man) comprises A-, B- and D-cells always disposed around capillaries were prevailing in all the animals. They excrete the secretory product immediately into the pericapillary gap. A-cells in rodents and man are located in the periphery of the island and often have no contacts with capillaries. The secretion from A- cells having no contacts with capillaries is released into intercapillary clefts into which the cytoplasmic spiculae of the adjacent exocrinous pancreacytes are turned, capable to overlap them and prevent from the entering of the hormone from the intercellular clefts into the pericapillary gap.
Intraperitoneal injection of alloxan to Testudo horsfieldi and Caspian tortoises in a dose of 300 mg/kg caused destructive and metabolic changes in the cell elements of both the endocrine and the exocrine part of the pancreas. Focal destruction occurred in the granular cytoplasmic reticulum of the acinar cells, and mitochondria--in the mucoid and centroacinar cells. Hydropic degeneration developed in B-cells. All the cell elements and nerve fibers displayed glycogen deposition, the most pronounced in the centroacinar and mucoid cells.
Electron-microscopic study of zona fasciculata and zona reticularis of the adrenal cortex showed that 7 days after bilateral subphrenic vagotomy there occurred a diatation of the perinuclear space of adrenocorticocytes and endothelial cells; there was also a dilatation of the tubules in the smooth cytoplasmic reticulum, edema of the mitochondria, reduction of their cristae, and increase in the number and size of lipid drops. In 45 days there are encountered mitochondria with the beginning myelinization, lipid drops form accumulations, and electron-transparent vacuoles are revealed in them. The functional activity of adrenocorticocytes in zona fasciculata and zona reticularis of the adrenal cortex diminished in rats after vagotomy.
Electron microscopic study of the pancreas of the vertebrates showed that asynchronous secretion of the pancreatic cells was regulated by means of the cytoplasmic extrusion. The latter provided selective entrance of the initial substances from the pericapillary spaces into the cells. Cytoplasmic extrusion also regulated the passage of the hormonal and other metabolites from the intercellular spaces into the pericapillary space.
It has been shown that the endocrinous part of the pancreas in amphibia is formed of A-, B- and D-cells. B-cells are always disposed around sinusoid capillaries, while A- and D-cells lie on the islet periphery and often have no contact with capillaries. In addition to islets in the pancreas there are remnants of the so-called "external" epithelium which is represented by solitary B-cells, located in the wall of small excretory ducts. In the periinsular zone there are numerous acino-islets of A-, B- and D-types among which B-cells are prevailing.
The endocrinous part of the pancreatic gland of reptiles Lacerta agilis, Agama sanguinolenta, Varanus griseus, Testudo horsfieldi and Clemmus Caspica is formed by A-, B- and D-cells. The main form of its structural organization is pancreatic islands. In adition to them, remnants of the "external epithelium" are found in Varanus griseus and A-cells disposed outside of the pancreatic islands in Testudo horsfieldi and Clemmys caspica. As compared with amphibia, reptiles have a better developed counterinsular component (A-cells) of the endocrinous part of the pancreas. The acino-island cells of "A" and "D" types are also found in the pancreas of reptiles, "A"-type cells being predominant among them.
The vascular part of the pancreas transport system is represented by capillary bed, pericapillary space and intercellular clefts. On the surface of the acinar cells and of the fibroblasts there are cytoplasmic plates facing the pericapillary space and the intercellular clefts. The vascular part of the pancreas transport system provides delivery of the products to the acinar cells, transport of the hormones, destructed cell structures and partly of digestive enzymes to the capillaries of the pancreas.
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