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Biomedical subjects

V V Gosteva

Publications and source records attributed to V V Gosteva.

23 records · Page 2Linked to original sources
PubMedDate not supplied

[The multiple effect of plasmid RP4::Mu cts 62 in transcipient bacilli].

Transfer of conjugative hybrid plasmid RP4::Mu cts 62 from Escherichia coli into Bac. cereus, Bac. thuringiensis, Bac. mesentericus and Bac. polymyxa cells led to the multiple effects on the structure and physiology of bacillus cells. It has resulted in a decrease of the bacillus vitality, in the accelerated autolytic decay of cells, in the delay of cell growth and reproduction rate in liquid and solid media, in the disruption of ultrastructure of the cell membrane and its surface layer.

Bacillus↗
PubMedDate not supplied

[Increase in the production of oncovirus type C by an L929 cell culture as a result of BCG infection].

The effect of BCG infection of L929 cells on replication of oncovirus type C was studied. Ultrathin sections of the BCG-infected culture were examined electron microscopically 1, 3, 6, 8, and 10 days postinfection. Most microorganisms with the morphology typical of mycobacteria were found inside phagosomes. The number of extracellular virions as well as budding and abnormal forms per one cell contour was counted. BCG-infected cells were found to produce significantly more virus than the controls. The difference was maximal 3 days postinoculation. Possible reasons for the increased oncovirus production by continuous cell lines after infection with BCG are discussed.

Animals↗
PubMedDate not supplied

[Morphological proof of vaccinia virus multiplication in enucleated cells].

Primary monkey kidney cells were enucleated by the combined effect of cytochalasin B and ultracentrifugation. The effectiveness of enucleation in the experiments was 96-99%. Cytoplasts after 1-hour restoration were inoculated with the WR strain of vaccinia virus at a multiplicity of 0.1, 1.0, 10, and 50 PFU/cell. Within 20-21 h postinoculation when a marked cytopathic effect of the virus was evident, the material was fixed for electron microscopy. Serial ultrathin sections of the infected material were examined. Careful examination of the infected cytoplasts revealed all the usual stages of the morphogenetic cycle of vaccinia virus, from virion absorption by the host cell to clumps of mature virus particles in the cytoplasm and extracellularly. Vaccinia virus is thought to have morphologically complete replication cycle in the cytoplasts.

Animals↗
PubMedDate not supplied

[The development of a Proteus mirabilis macrocolony on a solid nutrient medium].

As revealed in this study, the macrocolony of Proteus mirabilis, formed on solid culture medium, may consist both of the main part and of sporadically appearing dissociating subcolonies, considerably differing one from another. The outer edge of the main part of the macrocolony of swarming cells is represented by bacteria located in three perpendicular directions. The next intermediate area consisting of two layers is represented by bacteria oriented, as a rule, in one direction. The center of the colony is made up of short microbial cells. Between the upper layer and the surface of agar an original subpopulation of microbial cells, forming a separate layer, has been detected; together they determine the planar sandwich-like architectonics of the macrocolony.

Culture Media↗