Search PubMed⌕ Search

Biomedical subjects

V V Chernyĭ

Publications and source records attributed to V V Chernyĭ.

18 recordsLinked to original sources
PubMed2001

[Cholecystectomy by mini-laparotomy access. Retrospective analysis of the 10-year work].

Ten-year experience of operative treatment of cholelithic disease using mini-access cholecystectomy (MACH) in 920 patients was summarized. There were operated on 167 patients for an acute cholecystitis, chronic calculous cholecystitis--716, gall bladder polyposis--36, gall bladder cancer--1. In 48 patients the MACH was matched with choledochus drainage, in 9--choledocholithotomy was performed, in 1--choledochoduodenostomy. In 6 observations the MACH were done simultaneously inguinal herniotomy, in 10--umbilical herniotomy, in 7--uterine extirpation, in 13--operation for ovarial cyst. The bile leakage from gall bladder bed occurred in 3 observations, the operative wound suppuration--in 4. Duration of stationary treatment was 4.1 days at average.

Adolescent↗
PubMed2000

[Possibilities of transcutaneous intervention under ultrasonographic control in comprehensive treatment of severe necrotizing pancreatitis].

In 46 patients with an destructive acute pancreatitis (AP) the invasive intervention under control of ultrasonography, in 38 of them--in stage of purulent complications, was performed. It had promoted stabilization of state in 39 (89.8%) patients, normalization of clinic--laboratory indexes, that had permitted to realize radical operative intervention with minimum risk in perspective. Invasive intervention under control of ultrasonography--highly effective primary surgical manipulation in complex of treatment of the destructive AP severe forms.

Aged↗
PubMed1990

[Electrostatic method of determination of phospholipase A activity].

An electrostatic assay of phospholipase A activity has been developed. This assay is based on the monitoring of changes in the boundary potential of the planar lipid bilayer. The initial rate of the potential changes induced by phospholipase hydrolysis is proportional to the enzyme activity. The sensitivity of the method is up to 0.0002 Units/ml; the effect of impurities is ignorable. The method allows the use of natural phospholipase substrates and is rapid, i.e., the phospholipase activity assay takes approximately 5 minutes.

Electricity↗
PubMedDate not supplied

[Measurements of the potential difference during adsorption of phloretin and fluorescein on the surface of lipid membranes using the method of inner field compensation].

The inner field compensation method was used for the measurement of the boundary dipole potentials as a function of phloretin and fluorescein concentration, ionic strength and pH. These potentials were compared with calculated from the conductance change in the presence of nonactin. Both methods gave similar results for fluorescein, but the steady-state potentials in the case of phloretin, obtained by the first method were smaller. These results imply that fluorescein, being introduced on one side of the BLM, remained on that side contrary to phloretin which penetrated through the membrane and partitioned between its both boundaries.

Adsorption↗
PubMedDate not supplied

[Boundary potentials of lipid membranes in the presence of 1-anilino-8-naphthalene sulfonate ions].

Boundary and zeta potentials induced by ANS adsorption in dioleoyl lecithin membranes were measured using three methods, namely: inner membrane field compensation, measurements of carrier-mediated membrane conductance and microelectrophoresis. The changes of boundary potentials due to ANS adsorption recorded by the first two methods were the same and did not depend on the ionic strength of aqueous solutions. On the contrary, the values of zeta potentials were smaller as compared with the boundary potentials, and depended on the ionic strength. The results obtained were described satisfactorily by means of combination of Henry, Gouy-Chapman and Boltzmann equations, when ANS ions were assumed to adsorb at a plane shifted towards the hydrophobic region of the membrane.

Anilino Naphthalenesulfonates↗
PubMedDate not supplied

[Theory of hydrophobic ion adsorption in bilayer lipid membranes taking into account their lateral interaction and charge discreteness].

A relationship was calculated between the number of adsorbed hydrophobic ions and the potential created by them in BLM and concentrations of these ions in solution, ionic strength in solution, the value of the membrane charge and the potential dipole shift on the BLM surface. It was assumed in the model under consideration that the ion adsorption plane was located at some depth inside the membrane, the discrete nature of the ions, their possible lateral interaction and existence of various adsorption centres in the membrane being taken into account.

Ions↗
PubMedDate not supplied

[Adsorption of 1-anilino-3-naphthalenesulfonate on bilayer lipid membranes].

A study has been made of the dependence of a membrane potential produced by adsorbed ANS anions on their bulk concentration, on the ionic strength of the solution, on the magnitude of charge and dipole potential drop at the bilayer surface. Measurements have been made on artificial planar membranes of various composition by the inner field compensation method. On membranes of neutral lipids the ionic strength dependence was absent, while on charged BLM an increase in ionic strength resulted in a parallel shift of the potential dependence on ANS bulk concentration. A theory previously developed has been used for the interpretation of our results. It is assumed there that the adsorption plane of ANS ions is located within the membrane and account is taken of the discrete charge effect and the non-electric interaction of ions in the adsorption plane.

Adsorption↗